Histological and immunohistochemical characterization of the integument and parotoids glands Rhinella bergi (Anura: Bufsonidae): Development and differentiation

A detailed description of the tegument and parotoid glands of pre-metamorphic, post-metamorphic, juvenile and adult individuals of Rhinella bergi is presented to provide an exhaustive analysis of the integumentary characteristics of this species. Fragments of the tegument were fixed in Bouin solutio...

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Bibliographic Details
Published in:	Acta histochemica Vol. 121; no. 3; pp. 277 - 283
Main Authors:	Olea, Gabriela Beatriz, Cheij, Esteban Omar, Curi, Lucila Marilen, Cuzziol Boccioni, Ana Paula, Céspedez, Jorge Abel, Lombardo, Daniel Marcelo
Format:	Journal Article
Language:	English
Published:	Germany Elsevier GmbH 01-04-2019
Subjects:	Animals Anura - growth & development Anurans Cell Differentiation - physiology Dermis - ultrastructure Epidermal Cells - cytology Epidermis - ultrastructure Immunohistochemistry - methods Larva - growth & development Microscopy, Electron, Scanning - methods Ontogeny Skin - metabolism Skin - pathology Tegumentary annexes Anurans Tegumentary annexes Ontogeny
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Summary:	A detailed description of the tegument and parotoid glands of pre-metamorphic, post-metamorphic, juvenile and adult individuals of Rhinella bergi is presented to provide an exhaustive analysis of the integumentary characteristics of this species. Fragments of the tegument were fixed in Bouin solution and preserved in buffered Formol 10%. Subsequently, scanning electron microscopy (SEM) was performed to characterize the macroscopic structure of these regions. Microscopic observations were made from histological sections stained with Hematoxylin and Eosin, Alcian Blue (pH 2,5), PAS-H, Coomassie Blue, Oil Red, and Bielschowsky Impregnation.. There were three types of protuberance: warts, tubers, and thorns. These structures became evident from post-metamorphic stages. The ventral surface shows elevations similar to flat warts; however, tubers and spines are absent. Histologically, each structure consists of a spongy dermis of lax connective tissue and dense and compact dermis, associated with granular glands and a keratinized epidermis. The latter, in the dorsal region, forms projections called thorns. The granular glands accumulate, and their alveoli increase in size progressively. This work provides a morphological and histological description of the integument and the parotoid glands during the larval and post-metamorphic stage of the genus Rhinella, with aspects described for the first time in the genus.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0065-1281 1618-0372
DOI:	10.1016/j.acthis.2019.01.004