Search Results - "Chayen, Naomi"

Protein crystallization: from purified protein to diffraction-quality crystal by Saridakis, Emmanuel, Chayen, Naomi E

“…Determining the structure of biological macromolecules by X-ray crystallography involves a series of steps: selection of the target molecule; cloning,…”
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Crystallisation and characterisation of muscle proteins: a mini-review by Govada, Lata, Chayen, Naomi E.

“…The techniques of X-ray protein crystallography, NMR and high-resolution cryo-electron microscopy have all been used to determine the high-resolution structure…”
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Porous nucleating agents for protein crystallization by Khurshid, Sahir, Saridakis, Emmanuel, Govada, Lata, Chayen, Naomi E

“…A Protocol for obtaining high-quality protein crystals that involves addition to the protein solution of solid or semi-liquid nucleants that provide the…”
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Imprinted polymers assisting protein crystallization by Saridakis, Emmanuel, Chayen, Naomi E

“…Highlights • Molecularly imprinted polymers (MIPs) can be used to induce formation of protein crystals. • Proteins act as templates for their own…”
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Growing Crystals for X-ray Free-Electron Laser Structural Studies of Biomolecules and Their Complexes by Nanev, Christo N., Saridakis, Emmanuel, Chayen, Naomi E.

“…Currently, X-ray crystallography, which typically uses synchrotron sources, remains the dominant method for structural determination of proteins and other…”
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Protein crystal nucleation in pores by Nanev, Christo N., Saridakis, Emmanuel, Chayen, Naomi E.

“…The most powerful method for protein structure determination is X-ray crystallography which relies on the availability of high quality crystals. Obtaining…”
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Protein Crystals Nucleated and Grown by Means of Porous Materials Display Improved X-ray Diffraction Quality by Nanev, Christo N., Saridakis, Emmanuel, Govada, Lata, Chayen, Naomi E.

“…Well-diffracting protein crystals are indispensable for X-ray diffraction analysis, which is still the most powerful method for structure-function studies of…”
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Experimental localization of metal-binding sites reveals the role of metal ions in type II DNA topoisomerases by Wang, Beijia, Najmudin, Shabir, Pan, Xiao-Su, Mykhaylyk, Vitaliy, Orr, Christian, Wagner, Armin, Govada, Lata, Chayen, Naomi E, Fisher, L Mark, Sanderson, Mark R

“…Metal ions have important roles in supporting the catalytic activity of DNA-regulating enzymes such as topoisomerases (topos). Bacterial type II topos, gyrases…”
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Choosing the Method of Crystallization to Obtain Optimal Results by Govada, Lata, Chayen, Naomi

“…Anyone who has ever attempted to crystallise a protein or other biological macromolecule has encountered at least one, if not all of the following scenarios:…”
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Protein Crystallization and Biosensor Applications of Hydrogel-Based Molecularly Imprinted Polymers by Reddy, Subrayal M, Phan, Quan T, El-Sharif, Hazim, Govada, Lata, Stevenson, Derek, Chayen, Naomi E

“…We have characterized the imprinting capability of a family of acrylamide polymer-based molecularly imprinted polymers (MIPs) for bovine hemoglobin (BHb) and…”
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Turning protein crystallisation from an art into a science by Chayen, Naomi E

“…Protein crystallisation has gained a new strategic and commercial relevance in the post-genomic era because of its pivotal role in structural genomics…”
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Hydrophobic Interface-Assisted Protein Crystallization: Theory and Experiment by Nanev, Christo N, Saridakis, Emmanuel, Govada, Lata, Kassen, Sean C, Solomon, Hodaya V, Chayen, Naomi E

“…Macromolecular crystallization is crucial to a large number of scientific fields, including structural biology; drug design, formulation, and delivery;…”
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Protein crystallization facilitated by molecularly imprinted polymers by Saridakis, Emmanuel, Khurshid, Sahir, Govada, Lata, Phan, Quan, Hawkins, Daniel, Crichlow, Gregg V, Lolis, Elias, Reddy, Subrayal M, Chayen, Naomi E

“…We present a previously undescribed initiative and its application, namely the design of molecularly imprinted polymers (MIPs) for producing protein crystals…”
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Towards a ‘universal’ nucleant for protein crystallization by Saridakis, Emmanuel, Chayen, Naomi E

“…The expression ‘crystal clear’ stems from the science of crystallography, which determines the detailed atomic structures of materials by exposing crystals to…”
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Experiment and Theory for Heterogeneous Nucleation of Protein Crystals in a Porous Medium by Chayen, Naomi E., Saridakis, Emmanuel, Sear, Richard P.

“…The determination of high-resolution structures of proteins requires crystals of suitable quality. Because of the new impetus given to structural biology by…”
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Theoretical and experimental investigation of protein crystal nucleation in pores and crevices by Nanev, Christo, Govada, Lata, Chayen, Naomi E

“…The nucleation ability of pores is explained using the equilibration between the cohesive energy maintaining the integrity of a crystalline cluster and the…”
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Droplet Microfluidics XRD Identifies Effective Nucleating Agents for Calcium Carbonate by Levenstein, Mark A., Anduix‐Canto, Clara, Kim, Yi‐Yeoun, Holden, Mark A., González Niño, Carlos, Green, David C., Foster, Stephanie E., Kulak, Alexander N., Govada, Lata, Chayen, Naomi E., Day, Sarah J., Tang, Chiu C., Weinhausen, Britta, Burghammer, Manfred, Kapur, Nikil, Meldrum, Fiona C.

“…The ability to control crystallization reactions is required in a vast range of processes including the production of functional inorganic materials and…”
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Analysis of insulin glulisine at the molecular level by X-ray crystallography and biophysical techniques by Gillis, Richard B., Solomon, Hodaya V., Govada, Lata, Oldham, Neil J., Dinu, Vlad, Jiwani, Shahwar Imran, Gyasi-Antwi, Philemon, Coffey, Frank, Meal, Andy, Morgan, Paul S., Harding, Stephen E., Helliwell, John R., Chayen, Naomi E., Adams, Gary G.

“…This study concerns glulisine, a rapid-acting insulin analogue that plays a fundamental role in diabetes management. We have applied a combination of methods…”
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Inactivating mutations and X-ray crystal structure of the tumor suppressor OPCML reveal cancer-associated functions by Birtley, James R., Alomary, Mohammad, Zanini, Elisa, Antony, Jane, Maben, Zachary, Weaver, Grant C., Von Arx, Claudia, Mura, Manuela, Marinho, Aline T., Lu, Haonan, Morecroft, Eloise V. N., Karali, Evdoxia, Chayen, Naomi E., Tate, Edward W., Jurewicz, Mollie, Stern, Lawrence J., Recchi, Chiara, Gabra, Hani

“…OPCML , a tumor suppressor gene, is frequently silenced epigenetically in ovarian and other cancers. Here we report, by analysis of databases of tumor…”
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Random Microseeding: A Theoretical and Practical Exploration of Seed Stability and Seeding Techniques for Successful Protein Crystallization by Shaw Stewart, Patrick D, Kolek, Stefan A, Briggs, Richard A, Chayen, Naomi E, Baldock, Peter F. M

“…Microseed matrix-screening combined with random screens (rMMS) is a significant recent breakthrough in protein crystallization. In this study, a very…”
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