Identification of caffeic acid and rutin by UHPLC MS/MS and antioxidant activity of Commelina erecta Lineu. in cell culture

Identification of caffeic acid and rutin by UHPLC MS/MS and antioxidant activity of Commelina erecta Lineu. in cell culture

The Commelina erecta L. (C. erecta) also known as erva-de-santa-luzia is reported by local population to have medical properties against some pathological conditions. In this study, two extracts of C. erecta leaves (aqueous and ethanolic) were phytochemically analysed and evaluated for their in-vitr...

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Published in:Anais da Academia Brasileira de Ciências Vol. 92; no. 1; p. e20190491
Main Authors: Otsuka, Felipe A M, Santos, Rodrigo B, Chaves, Larissa F, Santos, Rosangela S, Chaves Filho, Adriano B, Miyamoto, Sayuri, Matos, Humberto R
Format: Journal Article
Language:English
Published: Brazil Academia Brasileira de Ciências 01-01-2020
Subjects:
Antioxidant activity
caffeic acid
Commelina erecta L
MULTIDISCIPLINARY SCIENCES
polyphenols
polyphenols
caffeic acid
Commelina erecta L
Antioxidant activity
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Summary:The Commelina erecta L. (C. erecta) also known as erva-de-santa-luzia is reported by local population to have medical properties against some pathological conditions. In this study, two extracts of C. erecta leaves (aqueous and ethanolic) were phytochemically analysed and evaluated for their in-vitro antioxidant activities by DPPH, TBARS, NO assays and cell viability assays. The ultra-high performance liquid chromatography followed by tandem mass spectrometry analysis showed the presence of rutin and caffeic acid in aqueous and ethanolic extract. The total polyphenols in aqueous and ethanolic extracts found were 142.7 ± 3.0 and 123.1 ± 5.8 μg/mL of GAE, respectively. The ethanolic extract (5 mg/mL) inhibits TBARS by 33.8%, and the aqueous extract (5 mg/mL) exhibited scavenger property against nitric oxide derivatives to an extent of 77.8%. In cell culture, both extracts improved cell survivability under H2O2 induced oxidative stress. Thus, C. erecta extract is a good candidate to become a phytotherapic medicine.
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ISSN:0001-3765
1678-2690
1678-2690
DOI:10.1590/0001-3765202020190491