Distinct classes and subclasses of antibodies to hemolysin co-regulated protein 1 and O-polysaccharide and correlation with clinical characteristics of melioidosis patients

Distinct classes and subclasses of antibodies to hemolysin co-regulated protein 1 and O-polysaccharide and correlation with clinical characteristics of melioidosis patients

Melioidosis is a tropical infectious disease caused by Burkholderia pseudomallei that results in high mortality. Hemolysin co-regulated protein 1 (Hcp1) and O-polysaccharide (OPS) are vaccine candidates and potential diagnostic antigens. The correlation of classes/subclasses of antibodies against th...

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Bibliographic Details
Published in:Scientific reports Vol. 9; no. 1; pp. 13972 - 14
Main Authors: Pumpuang, Apinya, Phunpang, Rungnapa, Ekchariyawat, Peeraya, Dulsuk, Adul, Loupha, Siriorn, Kwawong, Kochnipa, Charoensawat, Yaowaree, Thiansukhon, Ekkachai, Day, Nicholas P. J., Burtnick, Mary N., Brett, Paul J., West, T. Eoin, Chantratita, Narisara
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 27-09-2019
Nature Publishing Group
Subjects:
692/308/53/2421
692/699/255/1318
82/1
82/80
82/83
Antibodies, Bacterial - blood
Antigens
Burkholderia pseudomallei - immunology
Diabetes
Diabetes mellitus
Enzyme-linked immunosorbent assay
Female
Hemolysin Proteins - immunology
Humanities and Social Sciences
Humans
Immunoglobulin A
Immunoglobulin A - blood
Immunoglobulin G
Immunoglobulin G - blood
Immunoglobulin M
Immunoglobulins
Infectious diseases
Male
Melioidosis
Melioidosis - blood
Melioidosis - immunology
multidisciplinary
Polysaccharides
Polysaccharides - immunology
Science
Science (multidisciplinary)
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Summary:Melioidosis is a tropical infectious disease caused by Burkholderia pseudomallei that results in high mortality. Hemolysin co-regulated protein 1 (Hcp1) and O-polysaccharide (OPS) are vaccine candidates and potential diagnostic antigens. The correlation of classes/subclasses of antibodies against these antigens with clinical characteristics of melioidosis patients is unknown. Antibodies in plasma samples from melioidosis patients and healthy donors were quantified by ELISA and compared with clinical features. In melioidosis patients, Hcp1 induced high IgG levels. OPS induced high IgG and IgA levels. The area under receiver operating characteristic curve (AUROCC) to discriminate melioidosis cases from healthy donors was highest for anti-Hcp1 IgG (0.92) compared to anti-Hcp1 IgA or IgM. In contrast, AUROCC for anti-OPS for IgG (0.91) and IgA (0.92) were comparable. Anti-Hcp1 IgG1 and anti-OPS IgG2 had the greatest AUROCCs (0.87 and 0.95, respectively) compared to other IgG subclasses for each antigen. Survivors had significantly higher anti-Hcp1 IgG3 levels than non-survivors. Male melioidosis patients with diabetes had higher anti-OPS IgA levels than males without diabetes. Thus, diverse and specific antibody responses are associated with distinct clinical characteristics in melioidosis, confirming the diagnostic utility of these responses and providing new insights into immune mechanisms.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-48828-4