Search Results - "Chaput, John"

Redesigning the Genetic Polymers of Life by Chaput, John C

“…Conspectus Genomes can be viewed as constantly updated memory systems where information propagated in cells is refined over time by natural selection. This…”
Get full text

REVEALR: A Multicomponent XNAzyme-Based Nucleic Acid Detection System for SARS-CoV‑2 by Yang, Kefan, Chaput, John C

“…Isothermal amplification strategies capable of rapid, inexpensive, and accurate nucleic acid detection provide new options for large-scale pathogen detection,…”
Get full text

A biologically stable DNAzyme that efficiently silences gene expression in cells by Wang, Yajun, Nguyen, Kim, Spitale, Robert C., Chaput, John C.

“…Efforts to use RNA-cleaving DNA enzymes (DNAzymes) as gene-silencing agents in therapeutic applications have stalled due to their low efficacy in clinical…”
Get full text

What Is XNA? by Chaput, John C., Herdewijn, Piet

“…The term “xeno‐nucleic acids”, abbreviated XNA, has grown in popularity to the point that it has become a catch‐all phrase for almost any unnatural nucleic…”
Get full text

Orthogonal Genetic Systems by Chaput, John C., Herdewijn, Piet, Hollenstein, Marcel

“…Xenobiology is an emerging area of synthetic biology that aims to safeguard genetically engineered cells by storing synthetic biology information in…”
Get full text

Fluorescence-Activated Droplet Sorting for Single-Cell Directed Evolution by Vallejo, Derek, Nikoomanzar, Ali, Paegel, Brian M, Chaput, John C

“…Synthetic biology aims to improve human health and the environment by repurposing biological enzymes for use in practical applications. However, natural…”
Get full text

Darwinian evolution of an alternative genetic system provides support for TNA as an RNA progenitor by Yu, Hanyang, Zhang, Su, Chaput, John C.

“…The pre-RNA world hypothesis postulates that RNA was preceded in the evolution of life by a simpler genetic material, but it is not known if such systems can…”
Get full text

Improved synthesis and polymerase recognition of 7-deaza-7-modified α-l-threofuranosyl guanosine analogs by Barpuzary, Bhawna, Negria, Sergey, Chaput, John C

“…Threofuranosyl nucleic acid (TNA), an artificial genetic polymer known for its nuclease resistance and acid stability, has grown in popularity as a…”
Get full text

Transliteration of synthetic genetic enzymes by Wang, Yajun, Liu, Xiaolin, Shehabat, Mouhamad, Chim, Nicholas, Chaput, John C

“…Abstract Functional nucleic acids lose activity when their sequence is prepared in the backbone architecture of a different genetic polymer. The only known…”
Get full text

Crystallographic analysis of engineered polymerases synthesizing phosphonomethylthreosyl nucleic acid by Hajjar, Mohammad, Chim, Nicholas, Liu, Chao, Herdewijn, Piet, Chaput, John C

“…Xeno-nucleic acids (XNAs) are synthetic genetic polymers with backbone structures composed of non-ribose or non-deoxyribose sugars. Phosphonomethylthreosyl…”
Get full text

Evolution of a General RNA-Cleaving FANA Enzyme by Wang, Yajun, Ngor, Arlene K., Nikoomanzar, Ali, Chaput, John C.

“…The isolation of synthetic genetic polymers (XNAs) with catalytic activity demonstrates that catalysis is not limited to natural biopolymers, but it remains…”
Get full text

Generating Biologically Stable TNA Aptamers that Function with High Affinity and Thermal Stability by Dunn, Matthew R, McCloskey, Cailen M, Buckley, Patricia, Rhea, Katherine, Chaput, John C

“…Aptamers are often prone to nuclease digestion, which limits their utility in many biomedical applications. Here we describe a xeno-nucleic acid system based…”
Get full text

In Vitro Selection of an ATP-Binding TNA Aptamer by Zhang, Li, Chaput, John C

“…Recent advances in polymerase engineering have made it possible to isolate aptamers from libraries of synthetic genetic polymers (XNAs) with backbone…”
Get full text

Functionally Enhanced XNA Aptamers Discovered by Parallelized Library Screening by Lozoya-Colinas, Adriana, Yu, Yutong, Chaput, John C

“…In vitro evolution strategies have been used for >30 years to generate nucleic acid aptamers against therapeutic targets of interest, including…”
Get full text

Chemical evolution of an autonomous DNAzyme with allele-specific gene silencing activity by Nguyen, Kim, Malik, Turnee N., Chaput, John C.

“…Low activity has been the primary obstacle impeding the use of DNA enzymes (DNAzymes) as gene silencing agents in clinical applications. Here we describe the…”
Get full text

Parameterizing the Binding Properties of XNA Aptamers Isolated from a Low Stringency Selection by Kundu, Nandini, McCloskey, Cailen M, Hajjar, Mohammad, Chaput, John C

“…Machine learning offers a guided approach to aptamer discovery, but more information is needed to develop algorithms that can intelligently identify…”
Get full text

Crystal structures of a natural DNA polymerase that functions as an XNA reverse transcriptase by Jackson, Lynnette N, Chim, Nicholas, Shi, Changhua, Chaput, John C

“…Replicative DNA polymerases are highly efficient enzymes that maintain stringent geometric control over shape and orientation of the template and incoming…”
Get full text

Synthetic Genetic Polymers Capable of Heredity and Evolution by Pinheiro, Vitor B., Taylor, Alexander I., Cozens, Christopher, Abramov, Mikhail, Renders, Marleen, Zhang, Su, Chaput, John C., Wengel, Jesper, Peak-Chew, Sew-Yeu, McLaughlin, Stephen H., Herdewijn, Piet, Holliger, Philipp

“…Genetic information storage and processing rely on just two polymers, DNA and RNA, yet whether their role reflects evolutionary history or fundamental…”
Get full text

REVEALR-Based Genotyping of SARS-CoV‑2 Variants of Concern in Clinical Samples by Yang, Kefan, Schuder, Daniel N., Ngor, Arlene K., Chaput, John C.

“…The SARS-CoV-2 virus has evolved into new strains that increase viral transmissibility and reduce vaccine protection. The rapid circulation of these more…”
Get full text

Reply to: On gene silencing by the X10-23 DNAzyme by Spitale, Robert C., Chaput, John C.

Get full text