Search Results - "Chapman, M.D"

Monoclonal Antibodies for Defining Conformational Epitopes in Ara h 2 and Ara h 6 by Glesner, J, Wuenschmann, S, Koid, A, Mueller, G.A, Pedersen, L.C, Chapman, M.D, Pomes, A

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Purified Natural Ara h 6: An Important Marker for IgE Responses to Peanut by Koid, A.E, Chapman, M.D, Hamilton, R.G, Wünschmann, S

“…[...]it has been difficult to obtain natural Arah6 free of Arah2…”
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Variability Introduced into Allergen Immunoassays during the Dust Extraction Process by King, E.M, Block, D, Armstrong, J, Filep, S, Chapman, M.D

“…Highest levels of variability were observed for the 2mg dust aliquots, which ranged between 7% and 160%CV between triplicate aliquots…”
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An Improved Multiplex Array for Detection of Total and Allergen-specific IgE by King, E, Lekova, E, Smith, B, Chapman, M.D

“…Purified allergens or mAb were covalently coupled to fluorescent microspheres to develop a suspension array for detection of total IgE and IgE specific to Der…”
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Recombinant Der p 7 Constitutively Expressed in a Eukaryotic System Shows the Same Antigenic Properties as the Natural Allergen by Glesner, J, Chapman, M.D, Pomés, A

“…Two major bands of 22 and 26 kDa were observed in culture supernatants by silver-stained SDS-PAGE gels, corresponding to the 22.2 kDa predicted molecular…”
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Quantitation of Aspergillus versicolor Antigen Using ELISA and Fluorescent Multiplex Array Technology by Smith, B.R, Kespohl, S, Sander, I, Raulf-Heimsoth, M, King, E.M, Chapman, M.D

“…Aspergillus versicolor is an allergenic and potentially toxigenic mold found in damp indoor environments and water-damaged buildings…”
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IgE Antibody Binding Sites On Mite Group I Allergens Defined By X-ray Crystallography And Site-directed Mutagenesis by Pomés, A, Glesner, J, Vailes, L.D, Minor, W, Chruszcz, M, Chapman, M.D

“…Site-directed mutagenesis analysis of residues from epitopes identified by X-ray crystallography allows the location of amino acids important for…”
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Monoclonal Antibody and IgE Binding to Purified Natural Cry j 1 by Wünschmann, S, Brooks, B.P, Koid, A.E, Ichikawa, K, Chapman, M.D

“…Natural Cry j 1 was purified from defatted Japanese cedar pollen extract by Concanavalin A- and size exclusion chromatography…”
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American Cockroach Allergen rPer a 3 Expressed in Pichia pastoris by Glesner, J, Arruda, L.K, Chapman, M.D, Pomés, A

“…Per a 3, an American cockroach (Periplaneta americana) allergen shows homology to hemocyanin superfamily proteins and is reported to show skin test reactivity…”
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Structural Analysis of Der f 1 and Der p 1 Allergen-Antibody Complexes Explains Cross-reactivity of Group 1 Allergens by Chruszcz, M, Chapman, M.D, Vailes, L.D, Pomés, A, Minor, W

“…In both allergens the epitope is not only formed by the same amino acids, but their conformations are also the same. [...]the amino acids forming the epitope…”
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Optimization of a Multiplex Array for Indoor Allergens-Influence of Streptavidin-Phycoerythrin (SAPE) Lots on Assay Performance by Tandon, M, Chapman, M.D, King, E.M

“…The Multiplex Array for Indoor Allergens (MARIA) is a xMAP-based technology that allows the simultaneous detection of multiple allergens in a single test…”
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Purification of Native Shrimp Tropomyosin by Affinity Chromatography with Cross-reactive Antibody by Wünschmann, S, Uchida, M, Lehrer, S.B, Arruda, L.K, Chapman, M.D

“…Tropomyosin, the major allergen in shrimp shares a high degree of amino acid sequence identity and immunological cross-reactivity with other invertebrate…”
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Purification of Serine-protease Free nDer p 1 by Affinity Chromatography by Wuenschmann, S, Himly, M, Vailes, L.D, Maltais, R, Chapman, M.D

“…Proteolytic assays showed that serine protease activity, susceptible to inhibition with AEBSF (serine protease inhibitor), is present in mAb-affinity purified…”
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Peanut allergen (Ara h 1) detection in foods containing chocolate by Pomés, A, Vinton, R, Chapman, M D

“…Inadvertent exposure to peanut in foods poses health risks for peanut-allergic individuals that can be reduced by improving detection systems for allergen…”
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Identification of Cockroach Allergen using Proteomic Analysis by Arruda, L.K, Barbosa, M.C.R, Santos, A.B.R, Santos, L.D, Palma, M.S, Ferreira-Briza, F, Briza, P, Erler, A, Chapman, M.D, Pomés, A

“…Immunoblotting was performed using undiluted serum pool constructed with sera from 27 patients with asthma and/or rhinitis allergic to P. americana who…”
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The Der p 7 Crystal Structure Reveals Similarities to Innate Immune Proteins by Mueller, G.A, Edwards, L, Aloor, J.J, Fessler, M.B, Pomes, A, Chapman, M.D, London, R.E, Pedersen, L.C

“…Taken together with previous reports on Der p 2, the striking finding that two structurally distinct dust mite allergens are related to two different proteins…”
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Cross-reactivity Between Mite And Shrimp: The Effect Of Immunotherapy With Dust Mite Extract by Yang, A.C, Arruda, L.K, Santos, A.B.R, Barbosa, M.C.R, Chapman, M.D, Kalil, J, Castro, F.F.M

“…Cross-reactivity between food allergens and aeroallergens could lead to de novo sensitization following allergen-specific immunotherapy…”
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Quantitation of Stachybotrys chartarum Sch34 Antigen Using ELISA and Fluorescent Multiplex Array Technology by Smith, B.R, King, E.M, Belisle, D, Miller, J.D, Chapman, M.D

“…Stachybotrys chartarum is a mold associated with water-damaged buildings that can produce mycotoxins…”
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The X-Ray Crystal Structure of Two Complexes of the Cockroach Allergen Bla g 2 with Fragments of Monoclonal Antibodies Defines Two Non-overlapping Epitopes by Pomés, A, Li, M, Glesner, J, Wünschmann, S, King, E.M, Chapman, M.D, Wlodawer, A, Gustchina, A

“…Analysis of the crystal structure of Bla g 2-Fab/Fab'-mAb complexes enables the identification of antigenic determinants on the allergen and subsequent…”
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Sequence Analysis of Monoclonal Antibodies Against Group 1 Dust Mite Allergens by Williams, K.T, Chapman, M.D, Pomés, A

“…The highest variability in amino acid sequence is in the CDR2 and CDR3 from the heavy chain, and in length is in the CDR3 from heavy chain, which is thought to…”
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