Search Results - "Chapela, María José"

Development of a multiplex real-time PCR method for early diagnosis of three bacterial diseases in fish: A real-case study in trout aquaculture by Chapela, María-José, Ferreira, Martiña, Varela, Celia, Arregui, Luz, Garrido-Maestu, Alejandro

“…Lactococcus garvieae, Yersinia ruckeri and Flavobacterium psychrophilum are three of the most important pathogens in worldwide rainbow trout aquaculture…”
Get full text

In-house validation of a multiplex real-time PCR method for simultaneous detection of Salmonella spp., Escherichia coli O157 and Listeria monocytogenes by Garrido, Alejandro, Chapela, María-José, Román, Belén, Fajardo, Paula, Vieites, Juan M., Cabado, Ana G.

“…A wide variety of qPCR methods currently exist for Salmonella spp., Escherichia coli O157 and Listeria monocytogenes detection. These methods target several…”
Get full text

Application of real-time PCR to detect Listeria monocytogenes in a mussel processing industry: Impact on control by Garrido-Maestu, Alejandro, Chapela, María-José, Vieites, Juan M., Cabado, Ana G.

“…Real-Time PCR is a very powerful tool with multiple applications in food microbiology, being pathogen detection the most important for food safety. In early…”
Get full text

Presence of pathogenic Vibrio species in fresh mussels harvested in the southern Rias of Galicia (NW Spain) by Garrido-Maestu, Alejandro, Lozano-León, Antonio, Rodríguez-Souto, Rafael R., Vieites-Maneiro, Ramón, Chapela, María-José, Cabado, Ana G.

“…Spain is the third mussel producer worldwide, and the main supplier to the European market, coming 98% of this production from Galicia. Pathogenic species from…”
Get full text

lolB gene, a valid alternative for qPCR detection of Vibrio cholerae in food and environmental samples by Garrido-Maestu, Alejandro, Chapela, María-José, Vieites, Juan M., Cabado, Ana G.

“…In recent years a new genetic target for Vibrio cholerae detection has been reported, but its application was limited to clinical samples. This target, lolB,…”
Get full text

In-house validation of novel multiplex real-time PCR gene combination for the simultaneous detection of the main human pathogenic vibrios (Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus) by Garrido-Maestu, Alejandro, Chapela, María-José, Peñaranda, Elvira, Vieites, Juan M., Cabado, Ana G.

“…In recent years the incidence of vibriosis has greatly increased, raising the concern among consumers about the innocuity of certain food products. Previous…”
Get full text

Re-evaluation of Enhanced qPCR Prevalidated Method for Next-day Detection of Salmonella spp., Shigella spp., Escherichia coli O157 and Listeria monocytogenes by Garrido-Maestu, Alejandro, Chapela, María-José, Peñaranda, Elvira, Cabado, Ana G.

“…The current study was conducted in order to reduce the overall time needed in a previously validated qPCR method, and extend the procedure for the simultaneous…”
Get full text

Decrease of marine toxin content in bivalves by industrial processes by Reboreda, Antonio, Lago, Jorge, Chapela, María-José, Vieites, Juan M., Botana, Luis M., Alfonso, Amparo, Cabado, Ana G.

“…Harmful algal blooms cause important economical losses due to the accumulation of toxins in shellfish. Natural detoxification occurs but this mechanism is very…”
Get full text

Microalgae: A new source of healthy ingredients. Antioxidant and antimicrobial properties and incorporation as a functional ingredient for cheese making by Garrido, María José Chapela, Fajardo, Paula, Piñeiro, Enma Conde, Martínez, Mercedes Alonso, Farabegoli, Federica, Vilariño, José Manuel

“…Antimicrobial and antioxidant activities of nine species of microalgae have been determined to study its potential as healthy ingredients for functional foods…”
Get full text

Effectiveness of proteolytic enzymes to remove gluten residues and feasibility of incorporating them into cleaning products for industrial purposes by Fuciños, Clara, Estévez, Natalia, Míguez, Martín, Fajardo, Paula, Chapela, María José, Gondar, Dora, Rúa, M. Luisa

“…The development of protocols for efficient gluten elimination is one of the most critical aspects of any allergen management strategy in the industry. The…”
Get full text

Application of real-time PCR for early diagnosis of diseases caused by Aeromonas salmonicida, Vibrio anguillarum, and Tenacibaculum maritimum in turbot: A field study by Chapela, María-José, Ferreira, Martiña, Ruiz-Cruz, Asela, Martin-Varela, Iris, Fernández-Casal, Jacobo, Garrido-Maestu, Alejandro

“…In the present study a multiplex real-time PCR method was developed for early detection of diseased fish infected by Aeromonas salmonicida, Vibrio anguillarum,…”
Get full text

Detection of foodborne pathogens by qPCR: A practical approach for food industry applications by Chapela, María-José, Garrido-Maestu, Alejandro, Cabado, Ana G.

“…Microbiological analysis of food is an integrated part of microbial safety management in the food chain. Monitoring and controlling foodborne pathogens are…”
Get full text

Cyclic Imines: Chemistry and Mechanism of Action: A Review by Otero, Alberto, Chapela, María-José, Atanassova, Miroslava, Vieites, Juan M, Cabado, Ana G

“…In recent years, there has been an increase in the production of shellfish and in global demand for seafood as nutritious and healthy food. Unfortunately, a…”
Get full text

Microbiological Quality of Ready-to-Eat Pickled Fish Products by Atanassova, Miroslava R, Chapela, Maria-José, Garrido-Maestu, Alejandro, Fajardo, Paula, Ferreira, Martiña, Lago, Jorge, Aubourg, Santiago P, Vieites, J. M, Cabado, Ana G

“…The microbiological quality of 18 commercially available in Spain ready-to-eat fish products containing Engraulidae was evaluated through application of the…”
Get full text

Identification of Hake Species (Merluccius Genus) Using Sequencing and PCR−RFLP Analysis of Mitochondrial DNA Control Region Sequences by Quinteiro, Javier, Vidal, Rodrigo, Izquierdo, Mónica, Sotelo, Carmen G, Chapela, María José, Pérez-Martín, Ricardo I, Rehbein, Harmut, Hold, Georgina L, Russell, Valerie J, Pryde, Susan E, Rosa, Carla, Santos, Ana T, Rey-Méndez, Manuel

“…The use of DNA-based methodologies in identification of hake species belonging to the Merluccius genus was shown to be successful. A short fragment of the left…”
Get full text

A new multiplex real-time PCR developed method for Salmonella spp. and Listeria monocytogenes detection in food and environmental samples by Garrido, Alejandro, Chapela, María-José, Román, Belén, Fajardo, Paula, Lago, Jorge, Vieites, Juan M., Cabado, Ana G.

“…Despite efforts done by industries some well known foodborne pathogens, like Salmonella spp. and Listeria monocytogenes, continue to be a challenge to public…”
Get full text

Development of a multiplex real-time PCR method for pathogenic Vibrio parahaemolyticus detection (tdh+ and trh+) by Garrido, Alejandro, Chapela, María-José, Ferreira, Martiña, Atanassova, Miroslava, Fajardo, Paula, Lago, Jorge, Vieites, Juan M., Cabado, Ana G.

“…A multiplex real-time PCR (RT-PCR) method was developed in this work for pathogenic Vibrio parahaemolyticus detection in water and food samples. This bacterium…”
Get full text

Development of a multiplex real-time PCR method for simultaneous detection of Salmonella enterica, Shigella flexneri and Listeria monocytogenes in processed food samples by Garrido, Alejandro, Chapela, María-José, Román, Belén, Ferreira, Martiña, Lago, Jorge, Vieites, Juan M., Cabado, Ana G.

“…The present work is focused on the development of a TaqMan multiplex real-time PCR method for the detection of Salmonella , Shigella and L. monocytogenes in…”
Get full text

Comparison of DNA extraction methods from muscle of canned tuna for species identification by Chapela, María José, Sotelo, Carmen G., Pérez-Martín, Ricardo I., Pardo, Miguel Ángel, Pérez-Villareal, Begoña, Gilardi, Patricia, Riese, Juan

“…Four DNA extraction methods from canned tuna in different liquid media were compared. Wizard DNA Clean Up system with proteinase K previous digestion,…”
Get full text

Application of a novel pathogenicity marker in a multiplex real-time PCR method to assess total and pathogenic Vibrio vulnificus in food and environmental samples by Garrido-Maestu, Alejandro, Chapela, María-José, Román, Belén, Vieites, Juan M., Cabado, Ana G.

“…Pathogenic species of Vibrio genus, including Vibrio vulnificus, constitute a great challenge for food control agencies and a threat for consumers…”
Get full text