New insights into the genetic variability of Fasciola hepatica (Trematoda) in Algeria and relationships with other geographic regions revealed by mitochondrial DNA

New insights into the genetic variability of Fasciola hepatica (Trematoda) in Algeria and relationships with other geographic regions revealed by mitochondrial DNA

This study aims to investigate the level of genetic variability of flukes isolated from cattle in Algeria and to determine the phylogenetic and phylogeographic relationships with sequences isolated worldwide. Mitochondrial (Cytochrome c Oxidase subunit I gene - COI) and nuclear markers (Internal Tra...

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Bibliographic Details
Published in:Helminthologia Vol. 59; no. 2; pp. 152 - 164
Main Authors: Chaouadi, M., Scarpa, F., Azzena, I., Cossu, P., Harhoura, K., Aissi, M., Tazerouti, F., Garippa, G., Merella, P., Casu, M., Sanna, D.
Format: Journal Article
Language:English
Published: Heidelberg Sciendo 03-09-2022
Walter de Gruyter GmbH
Subjects:
Analysis
Cattle
COI
COI protein
Cytochrome-c oxidase
Cytochromes
Deoxyribonucleic acid
Divergence
DNA
Fasciola hepatica
Genetic analysis
Genetic diversity
Genetic structure
Genetic variability
Genetic variation
Haplotypes
ITS
Mitochondrial DNA
molecular characterization
Parasites
Phylogenetics
Phylogeny
Phylogeography
Population genetics
Ribosomal DNA
Algeria
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Summary:This study aims to investigate the level of genetic variability of flukes isolated from cattle in Algeria and to determine the phylogenetic and phylogeographic relationships with sequences isolated worldwide. Mitochondrial (Cytochrome c Oxidase subunit I gene - COI) and nuclear markers (Internal Transcribed Spacers of nuclear ribosomal DNA - ITS) for 24 flukes isolated from 12 cattle in North Algeria were characterised. Only two haplotypes were obtained for the COI gene, resulting in a low level of genetic variation. The analysis of variation among the COI sequences isolated from around the world did not show high levels of genetic divergence, and the phylogenetic analysis revealed a genetic similarity among isolates from different areas of the world. The analysis of the ITS region showed a low level of variability, which prevented obtaining informative phylogenetic and phylogeographic results. The present study also revealed that specimens of are genetically similar in different hosts, indicating that the genetic structure among populations of this parasite is not influenced by the host species. The low levels of genetic variation for COI and ITS regions among fluke isolates from all continents are consistent with a common origin for the flukes’ worldwide distribution.
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ISSN:1336-9083
0440-6605
1336-9083
DOI:10.2478/helm-2022-0021