Surface modification and initial adhesion events for intestinal epithelial cells

Surface modification and initial adhesion events for intestinal epithelial cells

Rapid resealing of the mucosal epithelia is imperative following injuries to the small intestine because the mucosa is responsible for the adsorption of nutrients as well as providing a barrier to noxious agents present in the lumen. Tissue engineering may provide a possible solution for treating in...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part A Vol. 76A; no. 2; pp. 272 - 278
Main Authors: Hagerman, E.M., Chao, S.H.H., Dunn, J.C.Y., Wu, B.M.
Format: Journal Article
Language:English
Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01-02-2006
Subjects:
Animals
cell adhesion
Cell Adhesion - drug effects
Cell Shape
cell signaling
Cells, Cultured
Fibronectins - pharmacology
Focal Adhesion Protein-Tyrosine Kinases - metabolism
Intestinal Mucosa - cytology
Intestine, Small - cytology
Phosphorylation
Polylysine - pharmacology
Rats
Signal Transduction
surface modification
Surface Properties
Tissue Engineering - methods
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Summary:Rapid resealing of the mucosal epithelia is imperative following injuries to the small intestine because the mucosa is responsible for the adsorption of nutrients as well as providing a barrier to noxious agents present in the lumen. Tissue engineering may provide a possible solution for treating intestinal erosions, ulcerations, inflammatory bowel disease, and infection. Cell‐biomaterial interaction is a critical component in tissue engineering that can determine the success of the tissue construct. Cell‐biomaterial interactions can be enhanced by various types of surface modification, which promote integrin ligation leading to increased cell function. In order to relate the effect of surface adhesion molecules to signaling events and macroscopic cell response, an intestinal epithelial cell line, IEC‐6, was plated on fibronectin (receptor‐mediated) and poly‐L‐lysine (non‐specific) surfaces. Focal adhesion kinase (FAK) phosphorylation, cell spreading, and cell adhesion strength were measured. Results showed increases in FAK phosphorylation generally corresponded to increases in cell spreading and adhesion strength for IEC‐6 cells. Therefore, in a simplified system, initial adhesion and signaling mechanisms appeared to correspond to subsequent physical responses in IEC‐6 cells relevant to tissue engineering applications. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006
Bibliography:ark:/67375/WNG-HP942BBJ-0
Fubon Foundation
ArticleID:JBM30562
istex:0989CF8EADC990DA1E7CDEEEE470A409F93CC977
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.30562