First Epidemiologic Study in Argentina of the Prevalence of BK Viruria in Kidney Transplant Patients

Abstract Background The worldwide seroprevalence of human BK polyomavirus (BKV) in adults is 80%. About 10%–60% of renal transplant recipients experience BKV infection, nephropathy of the graft may occur in 5% of the cases, and up to 45% lose the graft. The aim of this work was to describe the preva...

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Published in:	Transplantation proceedings Vol. 46; no. 9; pp. 3010 - 3014
Main Authors:	Schiavelli, R, Bonaventura, R, Rial, M.C, Petrone, H, Pujol, G. Soler, Gaite, L.J, Acosta, M, Gutierrez, A, Acosta, F, Valdez, G, Raffaele, P, Chanta, G, Perez, M, Potes, L, Suso, E, Cremades, G, Ibañez, J, Imperiali, N, Luxardo, R, Castellanos, M, Maggiora, E, Carreño, C. Agost, Cobos, M, Marinic, K, Sinchi, J.L, Otero, A.B, Freire, M.C
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-11-2014
Subjects:	Adult Argentina BK Virus - genetics BK Virus - isolation & purification Cross-Sectional Studies DNA, Viral - analysis Female Humans Immunosuppressive Agents - adverse effects Kidney Transplantation Male Middle Aged Opportunistic Infections - diagnosis Opportunistic Infections - epidemiology Opportunistic Infections - etiology Polyomavirus Infections - diagnosis Polyomavirus Infections - epidemiology Polyomavirus Infections - etiology Postoperative Complications - diagnosis Postoperative Complications - epidemiology Prevalence Real-Time Polymerase Chain Reaction Risk Factors Surgery Tumor Virus Infections - diagnosis Tumor Virus Infections - epidemiology Tumor Virus Infections - etiology Argentina
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Summary:	Abstract Background The worldwide seroprevalence of human BK polyomavirus (BKV) in adults is 80%. About 10%–60% of renal transplant recipients experience BKV infection, nephropathy of the graft may occur in 5% of the cases, and up to 45% lose the graft. The aim of this work was to describe the prevalence of BK viruria during the 1st year after transplantation. Methods An epidemiologic multicenter cross-sectional study was carried out in consecutive patients at each site with kidney transplantation from August 2011 to July 2012. Clinically significant viruria was defined as >107 copies/mL. Viral DNA was extracted with the use of silica columns. Quantification was performed with the use of real-time polymerase chain reaction with primers that amplify a fragment of the large T-antigen gene and with a specific Taqman-MGB probe for BKV. For each assay, a standard curve with a quantified plasmid was included. Results Of 402 renal transplant recipients at 18 renal transplant sites, we analyzed 382; median age was 46.33 years, and 46.40% were female. The median of the temporal distribution for urine samples was 153 days. BK virus was detected in 50/382 samples (13%), 18 with values >107 copies/mL (4.7%). The median of the distribution of positive values was 123 days and the highest frequency of positive values was in months 3–7. The conditions of recipient older than 34 years and donor older than 41 years were the only ones that showed statistically significant association with BK viruria. No association with any specific immunosuppressive drug was observed. Conclusions This is the first multicenter study conducted in Argentina to determine the prevalence of BK viruria in renal transplant recipients. Because of the growing number of the population susceptible to this infection, it is important to register and describe data about its epidemiology and associated risk factors.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0041-1345 1873-2623
DOI:	10.1016/j.transproceed.2014.07.009