Search Results - "Chandler, John E"

The effects of chemical fixation on the cellular nanostructure by Li, Yue, Almassalha, Luay M., Chandler, John E., Zhou, Xiang, Stypula-Cyrus, Yolanda E., Hujsak, Karl A., Roth, Eric W., Bleher, Reiner, Subramanian, Hariharan, Szleifer, Igal, Dravid, Vinayak P., Backman, Vadim

“…Chemical fixation is nearly indispensable in the biological sciences, especially in circumstances where cryo-fixation is not applicable. While universally…”
Get full text

Label-free imaging of the native, living cellular nanoarchitecture using partial-wave spectroscopic microscopy by Almassalha, Luay M., Bauer, Greta M., Chandler, John E., Gladstein, Scott, Cherkezyan, Lusik, Stypula-Cyrus, Yolanda, Weinberg, Samuel, Zhang, Di, Ruhoff, Peder Thusgaard, Roy, Hemant K., Subramanian, Hariharan, Chandel, Navdeep S., Szleifer, Igal, Backman, Vadim

“…The organization of chromatin is a regulator of molecular processes including transcription, replication, and DNA repair. The structures within chromatin that…”
Get full text

Multimodal interference-based imaging of nanoscale structure and macromolecular motion uncovers UV induced cellular paroxysm by Gladstein, Scott, Almassalha, Luay M., Cherkezyan, Lusik, Chandler, John E., Eshein, Adam, Eid, Aya, Zhang, Di, Wu, Wenli, Bauer, Greta M., Stephens, Andrew D., Morochnik, Simona, Subramanian, Hariharan, Marko, John F., Ameer, Guillermo A., Szleifer, Igal, Backman, Vadim

“…Understanding the relationship between intracellular motion and macromolecular structure remains a challenge in biology. Macromolecular structures are…”
Get full text

The Greater Genomic Landscape: The Heterogeneous Evolution of Cancer by Almassalha, Luay M, Bauer, Greta M, Chandler, John E, Gladstein, Scott, Szleifer, Igal, Roy, Hemant K, Backman, Vadim

“…Results have historically shown a broad plasticity in the origin of tumors and their functions, with significant heterogeneity observed in both morphologies…”
Get full text

High-speed spectral nanocytology for early cancer screening by Chandler, John E, Subramanian, Hariharan, Maneval, Charles D, White, Craig A, Levenson, Richard M, Backman, Vadim

“…High-throughput partial wave spectroscopy (HTPWS) is introduced as a high-speed spectral nanocytology technique that utilizes the field effect of…”
Get full text

Superresolution intrinsic fluorescence imaging of chromatin utilizing native, unmodified nucleic acids for contrast by Donga, Biqin, Almassalha, Luay M., Stypula-Cyrus, Yolanda, Urban, Ben E., Chandler, John E., Nguyen, The-Quyen, Sun, Cheng, Zhang, Hao F., Backman, Vadim

“…Visualizing the nanoscale intracellular structures formed by nucleic acids, such as chromatin, in nonperturbed, structurally and dynamically complex cellular…”
Get full text

Measuring Nanoscale Chromatin Heterogeneity with Partial Wave Spectroscopic Microscopy by Gladstein, Scott, Stawarz, Andrew, Almassalha, Luay M, Cherkezyan, Lusik, Chandler, John E, Zhou, Xiang, Subramanian, Hariharan, Backman, Vadim

“…Despite extensive research in the area, current understanding of the structural organization of higher-order chromatin topology (between 20 and 200 nm) is…”
Get more information

Sub-10-nm imaging of nucleic acids using spectroscopic intrinsic-contrast photon-localization optical nanoscopy (SICLON) by Eshein, Adam, Li, Yue, Dong, Biqin, Almassalha, Luay M, Chandler, John E, Nguyen, The-Quyen, Hujsak, Karl A, Dravid, Vinayak P, Sun, Cheng, Zhang, Hao F, Backman, Vadim

“…Elucidating chromatin structure in vitro requires resolution below 10 nm to visualize the mononucleosome has been an ongoing challenge. In this work, we…”
Get more information

Nanoscale refractive index fluctuations detected via sparse spectral microscopy by Chandler, John E, Cherkezyan, Lusik, Subramanian, Hariharan, Backman, Vadim

“…Partial Wave Spectroscopic (PWS) Microscopy has proven effective at detecting nanoscale hallmarks of carcinogenesis in histologically normal-appearing cells…”
Get full text

Stochastic fluorescence switching of nucleic acids under visible light illumination by Dong, Biqin, Almassalha, Luay M, Soetikno, Brian T, Chandler, John E, Nguyen, The-Quyen, Urban, Ben E, Sun, Cheng, Zhang, Hao F, Backman, Vadim

“…We report detailed characterizations of stochastic fluorescence switching of unmodified nucleic acids under visible light illumination. Although the…”
Get full text

Nanoscale chromatin imaging and analysis platform bridges 4D chromatin organization with molecular function by Li, Yue, Eshein, Adam, Virk, Ranya K A, Eid, Aya, Wu, Wenli, Frederick, Jane, VanDerway, David, Gladstein, Scott, Huang, Kai, Shim, Anne R, Anthony, Nicholas M, Bauer, Greta M, Zhou, Xiang, Agrawal, Vasundhara, Pujadas, Emily M, Jain, Surbhi, Esteve, George, Chandler, John E, Nguyen, The-Quyen, Bleher, Reiner, de Pablo, Juan J, Szleifer, Igal, Dravid, Vinayak P, Almassalha, Luay M, Backman, Vadim

“…Extending across multiple length scales, dynamic chromatin structure is linked to transcription through the regulation of genome organization. However, no…”
Get full text

Label free localization of nanoparticles in live cancer cells using spectroscopic microscopy by Spicer, Graham L C, Almassalha, Luay, Martinez, Ignacio A, Ellis, Ronald, Chandler, John E, Gladstein, Scott, Zhang, Di, Nguyen, The-Quyen, Feder, Seth, Subramanian, Hariharan, de la Rica, Roberto, Thompson, Sebastian A, Backman, Vadim

“…Gold nanoparticles (GNPs) have become essential tools used in nanobiotechnology due to their tunable plasmonic properties and low toxicity in biological…”
Get more information

Colocalization of cellular nanostructure using confocal fluorescence and partial wave spectroscopy by Chandler, John E., Stypula‐Cyrus, Yolanda, Almassalha, Luay, Bauer, Greta, Bowen, Leah, Subramanian, Hariharan, Szleifer, Igal, Backman, Vadim

“…A new multimodal confocal microscope has been developed, which includes a parallel Partial Wave Spectroscopic (PWS) microscopy path. This combination of…”
Get full text

Macrogenomic engineering via modulation of the scaling of chromatin packing density by Almassalha, Luay M., Bauer, Greta M., Wu, Wenli, Cherkezyan, Lusik, Zhang, Di, Kendra, Alexis, Gladstein, Scott, Chandler, John E., VanDerway, David, Seagle, Brandon-Luke L., Ugolkov, Andrey, Billadeau, Daniel D., O’Halloran, Thomas V., Mazar, Andrew P., Roy, Hemant K., Szleifer, Igal, Shahabi, Shohreh, Backman, Vadim

“…Many human diseases result from the dysregulation of the complex interactions between tens to thousands of genes. However, approaches for the transcriptional…”
Get full text

The transformation of the nuclear nanoarchitecture in human field carcinogenesis by Bauer, Greta M, Stypula-Cyrus, Yolanda, Subramanian, Hariharan, Cherkezyan, Lusik, Viswanathan, Parvathi, Zhang, Di, Iyengar, Radha, Bagalkar, Saurabh, Derbas, Justin, Graff, Taylor, Gladstein, Scott, Almassalha, Luay M, Chandler, John E, Roy, Hemant K, Backman, Vadim

“…Morphological alterations of the nuclear texture are a hallmark of carcinogenesis. At later stages of disease, these changes are well characterized and…”
Get full text

M1493: Steerable Ultrathin Cholangioscope Provides Wide-Field Trimodal Laser Imaging by Seibel, Eric J, Melville, Charles D, Lee, Cameron M, Chandler, John E, Naumann, Christopher R, Saunders, Michael D

Get full text

Calving sex ratio as related to the predicted Y-chromosome-bearing spermatozoa ratio in bull ejaculates by Chandler, John E., Taylor, Tara M., Canal, Anita L., Cooper, Richard K., Moser, E. Barry, McCormick, Michael E., Willard, Scott T., Rycroft, Herb E., Gilbert, Glen R.

“…The first objective was to correlate calving sex-ratio data from semen lots with the semen sex ratio obtained by two duplex polymerase chain reaction (PCR)/gel…”
Get full text

Evaluation of a novel, ultrathin, tip-bending endoscope in a synthetic force-sensing pancreas with comparison to medical guide wires by Chandler, John E, Lee, Cameron M, Babchanik, Alexander P, Melville, C David, Saunders, Michael D, Seibel, Eric J

“…Direct visualization of pancreatic ductal tissue is critical for early diagnosis of pancreatic diseases and for guiding therapeutic interventions. A novel,…”
Get full text

Collection frequency affects percent Y-chromosome bearing sperm, sperm head area and quality of bovine ejaculates by Chandler, John E, Canal, Anita M, Paul, J.B, Moser, E.B

“…This study evaluated the percentage of Y-chromosome bearing spermatozoal (%Y-CBS) variation from ejaculates within individual males using two experiments. In…”
Get full text

Abstract B11: Label-free hyperspectral microscopy detects alterations in nanoscale cellular structure with high temporal resolution by Chandler, John E., Almassalha, Luay M., Backman, Vadim

“…We have previously shown that analysis of the spectral variance of a reflected light microscope image allows direct study of cellular nanoscale organization…”
Get full text