Proteome profile and lentiviral transduction of cultured honey bee (Apis mellifera L.) cells

Honey bees (Apis mellifera L.) play a vital role in agriculture as pollinators, and serve as model organisms of social behaviour and immunity. The lack of both immortalized cell lines and methods to introduce recombinant DNA reliably into primary cells hinders cellular and molecular studies in this...

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Bibliographic Details
Published in:	Insect molecular biology Vol. 19; no. 5; pp. 653 - 658
Main Authors:	Chan, M.M.Y, Choi, S.Y.C, Chan, Q.W.T, Li, P, Guarna, M.M, Foster, L.J
Format:	Journal Article
Language:	English
Published:	Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-10-2010 Blackwell Publishing Ltd
Subjects:	Agriculture Animals Apis mellifera Bees - chemistry Bees - genetics cell culture Cells, Cultured Cryogenics DNA double prime L cells Embryos Gene Expression Genetic Vectors Green fluorescent protein Green Fluorescent Proteins - genetics honey bee honey bees Immunity Insect Proteins - analysis Insect Proteins - genetics L cells Lentivirus Lentivirus - genetics lentivirus transduction Oncogenes Pollinators Proteome - analysis proteomics Social behavior Transduction, Genetic - methods Transduction, Genetic - veterinary Transgenes
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Summary:	Honey bees (Apis mellifera L.) play a vital role in agriculture as pollinators, and serve as model organisms of social behaviour and immunity. The lack of both immortalized cell lines and methods to introduce recombinant DNA reliably into primary cells hinders cellular and molecular studies in this organism. We hereby demonstrate the expression of a GFP gene delivered by lentivirus transduction to cultured embryonic cells. The success of this approach indicates that viral transduction could be used to deliver constitutively active oncogenes in order to immortalize honey bee cells. We were able to revive cells successfully after several months of cryogenic storage and we show how the proteome varies between freshly collected and cultured embryonic cells.
Bibliography:	http://dx.doi.org/10.1111/j.1365-2583.2010.01022.x ArticleID:IMB1022 istex:DD2BCA5C6A4A3F596DB663AE7C8E56A9A99A9891 ark:/67375/WNG-FKRZJFT5-9 Both authors contributed equally to this work ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0962-1075 1365-2583
DOI:	10.1111/j.1365-2583.2010.01022.x