Search Results - "Champeil, P."

Amphipols From A to Z by Popot, J.-L, Althoff, T, Bagnard, D, Banères, J.-L, Bazzacco, P, Billon-Denis, E, Catoire, L.J, Champeil, P, Charvolin, D, Cocco, M.J, Crémel, G, Dahmane, T, de la Maza, L.M, Ebel, C, Gabel, F, Giusti, F, Gohon, Y, Goormaghtigh, E, Guittet, E, Kleinschmidt, J.H, Kühlbrandt, W, Le Bon, C, Martinez, K.L, Picard, M, Pucci, B, Sachs, J.N, Tribet, C, van Heijenoort, C, Wien, F, Zito, F, Zoonens, M

“…Amphipols (APols) are short amphipathic polymers that can substitute for detergents to keep integral membrane proteins (MPs) water soluble. In this review, we…”
Get full text

Involvement of the L6-7 loop in SERCA1a Ca2+-ATPase activation by Ca2+ (or Sr2+) and ATP by Lenoir, Guillaume, Picard, Martin, Møller, Jesper V, le Maire, Marc, Champeil, Philippe, Falson, Pierre

“…Wild-type (WT) and the double mutant D813A,D818A (ADA) of the L6-7 loop of SERCA1a were expressed in yeast, purified, and reconstituted into lipids. This…”
Get full text

Characterization of the Electrostatic Component of the Nucleotide Binding to Na,K-ATPase by FEDOSOVA, N. U., CHAMPEIL, P., ESMANN, M.

Get full text

The Cytoplasmic Loop Located between Transmembrane Segments 6 and 7 Controls Activation by Ca2+ of Sarcoplasmic Reticulum Ca2+-ATPase by Menguy, Thierry, Corre, Fabienne, Bouneau, Laurence, Deschamps, Stéphane, Møller, Jesper Vuust, Champeil, Philippe, le Maire, Marc, Falson, Pierre

“…During active cation transport, sarcoplasmic reticulum Ca2+-ATPase, like other P-type ATPases, undergoes major conformational changes, some of which are…”
Get full text

Projection Map of Covalently Phosphorylated Ca-ATPase from Tubular Crystals by DELAVOIE, F., McINTOSH, D., HENAO, F., PERANZI, G., CHAMPEIL, P., STOKES, D., LACAPÈRE, J-J.

Get full text

Characterization of a protease-resistant domain of the cytosolic portion of sarcoplasmic reticulum Ca2+-ATPase. Nucleotide- and metal-binding sites by Champeil, P, Menguy, T, Soulié, S, Juul, B, de Gracia, A G, Rusconi, F, Falson, P, Denoroy, L, Henao, F, le Maire, M, Moller, J V

“…Treatment of rabbit sarcoplasmic reticulum Ca2+-ATPase with a variety of proteases, including elastase, proteinase K, and endoproteinases Asp-N and Glu-C,…”
Get full text

Does a radiolabelled ligand bind to a homogeneous population of non-interacting receptor sites? by Swillens, S., Waelbroeck, M., Champeil, P.

“…Receptor subtypes of pharmacological interest are often characterized by studies in which a particular radiolabelled ligand is competitively displaced from its…”
Get full text

The Cytoplasmic Loop between Putative Transmembrane Segments 6 and 7 in Sarcoplasmic Reticulum Ca2+-ATPase Binds Ca2+ and Is Functionally Important by Falson, Pierre, Menguy, Thierry, Corre, Fabienne, Bouneau, Laurence, de Gracia, Adrienne Gomez, Soulié, Stéphanie, Centeno, Francisco, Møller, Jesper Vuust, Champeil, Philippe, le Maire, Marc

“…Limited proteolysis by proteinase K of rabbit SERCA1 Ca2+-ATPase generates a number of fragments which have been identified recently. Here, we have focused on…”
Get full text

Fast kinetics of calcium release induced by myo-inositol trisphosphate in permeabilized rat hepatocytes by Champeil, P, Combettes, L, Berthon, B, Doucet, E, Orlowski, S, Claret, M

“…We used a stopped-flow method for determining the kinetic properties (between 10 ms and 10 s) of the Ca2+ release induced by inositol 1,4,5-trisphosphate…”
Get full text

Ligand Binding to Macromolecules or Micelles: Use of Centrifugal Ultrafiltration to Measure Low-Affinity Binding by Menguy, Thierry, Chenevois, Stéphane, Guillain, Florent, le Maire, Marc, Falson, Pierre, Champeil, Philippe

“…We describe a method for estimating ligand binding to a macromolecular sample under conditions where this binding is of low affinity and must be measured under…”
Get full text

Interaction of membrane proteins and lipids with solubilizing detergents by le Maire, Marc, Champeil, Philippe, Møller, Jesper V

“…Detergents are indispensable in the isolation of integral membrane proteins from biological membranes to study their intrinsic structural and functional…”
Get full text

Calcium control on InsP3-induced discharge of calcium from permeabilised hepatocyte pools by Combettes, L, Claret, M, Champeil, P

“…The control exerted by intralumenal and cytosolic Ca2+ on InsP3-induced release of Ca2+ from intracellular Ca2+ pools in suspensions of saponin-permeabilised…”
Get more information

Mechanism of inhibition of the (Ca2+-Mg2+)-ATPase by nonylphenol by MICHELANGELI, F, ORLOWSKI, S, CHAMPEIL, P, EAST, J. M, LEE, A. G

Get full text

The metal sites on sarcoplasmic reticulum membranes that bind lanthanide ions with the highest affinity are not the ATPase Ca2+ transport sites by HENAO, F, ORLOWSKI, S, MERAH, Z, CHAMPEIL, P

“…We attempted to establish whether lanthanide ions, when added to sarcoplasmic reticulum (SR) membranes in the absence of nucleotide, compete with Ca2+ for…”
Get full text

Rapid kinetics of myo-inositol trisphosphate binding and dissociation in cerebellar microsomes by Hannaert-Merah, Z, Coquil, J F, Combettes, L, Claret, M, Mauger, J P, Champeil, P

“…Using sheep cerebellum microsomes adsorbed on a filter, we measured the kinetics of [3H]inositol 1,4,5-trisphosphate (InsP3) binding and dissociation on the…”
Get full text

Transient inositol 1,4,5-trisphosphate-induced Ca2+ release: a model based on regulatory Ca(2+)-binding sites along the permeation pathway by Swillens, S, Combettes, L, Champeil, P

“…A remarkable property of Ca2+ fluxes through the inositol 1,4,5-trisphosphate (InsP3)-gated Ca2+ channel is that successive increments of InsP3 induce repeated…”
Get full text

Kinetic characterization of the normal and detergent-perturbed reaction cycles of the sarcoplasmic reticulum calcium pump. Rate-limiting step(s) under different conditions by Champeil, P, le Maire, M, Andersen, J P, Guillain, F, Gingold, M, Lund, S, Møller, J V

“…We previously characterized the structural features of the interaction of sarcoplasmic reticulum membranes with nonsolubilizing concentrations of C12E8, the…”
Get full text

Characterization of the co-agonist effects of strontium and calcium on myo-inositol trisphosphate-dependent ion fluxes in cerebellar microsomes by Hannaert-Merah, Z., Combettes, L., Coquil, J.-F., Swillens, S., Mauger, J.-P., Claret, M., Champeil, P.

“…Using sheep cerebellum microsomes previously loaded with 45Ca 2+ or 90Sr 2+, we measured the dependence of inositol 1,4,5-trisphosphate (InsP 3)-induced efflux…”
Get full text

Phosphoenzymes formed from Mg.ATP and Ca.ATP during pre-steady state kinetics of sarcoplasmic reticulum ATPase by Orlowski, S, Lund, S, Møller, J, Champeil, P

“…We have investigated here the pre-steady state kinetics of sarcoplasmic reticulum ATPase incubated under conditions where significant amounts of Mg.ATP and…”
Get full text

Protective and Inhibitory Effects of Various Types of Amphipols on the Ca2+-ATPase from Sarcoplasmic Reticulum:  A Comparative Study by Picard, Martin, Dahmane, Tassadite, Garrigos, Manuel, Gauron, Carole, Giusti, Fabrice, le Maire, Marc, Popot, Jean-Luc, Champeil, Philippe

“…Amphipols are amphipathic polymers designed to replace or supplement detergents in membrane protein solution studies. Previous work has suggested both…”
Get full text