Accelerating the Morphogenetic Cycle of the Viral Vector Aedes aegypti Larvae for Faster Larvicidal Bioassays

Any bioassay to test new chemically synthesized larvicides or phytolarvicides against Culicidae and more harmful mosquito species, such as Aedes aegypti and Aedes albopictus, which specifically transmit dengue, yellow fever, chikungunya viral fevers as well as Zika virus, or Anopheles gambiae, a vec...

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Bibliographic Details
Published in:	BioMed research international Vol. 2020; no. 2020; pp. 1 - 9
Main Authors:	de Melo Rodovalho, Cynara, Golart, Jonas, da Silva, Mario Antonio Navarro, Chalcoski, Barbara Maria Santano, Wielewski, Leonardo Pellizzari, de Borba Dallagassa, Cibelle, Zuccolotto, Tatiana, Ferreira, Rafael Lopes, Fontana, José Domingos, Richardi, Vinicius Sobrinho
Format:	Journal Article
Language:	English
Published:	Cairo, Egypt Hindawi Publishing Corporation 2020 Hindawi Hindawi Limited
Subjects:	Adults Aedes - drug effects Aedes - growth & development Aedes - virology Aedes aegypti Animal Feed - analysis Animal Nutritional Physiological Phenomena Animals Bioassays Biological Assay - methods Biomedical research Casein Dengue fever Drug Evaluation, Preclinical - methods Eggs Female Food Food sources Glycerol Humans Insecticides - pharmacology Laboratories Larva - drug effects Larva - growth & development Larvae Larval development Larvicides Malaria Male Metamorphosis Metamorphosis, Biological Milk Molting Morphogenesis Mosquito Vectors - drug effects Mosquito Vectors - growth & development Mosquito Vectors - virology Mosquitoes Pupation Room temperature Vector-borne diseases Viruses Yeasts Zika virus Brazil
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Summary:	Any bioassay to test new chemically synthesized larvicides or phytolarvicides against Culicidae and more harmful mosquito species, such as Aedes aegypti and Aedes albopictus, which specifically transmit dengue, yellow fever, chikungunya viral fevers as well as Zika virus, or Anopheles gambiae, a vector for malaria and philariasis, requires thousands of well-developed larvae, preferably at the fourth instar stage. The natural morphogenetic cycle of Aedes spp., in the field or in the laboratory, may extend to 19 days at room temperature (e.g., 25°C) from the first permanent contact between viable eggs and water and the last stage of larval growth or metamorphosis into flying adults. Thus, accelerated sequential molting is desirable for swifter bioassays of larvicides. We achieved this goal in Aedes aegypti with very limited strategic and low-cost additions to food, such as coconut water, milk or its casein, yeast extract, and to a lesser extent, glycerol. The naturally rich coconut water was excellent for quickly attaining the population of instar IV larvae, the most advanced one before pupation, saving about a week, for subsequent larvicidal bioassays. Diluted milk, as another food source, allowed an even faster final ecdysis and adults are useful for mosquito taxonomical purpose.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Academic Editor: Ceferino M. López Sández
ISSN:	2314-6133 2314-6141 2314-6141
DOI:	10.1155/2020/7405421