Search Results - "Chait, Brian"

Mass Spectrometry: Bottom-up or Top-down? by Chait, Brian T.

“…A novel approach to mass spectrometry involving fragmentation of intact proteins in the gas phase promises to greatly improve our ability to determine protein…”
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Activation of DSB Processing Requires Phosphorylation of CtIP by ATR by Peterson, Shaun E., Li, Yinyin, Wu-Baer, Foon, Chait, Brian T., Baer, Richard, Yan, Hong, Gottesman, Max E., Gautier, Jean

“…DNA double-strand breaks (DSBs) activate a DNA damage response (DDR) that coordinates checkpoint pathways with DNA repair. ATM and ATR kinases are activated…”
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Structural Basis for Helicase-Polymerase Coupling in the SARS-CoV-2 Replication-Transcription Complex by Chen, James, Malone, Brandon, Llewellyn, Eliza, Grasso, Michael, Shelton, Patrick M.M., Olinares, Paul Dominic B., Maruthi, Kashyap, Eng, Edward T., Vatandaslar, Hasan, Chait, Brian T., Kapoor, Tarun M., Darst, Seth A., Campbell, Elizabeth A.

“…SARS-CoV-2 is the causative agent of the 2019–2020 pandemic. The SARS-CoV-2 genome is replicated and transcribed by the RNA-dependent RNA polymerase holoenzyme…”
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Nuclear ARP2/3 drives DNA break clustering for homology-directed repair by Schrank, Benjamin R., Aparicio, Tomas, Li, Yinyin, Chang, Wakam, Chait, Brian T., Gundersen, Gregg G., Gottesman, Max E., Gautier, Jean

“…DNA double-strand breaks repaired by non-homologous end joining display limited DNA end-processing and chromosomal mobility. By contrast, double-strand breaks…”
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Affinity Proteomics Reveals Human Host Factors Implicated in Discrete Stages of LINE-1 Retrotransposition by Taylor, Martin S., LaCava, John, Mita, Paolo, Molloy, Kelly R., Huang, Cheng Ran Lisa, Li, Donghui, Adney, Emily M., Jiang, Hua, Burns, Kathleen H., Chait, Brian T., Rout, Michael P., Boeke, Jef D., Dai, Lixin

“…LINE-1s are active human DNA parasites that are agents of genome dynamics in evolution and disease. These streamlined elements require host factors to complete…”
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Asymmetric Molecular Architecture of the Human γ-Tubulin Ring Complex by Wieczorek, Michal, Urnavicius, Linas, Ti, Shih-Chieh, Molloy, Kelly R., Chait, Brian T., Kapoor, Tarun M.

“…The γ-tubulin ring complex (γ-TuRC) is an essential regulator of centrosomal and acentrosomal microtubule formation, yet its structure is not known. Here, we…”
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Interactome Mapping Reveals the Evolutionary History of the Nuclear Pore Complex by Obado, Samson O, Brillantes, Marc, Uryu, Kunihiro, Zhang, Wenzhu, Ketaren, Natalia E, Chait, Brian T, Field, Mark C, Rout, Michael P

“…The nuclear pore complex (NPC) is responsible for nucleocytoplasmic transport and constitutes a hub for control of gene expression. The components of NPCs from…”
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Integrative structure and functional anatomy of a nuclear pore complex by Kim, Seung Joong, Fernandez-Martinez, Javier, Nudelman, Ilona, Shi, Yi, Zhang, Wenzhu, Raveh, Barak, Herricks, Thurston, Slaughter, Brian D., Hogan, Joanna A., Upla, Paula, Chemmama, Ilan E., Pellarin, Riccardo, Echeverria, Ignacia, Shivaraju, Manjunatha, Chaudhury, Azraa S., Wang, Junjie, Williams, Rosemary, Unruh, Jay R., Greenberg, Charles H., Jacobs, Erica Y., Yu, Zhiheng, de la Cruz, M. Jason, Mironska, Roxana, Stokes, David L., Aitchison, John D., Jarrold, Martin F., Gerton, Jennifer L., Ludtke, Steven J., Akey, Christopher W., Chait, Brian T., Sali, Andrej, Rout, Michael P.

“…Nuclear pore complexes play central roles as gatekeepers of RNA and protein transport between the cytoplasm and nucleoplasm. However, their large size and…”
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Modular assembly of the nucleolar pre-60S ribosomal subunit by Sanghai, Zahra Assur, Miller, Linamarie, Molloy, Kelly R., Barandun, Jonas, Hunziker, Mirjam, Chaker-Margot, Malik, Wang, Junjie, Chait, Brian T., Klinge, Sebastian

“…Cryo-electron microscopy structures of multiple states along the assembly pathway of the nucleolar pre-60S ribosomal subunit suggest that folding of the…”
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The complete structure of the small-subunit processome by Barandun, Jonas, Chaker-Margot, Malik, Hunziker, Mirjam, Molloy, Kelly R, Chait, Brian T, Klinge, Sebastian

“…The 3.8-Å cryo-EM structure of the Saccharomyces cerevisiae small-subunit processome in a state that precedes pre-rRNA cleavage at site A1 provides an…”
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Phosphorylation-dependent 14-3-3 binding to LRRK2 is impaired by common mutations of familial Parkinson's disease by Li, Xianting, Wang, Qing Jun, Pan, Nina, Lee, Sangkyu, Zhao, Yingming, Chait, Brian T, Yue, Zhenyu

“…Recent studies show that mutations in Leucine Rich Repeat Kinase 2 (LRRK2) are the cause of the most common inherited and some sporadic forms of Parkinson's…”
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Genome-wide gene expression tuning reveals diverse vulnerabilities of M. tuberculosis by Bosch, Barbara, DeJesus, Michael A., Poulton, Nicholas C., Zhang, Wenzhu, Engelhart, Curtis A., Zaveri, Anisha, Lavalette, Sophie, Ruecker, Nadine, Trujillo, Carolina, Wallach, Joshua B., Li, Shuqi, Ehrt, Sabine, Chait, Brian T., Schnappinger, Dirk, Rock, Jeremy M.

“…Antibacterial agents target the products of essential genes but rarely achieve complete target inhibition. Thus, the all-or-none definition of essentiality…”
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Distinct regulation of autophagic activity by Atg14L and Rubicon associated with Beclin 1-phosphatidylinositol-3-kinase complex by Wang, Qing Jun, Li, Xianting, Yan, Ying, Yue, Zhenyu, Chait, Brian T, Zhong, Yun, Heintz, Nathaniel, Backer, Jonathan M

“…Beclin 1, a mammalian autophagy protein that has been implicated in development, tumour suppression, neurodegeneration and cell death, exists in a complex with…”
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SMARCA3, a Chromatin-Remodeling Factor, Is Required for p11-Dependent Antidepressant Action by Oh, Yong-Seok, Gao, Pu, Lee, Ko-Woon, Ceglia, Ilaria, Seo, Ji-Seon, Zhang, Xiaozhu, Ahn, Jung-Hyuck, Chait, Brian T., Patel, Dinshaw J., Kim, Yong, Greengard, Paul

“…p11, through unknown mechanisms, is required for behavioral and cellular responses to selective serotonin reuptake inhibitors (SSRIs). We show that SMARCA3, a…”
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Reconstitution of active human core Mediator complex reveals a critical role of the MED14 subunit by Cevher, Murat A, Shi, Yi, Li, Dan, Chait, Brian T, Malik, Sohail, Roeder, Robert G

“…Reconstitution of a 15-subunit functional human Mediator complex establishes direct physical and functional interactions of key MED subunits with both Pol II…”
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A robust pipeline for rapid production of versatile nanobody repertoires by Fridy, Peter C, Li, Yinyin, Keegan, Sarah, Thompson, Mary K, Nudelman, Ilona, Scheid, Johannes F, Oeffinger, Marlene, Nussenzweig, Michel C, Fenyö, David, Chait, Brian T, Rout, Michael P

“…This paper presents an efficient method for generating nanobodies with high affinity and high specificity. In addition, a collection of nanobodies specific for…”
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Structural basis for backtracking by the SARS-CoV-2 replication–transcription complex by Malone, Brandon, Chen, James, Wang, Qi, Llewellyn, Eliza, Choi, Young Joo, Olinares, Paul Dominic B., Cao, Xinyun, Hernandez, Carolina, Eng, Edward T., Chait, Brian T., Shaw, David E., Landick, Robert, Darst, Seth A., Campbell, Elizabeth A.

“…Backtracking, the reverse motion of the transcriptase enzyme on the nucleic acid template, is a universal regulatory feature of transcription in cellular…”
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Comprehensive structure and functional adaptations of the yeast nuclear pore complex by Akey, Christopher W., Singh, Digvijay, Ouch, Christna, Echeverria, Ignacia, Nudelman, Ilona, Varberg, Joseph M., Yu, Zulin, Fang, Fei, Shi, Yi, Wang, Junjie, Salzberg, Daniel, Song, Kangkang, Xu, Chen, Gumbart, James C., Suslov, Sergey, Unruh, Jay, Jaspersen, Sue L., Chait, Brian T., Sali, Andrej, Fernandez-Martinez, Javier, Ludtke, Steven J., Villa, Elizabeth, Rout, Michael P.

“…Nuclear pore complexes (NPCs) mediate the nucleocytoplasmic transport of macromolecules. Here we provide a structure of the isolated yeast NPC in which the…”
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The architecture of a eukaryotic replisome by Sun, Jingchuan, Shi, Yi, Georgescu, Roxana E, Yuan, Zuanning, Chait, Brian T, Li, Huilin, O'Donnell, Michael E

“…Single-particle electron microscopy reconstruction of the budding yeast replisome locates leading-strand Pol ε and lagging-strand Pol α on opposite sides of…”
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Rif1 Prevents Resection of DNA Breaks and Promotes Immunoglobulin Class Switching by Di Virgilio, Michela, Callen, Elsa, Yamane, Arito, Zhang, Wenzhu, Jankovic, Mila, Gitlin, Alexander D., Feldhahn, Niklas, Resch, Wolfgang, Oliveira, Thiago Y., Chait, Brian T., Nussenzweig, André, Casellas, Rafael, Robbiani, Davide F., Nussenzweig, Michel C.

“…DNA double-strand breaks (DSBs) represent a threat to the genome because they can lead to the loss of genetic information and chromosome rearrangements. The…”
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