Search Results - "Chaires, Jonathan B."

G‐Quadruplex Secondary Structure Obtained from Circular Dichroism Spectroscopy by del Villar‐Guerra, Rafael, Trent, John O., Chaires, Jonathan B.

“…A curated library of circular dichroism spectra of 23 G‐quadruplexes of known structure was built and analyzed. The goal of this study was to use this…”
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Calorimetry and thermodynamics in drug design by Chaires, Jonathan B

“…Modern instrumentation for calorimetry permits direct determination of enthalpy values for binding reactions and conformational transitions in biomolecules…”
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A thermodynamic signature for drug–DNA binding mode by Chaires, Jonathan B.

“…A number of small molecules bind directly and selectively to DNA, acting as chemotherapeutic agents by inhibiting replication, transcription or topoisomerase…”
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Human telomeric G-quadruplex: thermodynamic and kinetic studies of telomeric quadruplex stability by Chaires, Jonathan B

“…Thermodynamic and kinetic studies complement high-resolution structures of G-quadruplexes. Such studies are essential for a thorough understanding of the…”
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Folding and Unfolding Pathways of the Human Telomeric G-Quadruplex by Gray, Robert D., Trent, John O., Chaires, Jonathan B.

“…Sequence analogs of human telomeric DNA such as d[AGGG(TTAGGG)3] (Tel22) fold into monomeric quadruplex structures in the presence of a suitable cation. To…”
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The hTERT core promoter forms three parallel G-quadruplexes by Monsen, Robert C, DeLeeuw, Lynn, Dean, William L, Gray, Robert D, Sabo, T Michael, Chakravarthy, Srinivas, Chaires, Jonathan B, Trent, John O

“…The structure of the 68 nt sequence with G-quadruplex forming potential within the hTERT promoter is disputed. One model features a structure with three…”
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Populated Intermediates in the Thermal Unfolding of the Human Telomeric Quadruplex by Gray, Robert D, Buscaglia, Robert, Chaires, Jonathan B

“…Thermal denaturation profiles of several model oligonucleotides of the human telomere DNA sequence including d[A(GGGTTA)3GGG] (Tel22) were determined using…”
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Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences by Monsen, Robert C, DeLeeuw, Lynn W, Dean, William L, Gray, Robert D, Chakravarthy, Srinivas, Hopkins, Jesse B, Chaires, Jonathan B, Trent, John O

“…Abstract We report on higher-order G-quadruplex structures adopted by long promoter sequences obtained by an iterative integrated structural biology approach…”
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Hydration Is a Major Determinant of the G-Quadruplex Stability and Conformation of the Human Telomere 3′ Sequence of d(AG3(TTAG3)3) by Miller, M. Clarke, Buscaglia, Robert, Chaires, Jonathan B, Lane, Andrew N, Trent, John O

“…The factors that determine the conformation and stability of G-quadruplex forming sequences remain poorly understood. Here we demonstrate the influence of…”
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Structure of a 28.5 kDa duplex-embedded G-quadruplex system resolved to 7.4 Å resolution with cryo-EM by Monsen, Robert C, Chua, Eugene Y D, Hopkins, Jesse B, Chaires, Jonathan B, Trent, John O

“…Genomic regions with high guanine content can fold into non-B form DNA four-stranded structures known as G-quadruplexes (G4s). Extensive in vivo investigations…”
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Drug discovery of small molecules targeting the higher-order hTERT promoter G-quadruplex by Monsen, Robert C, Maguire, Jon M, DeLeeuw, Lynn W, Chaires, Jonathan B, Trent, John O

“…DNA G-quadruplexes (G4s) are now widely accepted as viable targets in the pursuit of anticancer therapeutics. To date, few small molecules have been identified…”
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Structure and Stability of Higher-Order Human Telomeric Quadruplexes by Petraccone, Luigi, Spink, Charles, Trent, John O, Garbett, Nichola C, Mekmaysy, Chongkham S, Giancola, Concetta, Chaires, Jonathan B

“…G-quadruplex formation in the sequences 5′-(TTAGGG) n and 5′(TTAGGG) n TT (n = 4, 8, 12) was studied using circular dichroism, sedimentation velocity,…”
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Circular dichroism to determine binding mode and affinity of ligand-DNA interactions by Chaires, Jonathan B, Garbett, Nichola C, Ragazzon, Patricia A

“…Circular dichroism (CD) is a useful technique for an assessment of DNA-binding mode, being a more accessible, low-resolution complement to NMR and X-ray…”
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Modeling complex equilibria in isothermal titration calorimetry experiments: Thermodynamic parameters estimation for a three-binding-site model by Le, Vu H., Buscaglia, Robert, Chaires, Jonathan B., Lewis, Edwin A.

“…Isothermal titration calorimetry (ITC) is a powerful technique that can be used to estimate a complete set of thermodynamic parameters (e.g., Keq (or ΔG), ΔH,…”
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Energetics and Kinetics of a Conformational Switch in G-Quadruplex DNA by Gray, Robert D, Li, Jing, Chaires, Jonathan B

“…Circular dichroism and differential scanning calorimetry were used to determine the energetics of the conformational switch of the human telomere quadruplex…”
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POT1 stability and binding measured by fluorescence thermal shift assays by DeLeeuw, Lynn W, Monsen, Robert C, Petrauskas, Vytautas, Gray, Robert D, Baranauskiene, Lina, Matulis, Daumantas, Trent, John O, Chaires, Jonathan B

“…The protein POT1 (Protection of Telomeres 1) is an integral part of the shelterin complex that protects the ends of human chromosomes from degradation or end…”
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Activation of the Proapoptotic Bcl-2 Protein Bax by a Small Molecule Induces Tumor Cell Apoptosis by Zhao, Guoping, Zhu, Yanglong, Eno, Colins O., Liu, Yanlong, DeLeeuw, Lynn, Burlison, Joseph A., Chaires, Jonathan B., Trent, John O., Li, Chi

“…The proapoptotic Bcl-2 protein Bax by itself is sufficient to initiate apoptosis in almost all apoptotic paradigms. Thus, compounds that can facilitate…”
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Profusion of G-quadruplexes on both subunits of metazoan ribosomes by Mestre-Fos, Santi, Penev, Petar I, Richards, John Colin, Dean, William L, Gray, Robert D, Chaires, Jonathan B, Williams, Loren Dean

“…Mammalian and bird ribosomes are nearly twice the mass of prokaryotic ribosomes in part because of their extraordinarily long rRNA tentacles. Human rRNA…”
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Detection of cervical cancer biomarker patterns in blood plasma and urine by differential scanning calorimetry and mass spectrometry by Garbett, Nichola C, Merchant, Michael L, Helm, C William, Jenson, Alfred B, Klein, Jon B, Chaires, Jonathan B

“…Improved methods for the accurate identification of both the presence and severity of cervical intraepithelial neoplasia (CIN) and extent of spread of invasive…”
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Use of competition dialysis in the discovery of G-quadruplex selective ligands by Ragazzon, Patricia, Chaires, Jonathan B.

“…G-quadrplex DNA can exist in a rich variety of structural forms, ranging from unimolecular folded structures containing diverse types of loops and strand…”
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