Search Results - "Chaires, Jonathan"

G‐Quadruplex Secondary Structure Obtained from Circular Dichroism Spectroscopy by del Villar‐Guerra, Rafael, Trent, John O., Chaires, Jonathan B.

“…A curated library of circular dichroism spectra of 23 G‐quadruplexes of known structure was built and analyzed. The goal of this study was to use this…”
Get full text

Calorimetry and thermodynamics in drug design by Chaires, Jonathan B

“…Modern instrumentation for calorimetry permits direct determination of enthalpy values for binding reactions and conformational transitions in biomolecules…”
Get full text

A thermodynamic signature for drug–DNA binding mode by Chaires, Jonathan B.

“…A number of small molecules bind directly and selectively to DNA, acting as chemotherapeutic agents by inhibiting replication, transcription or topoisomerase…”
Get full text

Human telomeric G-quadruplex: thermodynamic and kinetic studies of telomeric quadruplex stability by Chaires, Jonathan B

“…Thermodynamic and kinetic studies complement high-resolution structures of G-quadruplexes. Such studies are essential for a thorough understanding of the…”
Get full text

Folding and Unfolding Pathways of the Human Telomeric G-Quadruplex by Gray, Robert D., Trent, John O., Chaires, Jonathan B.

“…Sequence analogs of human telomeric DNA such as d[AGGG(TTAGGG)3] (Tel22) fold into monomeric quadruplex structures in the presence of a suitable cation. To…”
Get full text

G‑quadruplex DNA: A Longer Story by Monsen, Robert C., Trent, John O., Chaires, Jonathan B.

“…Conspectus G-quadruplexes (G4s) are distinctive four-stranded DNA or RNA structures found within cells that are thought to play functional roles in gene…”
Get full text

Drug discovery of small molecules targeting the higher-order hTERT promoter G-quadruplex by Monsen, Robert C, Maguire, Jon M, DeLeeuw, Lynn W, Chaires, Jonathan B, Trent, John O

“…DNA G-quadruplexes (G4s) are now widely accepted as viable targets in the pursuit of anticancer therapeutics. To date, few small molecules have been identified…”
Get full text

The hTERT core promoter forms three parallel G-quadruplexes by Monsen, Robert C, DeLeeuw, Lynn, Dean, William L, Gray, Robert D, Sabo, T Michael, Chakravarthy, Srinivas, Chaires, Jonathan B, Trent, John O

“…The structure of the 68 nt sequence with G-quadruplex forming potential within the hTERT promoter is disputed. One model features a structure with three…”
Get full text

Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences by Monsen, Robert C, DeLeeuw, Lynn W, Dean, William L, Gray, Robert D, Chakravarthy, Srinivas, Hopkins, Jesse B, Chaires, Jonathan B, Trent, John O

“…Abstract We report on higher-order G-quadruplex structures adopted by long promoter sequences obtained by an iterative integrated structural biology approach…”
Get full text

Structure of a 28.5 kDa duplex-embedded G-quadruplex system resolved to 7.4 Å resolution with cryo-EM by Monsen, Robert C, Chua, Eugene Y D, Hopkins, Jesse B, Chaires, Jonathan B, Trent, John O

“…Genomic regions with high guanine content can fold into non-B form DNA four-stranded structures known as G-quadruplexes (G4s). Extensive in vivo investigations…”
Get full text

Populated Intermediates in the Thermal Unfolding of the Human Telomeric Quadruplex by Gray, Robert D, Buscaglia, Robert, Chaires, Jonathan B

“…Thermal denaturation profiles of several model oligonucleotides of the human telomere DNA sequence including d[A(GGGTTA)3GGG] (Tel22) were determined using…”
Get full text

The solution structures of higher-order human telomere G-quadruplex multimers by Monsen, Robert C, Chakravarthy, Srinivas, Dean, William L, Chaires, Jonathan B, Trent, John O

“…Abstract Human telomeres contain the repeat DNA sequence 5′-d(TTAGGG), with duplex regions that are several kilobases long terminating in a 3′ single-stranded…”
Get full text

POT1 stability and binding measured by fluorescence thermal shift assays by DeLeeuw, Lynn W, Monsen, Robert C, Petrauskas, Vytautas, Gray, Robert D, Baranauskiene, Lina, Matulis, Daumantas, Trent, John O, Chaires, Jonathan B

“…The protein POT1 (Protection of Telomeres 1) is an integral part of the shelterin complex that protects the ends of human chromosomes from degradation or end…”
Get full text

Folding Landscape of a Parallel G‑Quadruplex by Gray, Robert D, Trent, John O, Arumugam, Sengodagounder, Chaires, Jonathan B

“…Circular dichroism and stopped-flow UV spectroscopies were used to investigate the thermodynamic stability and the folding pathway of d­[TGAG3TG3TAG3TG3TA2] at…”
Get full text

Hydration Is a Major Determinant of the G-Quadruplex Stability and Conformation of the Human Telomere 3′ Sequence of d(AG3(TTAG3)3) by Miller, M. Clarke, Buscaglia, Robert, Chaires, Jonathan B, Lane, Andrew N, Trent, John O

“…The factors that determine the conformation and stability of G-quadruplex forming sequences remain poorly understood. Here we demonstrate the influence of…”
Get full text

Profusion of G-quadruplexes on both subunits of metazoan ribosomes by Mestre-Fos, Santi, Penev, Petar I, Richards, John Colin, Dean, William L, Gray, Robert D, Chaires, Jonathan B, Williams, Loren Dean

“…Mammalian and bird ribosomes are nearly twice the mass of prokaryotic ribosomes in part because of their extraordinarily long rRNA tentacles. Human rRNA…”
Get full text

Kinetics and mechanism of K⁺- and Na⁺-induced folding of models of human telomeric DNA into G-quadruplex structures by Gray, Robert D, Chaires, Jonathan B

“…Cation-induced folding into quadruplex structures for three model human telomeric oligonucleotides, d[AGGG(TTAGGG)₃], d[TTGGG(TTAGGG)₃A] and d[TTGGG(TTAGGG)₃],…”
Get full text

Strategy for modeling higher-order G-quadruplex structures recalcitrant to NMR determination by Michael Sabo, T., Trent, John O., Chaires, Jonathan B., Monsen, Robert C.

“…•NMR is the gold standard for determining the solution structures of G-quadruplexes.•Higher-order G-quadruplexes (xG4s) are often not amenable to…”
Get full text

Detection of cervical cancer biomarker patterns in blood plasma and urine by differential scanning calorimetry and mass spectrometry by Garbett, Nichola C, Merchant, Michael L, Helm, C William, Jenson, Alfred B, Klein, Jon B, Chaires, Jonathan B

“…Improved methods for the accurate identification of both the presence and severity of cervical intraepithelial neoplasia (CIN) and extent of spread of invasive…”
Get full text

A small molecule-DNA binding landscape by Chaires, Jonathan B.

“…ABSTRACT This brief account traces the development of a “competition dialysis” method used to characterize the structural and sequence selectivity of DNA…”
Get full text