Optimization of Submerged Culture Parameters of the Aphid Pathogenic Fungus Fusarium equiseti Based on Sporulation and Mycelial Biomass

Optimization of Submerged Culture Parameters of the Aphid Pathogenic Fungus Fusarium equiseti Based on Sporulation and Mycelial Biomass

(JMF-01), as an entomopathogenic fungus, can effectively control agricultural pests and has the potential to be a biocontrol agent. To promote mycelial growth and sporulation, we investigated the optimal submerged culture conditions for . In this study, we used the single-factor method and Box-Behnk...

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Bibliographic Details
Published in:Microorganisms (Basel) Vol. 11; no. 1; p. 190
Main Authors: Zhao, Xueyi, Chai, Junfa, Wang, Fang, Jia, Yanxia
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 12-01-2023
MDPI
Subjects:
Biological control
Biomass
box–behnken design
Cadavers
Conidia
Density
Entomopathogenic fungi
Experiments
Fungi
Fusarium
Fusarium equiseti
Inoculum
Insects
Laboratories
Mycelia
Myzus persicae
optimal submerged culture conditions
Optimization
Pest control
Pesticides
Pests
pH effects
response surface methodology
Sporulation
Virulence
Fusarium equiseti
response surface methodology
optimal submerged culture conditions
box–behnken design
Myzus persicae
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Summary:(JMF-01), as an entomopathogenic fungus, can effectively control agricultural pests and has the potential to be a biocontrol agent. To promote mycelial growth and sporulation, we investigated the optimal submerged culture conditions for . In this study, we used the single-factor method and Box-Behnken design and determined the virulence of the submerged culture against after optimization. As a result, the highly significant factors affecting the spore concentration of strain JMF-01 were the primary inoculum density and the initial pH, and the highly significant factor affecting the mycelial biomass was the medium-to-flask ratio. The highest mycelial biomass value was 0.35 g when the incubation time was 5.68 days, the initial pH was 5.11, the medium-to-flask ratio was 0.43, and 1 mL of the primary inoculum with spore density of 0.97 × 10 conidia/mL was added. When the incubation time was 6.32 days, the initial pH was 4.46, the medium-to-flask ratio was 0.35, the primary inoculum density was 1.32 × 10 conidia/mL of 1 mL, and the highest spore concentration of 6.49 × 10 blastospores/mL was obtained. Compared with the unoptimized medium conditions, the optimized submerged culture had the highest mycelial biomass and spore concentration, which were 3.46 and 2.06 times higher, respectively. The optimized submerged culture was highly pathogenic toward , reaching a 95% mortality rate. Our results provide optimal submerged culture conditions for and lay the basis for later research to expand production for pest control.
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ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms11010190