Molecular diversity and abundance of the microbial community associated to an offshore oil field on the southeast of Brazil

Molecular diversity and abundance of the microbial community associated to an offshore oil field on the southeast of Brazil

Petroleum microbiology is important to the fossil oil industry and any related environmental research. Assessment of resident microbial communities is essential for controlling many technical, operational, and environmental issues. However, difficulties of sampling and extraction are limiting factor...

Full description

Saved in:
Bibliographic Details
Published in:International biodeterioration & biodegradation Vol. 160; p. 105215
Main Authors: de Sousa Pires, Araceli, Dias, Graciela Maria, Chagas de Oliveira Mariano, Danielly, Akamine, Rubens Nobumoto, Cruz de Albuquerque, Ana Carla, Groposo, Cláudia, Soares Ribeiro, Cláudia Maria, de Figueiredo Vilela, Leonardo, Neves, Bianca Cruz
Format: Journal Article
Language:English
Published: Barking Elsevier Ltd 01-05-2021
Elsevier BV
Subjects:
16S rRNA gene
Abundance
Archaea
Bacteria
Bacterial corrosion
Campos basin
Complexity
Corrosion products
Desulfobacterota
DPANN superphylum Archaea
Environmental assessment
Environmental research
Food chains
Methanogenic bacteria
Microbial activity
Microbiology
Microbiomes
Microorganisms
Oil and gas fields
Oil and gas industry
Oil fields
Oil reservoirs
Petroleum industry
Relative abundance
rRNA 16S
Sulfate reduction
Sulfate-reducing microorganisms
Brazil
Sulfate-reducing microorganisms
Desulfobacterota
DPANN superphylum Archaea
Campos basin
16S rRNA gene
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Petroleum microbiology is important to the fossil oil industry and any related environmental research. Assessment of resident microbial communities is essential for controlling many technical, operational, and environmental issues. However, difficulties of sampling and extraction are limiting factors. Herein, we assessed the bacterial and archaeal population of sulphate-reducing microorganisms (SRM) in a production water from an industrial setting with corrosion signals, within a Brazilian offshore oil reservoir. Four hypervariable regions of 16S rRNA gene were sequenced, while dsrA gene was quantified by RT-qPCR to estimate the SRM abundance. Results showed that richness and diversity of were greater in bacteria than archaea population, revealing a domination of Desulfobacterota and Nanoarchaeota phyla, respectively. RT-qPCR analyses indicated that dsrA reached 2.65 × 103 copies.ml−1, which is consistent with the relative abundance of bacteria/archaea methanogens detected by 16S analyses. The content of methanogen archaea suggested a relatively low abundance of these organisms. Despite the strategic and economical importance of the Campos Basin, little is known about the complexity of the microbial communities present in its oil field food chain, and our results have expanded the knowledge of that environment, highlighting its complexity. •Composition of a microbial community in the production water from an oil field.•Abundance of sulphate-reducing microorganisms in the production water.•Bacterial population was dominated by Desulfobacterota, including sulphate-reducing bacteria.•Archaeal population was dominated by the newly proposed DPANN superphylum archaea.
ISSN:0964-8305
1879-0208
DOI:10.1016/j.ibiod.2021.105215