Assessment of free fetal DNA concentration in maternal plasma during the first trimester of pregnancy: comparative study between EDTA and PPT tubes - pilot study

Abstract Objective: To compare ethylenediamine tetraacetic acid (EDTA) tubes and plasma preparation tubes (PPT) for evaluating maternal plasma during the first trimester of pregnancy. Methods: A cross-sectional study was conducted on 24 male fetuses in women between 6 and 14 weeks of pregnancy. Bloo...

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Published in:	The journal of maternal-fetal & neonatal medicine Vol. 28; no. 2; pp. 172 - 176
Main Authors:	Chadud, Carolina Schneider, Araujo Júnior, Edward, Martinhago, Ciro Dresh, Andari, Viviane Cristina Mello, Tedesco, Giselle Darahem, Bussamra, Luiz Claudio Silva, Aoki, Tsutomu
Format:	Journal Article
Language:	English
Published:	England Informa UK Ltd 01-01-2015 Informa Healthcare
Subjects:	Anticoagulants - pharmacology Blood Preservation - methods Blood Specimen Collection - methods Cell Cycle Proteins - analysis Cell Cycle Proteins - genetics Cross-Sectional Studies DNA - analysis DNA - blood Edetic Acid - pharmacology EDTA Female Fetus - metabolism first trimester of pregnancy free fetal DNA Gestational Age Humans Male PCR Pilot Projects Polymerase Chain Reaction PPT Pregnancy Pregnancy Trimester, First - blood Pregnancy Trimester, First - genetics free fetal DNA EDTA PPT first trimester of pregnancy PCR
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Summary:	Abstract Objective: To compare ethylenediamine tetraacetic acid (EDTA) tubes and plasma preparation tubes (PPT) for evaluating maternal plasma during the first trimester of pregnancy. Methods: A cross-sectional study was conducted on 24 male fetuses in women between 6 and 14 weeks of pregnancy. Blood samples (10 mL) were collected and stored in EDTA and PPT tubes. Subsequently, the samples were centrifuged and sent for free fetal DNA extraction by means of the polymerase chain reaction (PCR) technique. The reactions were performed in a real time PCR machine for detecting the amplification products. The genome region chosen for performing the PCR reactions was a target specific for the Y chromosome, in which the DYS-14 marker was amplified only when the DNA was of male sex. The free fetal DNA concentration was given by the threshold cycle (TC). To compare the tubes, the paired Student t-test was used. Results: The mean gestational age was 11.08 ± 2.30 weeks (range: 6-14). The mean TC for PPT was 30.08 ± 1.05 (range: 27.08-32.61) and for EDTA, 30.23 ± 0.96 (range: 28.01-32.09), but without statistical significance (p = 0.357). Conclusion: We did not observe any statistically significant difference in free fetal DNA concentration between the EDTA and PPT tubes.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1476-7058 1476-4954
DOI:	10.3109/14767058.2014.908843