In Vitro Susceptibility Characteristics of Cryptococcus neoformans Varieties from AIDS Patients in Goiânia, Brazil

Sixty clinical isolates of Cryptococcus neoformans from AIDS from Goiânia, state of Goiás, Brazil, were characterized according to varieties, serotypes and tested for antifungal susceptibility. To differentiate the two varieties was used L-canavanine-glycine-bromothymol blue medium and to separate t...

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Published in:	Memórias do Instituto Oswaldo Cruz Vol. 98; no. 6; pp. 839 - 841
Main Authors:	de F L Fernandes, Orionalda, Passos, Xisto S, Souza, Lúcia K H, Miranda, André T B, Cerqueira, Carlos Henrique P V, Silva, Maria do Rosário R
Format:	Journal Article
Language:	English
Published:	Brazil Fundação Oswaldo Cruz, Fiocruz 01-09-2003 Instituto Oswaldo Cruz, Ministério da Saúde Fundação Oswaldo Cruz (FIOCRUZ)
Subjects:	Adolescent Adult Agglutination Tests AIDS-Related Opportunistic Infections - microbiology Amphotericin B - pharmacology Antifungal Agents - pharmacology antifungal susceptibility Brazil Cryptococcus neoformans Cryptococcus neoformans - classification Cryptococcus neoformans - drug effects Cryptococcus neoformans - isolation & purification Female Fluconazole - pharmacology Goiânia Humans Itraconazole - pharmacology Male Microbial Sensitivity Tests Middle Aged PARASITOLOGY serotypes serotypes - antifungal susceptibility - Cryptococcus neoformans - Goiânia - Brazil Serotyping TROPICAL MEDICINE Brazil, Goiania Brazil Goiânia serotypes Cryptococcus neoformans antifungal susceptibility
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Summary:	Sixty clinical isolates of Cryptococcus neoformans from AIDS from Goiânia, state of Goiás, Brazil, were characterized according to varieties, serotypes and tested for antifungal susceptibility. To differentiate the two varieties was used L-canavanine-glycine-bromothymol blue medium and to separate the serotypes was used slide agglutination test with Crypto Check Iatron. The Minimal Inhibitory Concentration (MIC) of fluconazole, itraconazole, and amphotericin B were determined by the National Committee for Clinical Laboratory Standards macrodilution method. Our results identified 56 isolates as C. neoformans var. neoformans serotype A and 4 isolates as C. neoformans var. gattii serotype B. MIC values for C. neoformans var. gattii were higher than C. neoformans var. neoformans. We verified that none isolate was resistant to itraconazole and to amphotericin B, but one C. neoformans var. neoformans and three C. neoformans var. gattii isolates were resistant to fluconazole. The presence of C. neoformans var. gattii fluconazole resistant indicates the importance of determining not only the variety of C. neoformans infecting the patients but also measuring the MIC of the isolate in order to properly orient treatment.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	1678-8060 0074-0276 0074-0276 1678-8060
DOI:	10.1590/S0074-02762003000600022