A novel approach for identification of biologically active phenolic compounds in complex matrices using hybrid quadrupole-orbitrap mass spectrometer: A promising tool for testing antimicrobial activity of hops

The phenolic compounds, secondary metabolites of hops represent a large family of compounds that could be subsequently divided into smaller groups based on the similarities between their chemical structures. The antibacterial, antifungal and antiviral properties of hops are well known, but there is...

Full description

Saved in:

Bibliographic Details
Published in:	Talanta (Oxford) Vol. 156-157; pp. 209 - 217
Main Authors:	Duseka, Martin, Jandovskaa, Vladimira, Cermakb, Pavel, Mikyskaa, Alexandr, Olsovskaa, Jana
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 15-08-2016
Subjects:	Anti-Infective Agents - analysis Anti-Infective Agents - isolation & purification Anti-Infective Agents - pharmacology Antibacterial properties Antiinfectives and antibacterials Antimicrobial activity Chemical Fractionation - methods Cones Extraction Fragmentation High-resolution mass spectrometry Hops Humans Humulus - chemistry Humulus - growth & development Inhibition Lists Phenols Phenols - analysis Phenols - isolation & purification Phenols - pharmacology QuEChERS Staphylococcal Infections - drug therapy Staphylococcus aureus Staphylococcus aureus - drug effects Tandem Mass Spectrometry - methods Hops Antimicrobial activity High-resolution mass spectrometry Extraction QuEChERS
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The phenolic compounds, secondary metabolites of hops represent a large family of compounds that could be subsequently divided into smaller groups based on the similarities between their chemical structures. The antibacterial, antifungal and antiviral properties of hops are well known, but there is a lack of information about antimicrobial activities of individual hop compounds. This study was carried out with an objective to identify compounds present in hops that have potential antibacterial activity. In the first stage of experiment, the active compounds with potential anti-microbial activity had to be extracted from hop cones. Therefore, minced hop cones were applied on solid growth medium inoculated with Staphylococcus aureus. The active substances that migrated into the medium created an inhibition zone. In the second stage of experiment, the inhibition zones were cut out from Petri dishes, active compounds were extracted from these zones and consequently analyzed using LC-HRMS. These complex assays were developed and optimized. The data were acquired by using a quadrupole-orbitrap hybrid mass spectrometer by targeted-MS2 experiment in both ionization modes. The MS method has been developed as a screening method with a subsequent fragmentation of compound of interest on the base of inclusion mass list. The unknown compounds extracted from inhibition zones have been identified either by searching against a database or their structure has been elucidated on the basis of their fragmentation spectra. On the basis of this experiment the list of active compounds with potential anti-microbial activities was enhanced. [Display omitted] •A procedure for extraction of phenolic compounds from solid-growth medium was newly developed.•Potentially anti-microbial active compounds from hop were identified by LC-HR/MS/MS.•Pre-selection of active compounds using their ability to form the inhibition zone was proved.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0039-9140 1873-3573
DOI:	10.1016/j.talanta.2016.05.018