Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk-based extender

Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk-based extender

•Ram semen collection and cryopreservation.•Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on Ram semen quality.•Lecithin and egg yolk extender for ram semen cryopreservation.•Defected acrosome and plasma membrane integrity were evaluation. The aim of the current study was to evaluate...

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Published in:Animal reproduction science Vol. 164; pp. 97 - 104
Main Authors: Ustuner, Burcu, Alcay, Selim, Toker, M. Berk, Nur, Zekariya, Gokce, Elif, Sonat, Fusun Ak, Gul, Zulfiye, Duman, Muhammed, Ceniz, Cafer, Uslu, Aydın, Sagirkaya, Hakan, Soylu, M. Kemal
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-01-2016
Subjects:
Acrosome
Animals
Cell Membrane
Cryopreservation
Cryopreservation - veterinary
Cryoprotective Agents
Egg Yolk
Male
Malondialdehyde
Oncorhynchus mykiss
Plant Lectins
Rainbow trout
Ram semen
Semen - physiology
Semen Preservation - veterinary
Seminal plasma
Sheep
Soybean Proteins
Ram semen
Seminal plasma
Cryopreservation
Rainbow trout
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Summary:•Ram semen collection and cryopreservation.•Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on Ram semen quality.•Lecithin and egg yolk extender for ram semen cryopreservation.•Defected acrosome and plasma membrane integrity were evaluation. The aim of the current study was to evaluate the effects of different concentrations of rainbow trout seminal plasma (RTSP) (0.1%, 1% and 10%) in extenders containing either egg yolk or lecithin for use in Awassi ram semen cryopreservation. Pooled sperm were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in egg yolk or lecithin extender containing no RTSP, 0.1%, 1% or 10% RTSP (v/v). Semen samples were assessed for sperm motility, plasma membrane integrity [hypoosmotic swelling test (HOST) and Hoechst 33258] and defective acrosomes [FITC-conjugated Pisum sativum agglutinin (PSA-FITC)] at the following five time points: after dilution with extender A; after equilibration; and post-thaw at 0h, 3h and 5h. Malondialdehyde (MDA) was examined only after thawing. Freezing and thawing procedures (dilution, equilibration and post-thaw incubation at 0h, 3h and 5h) negatively affected the motility (P<0.001) and acrosome integrity (P<0.001). Additionally, freezing and thawing negatively affected the plasma membrane integrity, as determined by the HOST and Hoechst 33258 (P<0.001). The extender group affected the motility (P<0.001) and the HOST results (P<0.001). Levels of MDA in the egg yolk extender with 1% RTSP group were significantly lower than in the lecithin control group (P<0.05). In conclusion, the egg yolk extender groups that were supplemented with 10% and 1% RTSP provided greater cryoprotective effects for semen survivability during 5h incubation than the other extender groups.
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ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2015.11.017