Search Results - "Cecchini, Davide A"

Functional metagenomics reveals novel pathways of prebiotic breakdown by human gut bacteria by Cecchini, Davide A, Laville, Elisabeth, Laguerre, Sandrine, Robe, Patrick, Leclerc, Marion, Doré, Joël, Henrissat, Bernard, Remaud-Siméon, Magali, Monsan, Pierre, Potocki-Véronèse, Gabrielle

“…The human intestine hosts a complex bacterial community that plays a major role in nutrition and in maintaining human health. A functional metagenomic approach…”
Get full text

Role of Glycoside Phosphorylases in Mannose Foraging by Human Gut Bacteria by Ladevèze, Simon, Tarquis, Laurence, Cecchini, Davide A., Bercovici, Juliette, André, Isabelle, Topham, Christopher M., Morel, Sandrine, Laville, Elisabeth, Monsan, Pierre, Lombard, Vincent, Henrissat, Bernard, Potocki-Véronèse, Gabrielle

“…To metabolize both dietary fiber constituent carbohydrates and host glycans lining the intestinal epithelium, gut bacteria produce a wide range of…”
Get full text

A comparison between immobilized pyrimidine nucleoside phosphorylase from Bacillus subtilis and thymidine phosphorylase from Escherichia coli in the synthesis of 5-substituted pyrimidine 2′-deoxyribonucleosides by Serra, Immacolata, Bavaro, Teodora, Cecchini, Davide A., Daly, Simona, Albertini, Alessandra M., Terreni, Marco, Ubiali, Daniela

“…•Immobilized PyNP from B. subtilis and TP from E. coli catalyze the synthesis of 5-halogenated pyrimidine 2′-deoxynucleosides.•Differences between the enzymes…”
Get full text

New active site oriented glyoxyl-agarose derivatives of Escherichia coli penicillin G acylase by Cecchini, Davide A, Serra, Immacolata, Ubiali, Daniela, Terreni, Marco, Albertini, Alessandra M

“…Immobilized Penicillin G Acylase (PGA) derivatives are biocatalysts that are industrially used for the hydrolysis of Penicillin G by fermentation and for the…”
Get full text

Biochemical identification of the catalytic residues of a glycoside hydrolase family 120 β-xylosidase, involved in xylooligosaccharide metabolisation by gut bacteria by Cecchini, Davide A., Fauré, Régis, Laville, Elisabeth, Potocki-Veronese, Gabrielle

“…•The GH120 BaXylB from Bifidobacterium adolescentis is a retaining β-xylosidase.•Asp393 and Glu416 act as nucleophile and acid/base catalyst…”
Get full text

Efficient biocatalyst for large-scale synthesis of cephalosporins, obtained by combining immobilization and site-directed mutagenesis of penicillin acylase by Cecchini, Davide A., Pavesi, Roberto, Sanna, Sara, Daly, Simona, Xaiz, Roberto, Pregnolato, Massimo, Terreni, Marco

“…We describe the rational design of a new efficient biocatalyst and the development of a sustainable green process for the synthesis of cephalosporins bearing a…”
Get full text

Functional Metagenomics: Construction and High-Throughput Screening of Fosmid Libraries for Discovery of Novel Carbohydrate-Active Enzymes by Ufarté, Lisa, Bozonnet, Sophie, Laville, Elisabeth, Cecchini, Davide A, Pizzut-Serin, Sandra, Jacquiod, Samuel, Demanèche, Sandrine, Simonet, Pascal, Franqueville, Laure, Veronese, Gabrielle Potocki

“…Activity-based metagenomics is one of the most efficient approaches to boost the discovery of novel biocatalysts from the huge reservoir of uncultivated…”
Get more information

Assessment of immobilized PGA orientation via the LC-MS analysis of tryptic digests of the wild type and its 3K-PGA mutant assists in the rational design of a high-performance biocatalyst by Serra, Immacolata, Ubiali, Daniela, Cecchini, Davide A., Calleri, Enrica, Albertini, Alessandra M., Terreni, Marco, Temporini, Caterina

“…The mutant penicillin G acylase (PGA) 3K-PGA contains three additional Lys residues on the surface opposite the active site. This protein was designed to…”
Get full text

Coupling of Site-Directed Mutagenesis and Immobilization for the Rational Design of More Efficient Biocatalysts: The Case of Immobilized 3G3K PGA from E. coli by Serra, Immacolata, Cecchini, Davide A., Ubiali, Daniela, Manazza, Elena M., Albertini, Alessandra M., Terreni, Marco

“…We have investigated the synthetic performance of the immobilized 3G3K mutant of the Penicillin G acylase (PGA) from E. coli obtained by site‐directed…”
Get full text

Functional Metagenomics Reveals Novel Pathways of Prebiotic Breakdown by Human Gut Bacteria: e72766 by Cecchini, Davide A, Laville, Elisabeth, Laguerre, Sandrine, Robe, Patrick, Leclerc, Marion, Dore, Joeel, Henrissat, Bernard, Remaud-Simeon, Magali, Monsan, Pierre, Potocki-Veronese, Gabrielle

“…The human intestine hosts a complex bacterial community that plays a major role in nutrition and in maintaining human health. A functional metagenomic approach…”
Get full text

New active site oriented glyoxyl-agarose derivatives of Escherichia colipenicillin G acylase by Cecchini, Davide A, Serra, Immacolata, Ubiali, Daniela, Terreni, Marco, Albertini, Alessandra M

Get full text