Genotoxicity of PM2.5 and PM1.0 Particulates on Human Peripheral Blood Lymphocytes in Manila, Philippines

Genotoxicity of PM2.5 and PM1.0 Particulates on Human Peripheral Blood Lymphocytes in Manila, Philippines

Urban air quality is increasingly being studied as a fraction of the world’s population is living in megacities. In this study, particulate matter (PM) along Taft Avenue, Manila, the Philippines, is investigated in terms of its ability to induce genetic damage in human peripheral blood lymphocytes (...

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Bibliographic Details
Published in:Atmosphere Vol. 13; no. 1; p. 6
Main Authors: Estonilo, Ma. Katrina Gale, Cazeñas, Joedith Anne, Villafuerte, Carlos Josef, Deocaris, Custer, Caraos, Gloriamaris, Robles, Gerardo Jose, Galvez, Maria Cecilia, Asaad, Celia, Vallar, Edgar
Format: Journal Article
Language:English
Published: Basel MDPI AG 01-01-2022
Subjects:
Absorption spectroscopy
Air
Air pollution
Air quality
Air sampling
Analytical methods
Atomic absorption spectroscopy
Atomic properties
Blood
Cells
Chromosome aberrations
Cytokinesis
cytokinesis-block proliferation index
DNA methylation
Environmental policy
Genotoxicity
in vitro micronucleus test
Lung cancer
Lymphocytes
Manganese
Megacities
Outdoor air quality
Particulate emissions
Particulate matter
Particulates
Peripheral blood
Pollutants
Potassium
Proliferation
Roadsides
Spectral analysis
Spectroscopy
Suspended particulate matter
Urban air
Urban air quality
Variance analysis
Zinc
Philippines
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Summary:Urban air quality is increasingly being studied as a fraction of the world’s population is living in megacities. In this study, particulate matter (PM) along Taft Avenue, Manila, the Philippines, is investigated in terms of its ability to induce genetic damage in human peripheral blood lymphocytes (PBLs). Size-segregated roadside air samples were obtained from 2015–2017 near a university gate and analyzed using in vitro micronucleus (MN) and cytokinesis-block proliferation tests. While cellular proliferation was unaffected by 0–0.1 kg/m3 of PM1.0 and PM2.5, PBL cells treated with PM2.5 displayed a significantly higher micronucleus count (p = 0.03) compared to the cells treated with PM1.0. Atomic absorption spectroscopy revealed greater amounts of Cd, Ca, Pb, K, Na, and Zn in PM2.5 compared to PM1.0. The results indicate that the differences in composition of the two size fractions of air particulates are associated with their genotoxicities.
ISSN:2073-4433
2073-4433
DOI:10.3390/atmos13010006