Dynamic Disruptions in Nuclear Envelope Architecture and Integrity Induced by HIV-1 Vpr

Human immunodeficiency virus-1 (HIV-1) Vpr expression halts the proliferation of human cells at or near the G2cell-cycle checkpoint. The transition from G2to mitosis is normally controlled by changes in the state of phosphorylation and subcellular compartmentalization of key cell-cycle regulatory pr...

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Bibliographic Details
Published in:	Science (American Association for the Advancement of Science) Vol. 294; no. 5544; pp. 1105 - 1108
Main Authors:	Carlos M. C. de Noronha, Sherman, Michael P., Lin, Harrison W., Cavrois, Marielle V., Moir, Robert D., Goldman, Robert D., Greene, Warner C.
Format:	Journal Article
Language:	English
Published:	Washington, DC American Society for the Advancement of Science 02-11-2001 American Association for the Advancement of Science The American Association for the Advancement of Science
Subjects:	Active Transport, Cell Nucleus Biological and medical sciences cdc25 Phosphatases - metabolism Cell Cycle Proteins - metabolism Cell nucleus Cell Nucleus - metabolism Cell Nucleus - virology Cell research Cellular biology Cyclin B - metabolism Cyclin B1 Cyclins Cytological research Cytoplasm - metabolism Daughter cells Fundamental and applied biological sciences. Psychology G2 Phase Gene Products, vpr - genetics Gene Products, vpr - physiology HeLa Cells HIV HIV (Viruses) HIV 1 HIV-1 - physiology Human immunodeficiency virus Human immunodeficiency virus 1 Humans Interphase Lamin Type B Lamins Macrophages - virology Microbiology Microscopy, Fluorescence Microscopy, Video Mitosis Molecular biology Mutation Nuclear Envelope - metabolism Nuclear Envelope - ultrastructure Nuclear lamina Nuclear Pore Complex Proteins - metabolism Nuclear Proteins - metabolism Protein-Tyrosine Kinases - metabolism Proteins Recombinant Fusion Proteins - metabolism Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Transfection United States Virology Virus Integration vpr Gene Products, Human Immunodeficiency Virus vpr protein United States Virus Growing phase Cell culture HIV-1 virus Mitosis Retroviridae Cell division G2 Phase Human immunodeficiency virus Lentivirus Host virus relation
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Summary:	Human immunodeficiency virus-1 (HIV-1) Vpr expression halts the proliferation of human cells at or near the G2cell-cycle checkpoint. The transition from G2to mitosis is normally controlled by changes in the state of phosphorylation and subcellular compartmentalization of key cell-cycle regulatory proteins. In studies of the intracellular trafficking of these regulators, we unexpectedly found that wild-type Vpr, but not Vpr mutants impaired for G2arrest, induced transient, localized herniations in the nuclear envelope (NE). These herniations were associated with defects in the nuclear lamina. Intermittently, these herniations ruptured, resulting in the mixing of nuclear and cytoplasmic components. These Vpr-induced NE changes probably contribute to the observed cell-cycle arrest.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.1063957