The expression of CPEB proteins is sequentially regulated during zebrafish oogenesis and embryogenesis

SUMMARY In many species, there is little transcription in the mature oocyte, and zygotic transcription does not begin immediately after fertilization. In zebrafish, zygotic transcription is not initiated until the mid‐blastula transition, thus the production of new proteins during oogenesis and earl...

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Published in:	Molecular reproduction and development Vol. 81; no. 4; pp. 376 - 387
Main Authors:	O'Connell, Marcia L., Cavallo Jr, William C., Firnberg, Maytal
Format:	Journal Article
Language:	English
Published:	United States Blackwell Publishing Ltd 01-04-2014 Wiley Subscription Services, Inc
Subjects:	3' Untranslated Regions Animals Blastula - metabolism Body Patterning - genetics Cytoplasm - metabolism Danio rerio Drosophila ELAV Proteins - biosynthesis ELAV Proteins - genetics Embryo, Nonmammalian - metabolism Female Freshwater Gene Expression Regulation, Developmental Heterogeneous-Nuclear Ribonucleoprotein Group A-B - genetics mRNA Cleavage and Polyadenylation Factors - biosynthesis mRNA Cleavage and Polyadenylation Factors - genetics mRNA Cleavage and Polyadenylation Factors - physiology Oocytes - metabolism Oogenesis - genetics Polyadenylation Protein Biosynthesis RNA Processing, Post-Transcriptional RNA, Messenger - biosynthesis RNA, Messenger - genetics RNA-Binding Proteins - genetics RNA-Binding Proteins - physiology Zebrafish - embryology Zebrafish - genetics Zebrafish - physiology Zebrafish Proteins - biosynthesis Zebrafish Proteins - genetics Zebrafish Proteins - physiology
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Summary:	SUMMARY In many species, there is little transcription in the mature oocyte, and zygotic transcription does not begin immediately after fertilization. In zebrafish, zygotic transcription is not initiated until the mid‐blastula transition, thus the production of new proteins during oogenesis and early embryogenesis is dependent on the translation of maternal mRNAs. In a growing number of species, the translation of key maternal transcripts is coupled to their cytoplasmic polyadenylation. One family of RNA‐binding proteins implicated in this process is the cytoplasmic polyadenylation element (CPE)‐binding proteins (CPEBs), which bind to a sequence in the 3′‐untranslated regions of regulated transcripts and mediate their storage/repression or translation. In several species, there is evidence for two classes of CPEBs, a larger oocyte‐type and a smaller CPEB that functions during embryogenesis. This appears to be the case in zebrafish as well, and we now provide evidence suggesting that the oocyte‐type CPEB (zorba) regulates the translation of the embryonic‐type (ElrA) by keeping the ElrA transcript in storage until fertilization. When zorba levels fall, ElrA protein is then produced and available to regulate the translation of additional mRNAs during embryogenesis. We have also identified a potential target of ElrA, the maternal mRNA for hnRNPab, which is a potential homolog of the Drosophila gene squid, whose product plays a role in patterning the Drosophila oocyte and embryo. These data suggest that during zebrafish embryogenesis, cytoplasmic polyadenylation mediates a cascade of translational control whose final targets play central patterning roles during embryogenesis. Mol. Reprod. Dev. 81: 376–387, 2014. © 2014 Wiley Periodicals, Inc.
Bibliography:	istex:CDC9AF6174F5C4EE9649609483EED8470DA7271F ark:/67375/WNG-L3482ZN2-2 ArticleID:MRD22306 NSF - No. IBN-9707479 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1040-452X 1098-2795
DOI:	10.1002/mrd.22306