Resistance training prevents dynamics and mitochondrial respiratory dysfunction in vastus lateralis muscle of ovariectomized rats

To investigate whether ovariectomy affects mitochondrial respiratory function, gene expression of the biogenesis markers and mitochondrial dynamics of the vastus lateralis muscle, female Wistar rats divided into ovariectomized (OVX) and intact (INT) groups were kept sedentary (SED) or submitted to r...

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Published in:	Experimental gerontology Vol. 173; p. 112081
Main Authors:	Marin, Cecília Tardivo, de Souza Lino, Anderson Diogo, Avelar, Iara Dinik Santos, Barbosa, Marina Rodrigues, Scarlato, Gabriel Carlos Goulart, Cavalini, Diego Felipe, Tamanini, Fábio, Alexandrino, André Vessoni, Vercesi, Anibal Eugênio, Shiguemoto, Gilberto Eiji
Format:	Journal Article
Language:	English
Published:	England Elsevier Inc 01-03-2023 Elsevier
Subjects:	Animals Female Mitochondria - pathology Mitochondria - physiology Mitochondrial dynamics Mitochondrial dysfunction Ovariectomy Ovariectomy - adverse effects OXPHOS Physical Conditioning, Animal - physiology Quadriceps Muscle - pathology Quadriceps Muscle - physiology Rats Rats, Wistar Resistance Training OXPHOS Mitochondrial dysfunction Ovariectomy Resistance training Mitochondrial dynamics
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Summary:	To investigate whether ovariectomy affects mitochondrial respiratory function, gene expression of the biogenesis markers and mitochondrial dynamics of the vastus lateralis muscle, female Wistar rats divided into ovariectomized (OVX) and intact (INT) groups were kept sedentary (SED) or submitted to resistance training (RT) performed for thirteen weeks on a vertical ladder in which animals climbed with a workload apparatus. RT sessions were performed with four climbs with 65, 85, 95, and 100 % of the rat's previous maximum workload. Mitochondrial Respiratory Function data were obtained by High-resolution respirometry. Gene expression of FIS1, MFN1 and PGC1-α was evaluated by real-time PCR. There was a decrease on oxidative phosphorylation capacity in OVX-SED compared to other groups. Trained groups presented increase on oxidative phosphorylation capacity when compared to sedentary groups. For respiratory control ratio (RCR), OVX-SED presented lower values when compared to INT-SED and to trained groups. Trained groups presented RCR values higher compared to INT-SED. Exercise increased the values of FIS1, MFN1 and PGC1-α expression compared to OVX-SED. Our results demonstrated that in the absence of ovarian hormones, there is a great decrease in oxidative phosphorylation and electron transfer system capacities of sedentary animals. RT was able to increase the expression of genes related to mitochondrial dynamics markers, reversing the condition determined by ovariectomy.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0531-5565 1873-6815
DOI:	10.1016/j.exger.2023.112081