Detection of human herpesvirus 6 and 7 DNA in saliva from healthy adults from Rio de Janeiro, Brazil

In this study, we aimed to evaluate virus shedding in the saliva of healthy adults from the metropolitan region of the city of Rio de Janeiro, Brazil, in order to verify the prevalence of both human herpesviruses 6 and 7 (HHV-6, HHV-7). The studied group comprised 182 healthy individuals at Pedro Er...

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Bibliographic Details
Published in:	Memórias do Instituto Oswaldo Cruz Vol. 105; no. 7; pp. 925 - 927
Main Authors:	Magalhães, Ivna M, Martins, Rebeca V.N, Cossatis, João J, Cavaliere, Renata M, Afonso, Larissa A, Moysés, Natalia, Oliveira, Solange A, Cavalcanti, Silvia M.B
Format:	Journal Article
Language:	English
Published:	Brazil Fundação Oswaldo Cruz, Fiocruz 01-11-2010 Instituto Oswaldo Cruz, Ministério da Saúde Fundação Oswaldo Cruz (FIOCRUZ)
Subjects:	Adult Brazil - epidemiology DNA, Viral - analysis Female healthy adults Herpesvirus 6, Human - genetics Herpesvirus 6, Human - isolation & purification Herpesvirus 7, Human - genetics Herpesvirus 7, Human - isolation & purification Human herpesvirus 6 Human herpesvirus 6B Human herpesvirus 7 Humans Male PARASITOLOGY Polymerase Chain Reaction Prevalence Roseolovirus Roseolovirus Infections - diagnosis Roseolovirus Infections - epidemiology Roseolovirus- healthy adults - saliva saliva Saliva - chemistry Saliva - virology TROPICAL MEDICINE Virus Shedding Brazil saliva Roseolovirus healthy adults
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Summary:	In this study, we aimed to evaluate virus shedding in the saliva of healthy adults from the metropolitan region of the city of Rio de Janeiro, Brazil, in order to verify the prevalence of both human herpesviruses 6 and 7 (HHV-6, HHV-7). The studied group comprised 182 healthy individuals at Pedro Ernesto University Hospital, who were being seen for annual odontologic revisions. Saliva specimens were subjected to a multiplex polymerase chain reaction (PCR) to detect the presence of HHV-6A, HHV-6B and HHV-7. The total Roseolovirus DNA prevalence was 22.4%. The PCR detected a HHV-6 prevalence of 9.8%, with HHV-6A detected in 7.1% of the samples and HHV-6B in 2.7%. HHV-7 DNA was revealed in 12.6% of the studied cases. Multiple infections caused by HHV-6A and 7 were found in 2.1% of the samples. No statistical differences were observed regarding age, but for HHV-7 infection, an upward trend was observed in female patients. Compared to studies from other countries, low prevalence rates of herpesvirus DNA were detected in saliva from the healthy individuals in our sample. PCR methodology thus proved to be a useful tool for Roseolovirus detection and it is important to consider possible geographic and populations differences that could explain the comparatively low prevalence rates described here.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	1678-8060 0074-0276 1678-8060 0074-0276
DOI:	10.1590/S0074-02762010000700015