The Parameters of Transcutaneous Electrical Nerve Stimulation Are Critical to Its Regenerative Effects When Applied Just after a Sciatic Crush Lesion in Mice

We investigated the effect of two frequencies of transcutaneous electrical nerve stimulation (TENS) applied immediately after lesion on peripheral nerve regeneration after a mouse sciatic crush injury. The animals were anesthetized and subjected to crushing of the right sciatic nerve and then separa...

Full description

Saved in:

Bibliographic Details
Published in:	BioMed research international Vol. 2014; no. 2014; pp. 1 - 8
Main Authors:	Cavalcante Miranda de Assis, Diana, Martins Lima, Êmyle, Teixeira Goes, Bruno, Zugaib Cavalcanti, João, Barbosa Paixão, Alaí, Vannier-Santos, Marcos André, Martinez, Ana Maria Blanco, Baptista, Abrahão Fontes
Format:	Journal Article
Language:	English
Published:	Cairo, Egypt Hindawi Puplishing Corporation 01-01-2014 Hindawi Publishing Corporation John Wiley & Sons, Inc Hindawi Limited
Subjects:	Animals Biomedical research Brain-derived neurotrophic factor Colleges & universities Electrodes Gene expression Injuries Laboratories Medicine Mice Myelin Sheath - physiology Nerve Crush - methods Nerve Regeneration - physiology Nervous system Neural stimulation Neurons Patient outcomes Regeneration Rodents Sciatic nerve Sciatic Nerve - physiopathology Sciatic Neuropathy - physiopathology Transcutaneous Electric Nerve Stimulation - methods Transcutaneous electrical nerve stimulation-TENS
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	We investigated the effect of two frequencies of transcutaneous electrical nerve stimulation (TENS) applied immediately after lesion on peripheral nerve regeneration after a mouse sciatic crush injury. The animals were anesthetized and subjected to crushing of the right sciatic nerve and then separated into three groups: nontreated, Low-TENS (4 Hz), and High-TENS (100 Hz). The animals of Low- and High-TENS groups were stimulated for 2 h immediately after the surgical procedure, while the nontreated group was only positioned for the same period. After five weeks the animals were euthanized, and the nerves dissected bilaterally for histological and histomorphometric analysis. Histological assessment by light and electron microscopy showed that High-TENS and nontreated nerves had a similar profile, with extensive signs of degeneration. Conversely, Low-TENS led to increased regeneration, displaying histological aspects similar to control nerves. High-TENS also led to decreased density of fibers in the range of 6–12 μm diameter and decreased fiber diameter and myelin area in the range of 0–2 μm diameter. These findings suggest that High-TENS applied just after a peripheral nerve crush may be deleterious for regeneration, whereas Low-TENS may increase nerve regeneration capacity.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Academic Editor: Xiaofeng Jia
ISSN:	2314-6133 2314-6141
DOI:	10.1155/2014/572949