pSTAT3 Levels Have Divergent Expression Patterns and Associations with Survival in Squamous Cell Carcinoma and Adenocarcinoma of the Oesophagus

Signal transducers and activator of transcription (STAT)-3 is activated in cancers, where it promotes growth, inflammation, angiogenesis, and inhibits apoptosis. Tissue microarrays were generated using tissues from 154 patients, with oesophageal adenocarcinoma (OAC) ( = 116) or squamous cell carcino...

Full description

Saved in:

Bibliographic Details
Published in:	International journal of molecular sciences Vol. 19; no. 6; p. 1720
Main Authors:	O' Sullivan, Katie E, Michielsen, Adriana J, O' Regan, Esther, Cathcart, Mary C, Moore, Gillian, Breen, Eamon, Segurado, Ricardo, Reynolds, John V, Lysaght, Joanne, O' Sullivan, Jacintha
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 10-06-2018 MDPI
Subjects:	Adenocarcinoma Adenocarcinoma - metabolism Adenocarcinoma - mortality Adenocarcinoma - pathology Angiogenesis Apoptosis cancer Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - mortality Carcinoma, Squamous Cell - pathology Cell Movement Cell Proliferation Cell Survival Epithelium Esophageal cancer Esophageal Neoplasms - metabolism Esophageal Neoplasms - mortality Esophageal Neoplasms - pathology Esophagus Humans Immunohistochemistry Inhibitors Interleukin 6 oesophagus Patients Phosphorylation Proportional Hazards Models Receptors, Interleukin-6 - metabolism Squamous cell carcinoma STAT3 Stat3 protein STAT3 Transcription Factor - metabolism Survival Survival Analysis Tissue Array Analysis Transcription Transducers Tumors adenocarcinoma cancer oesophagus STAT3 squamous cell carcinoma
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Signal transducers and activator of transcription (STAT)-3 is activated in cancers, where it promotes growth, inflammation, angiogenesis, and inhibits apoptosis. Tissue microarrays were generated using tissues from 154 patients, with oesophageal adenocarcinoma (OAC) ( = 116) or squamous cell carcinoma (SCC) ( = 38) tumours. The tissues were stained for pSTAT3 and IL-6R using immunohistochemistry. The OE33 (OAC) and OE21 (SCC) cell lines were treated with the STAT3 inhibitor, STATTIC. The Univariate cox regression analysis revealed that a positive pSTAT3 in SCC was adversely associated with survival (Hazard ratio (HR) 6.382, 95% CI 1.266⁻32.184), while a protective effect was demonstrated with the higher pSTAT3 levels in OAC epithelium (HR 0.74, 95% CI 0.574⁻0.953). The IL-6R intensity levels were higher in the SCC tumours compared with the OAC tumours for the core and leading edge tumour tissue. The pSTAT3 levels correlated positively with the IL-6R levels in both the OAC and SCC. The treatment of OE21 and OE33 cells with the STAT3 inhibitor STATTIC in vitro resulted in decreased survival, proliferation, migration, and increased apoptosis. The pSTAT3 expression was associated with adverse survival in SCC, but not in the OAC patients. The inhibition of STAT3 in both of the tumour subtypes resulted in alterations in the survival, proliferation, migration, and apoptosis, suggesting a potential role for therapeutically targeting STAT3.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1422-0067 1661-6596 1422-0067
DOI:	10.3390/ijms19061720