Search Results - "Catalano, Carlos Enrique"

Strong subunit coordination drives a powerful viral DNA packaging motor by Andrews, Benjamin T., Catalano, Carlos Enrique

“…Terminase enzymes are viral motors that package DNA into a preformed capsid and are of interest both therapeutically and as potential nano-machines. The…”
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Major capsid reinforcement by a minor protein in herpesviruses and phage by Sae-Ueng, Udom, Liu, Ting, Catalano, Carlos Enrique, Huffman, Jamie B, Homa, Fred L, Evilevitch, Alex

“…Herpes simplex type 1 virus (HSV-1) and bacteriophage λ capsids undergo considerable structural changes during self-assembly and DNA packaging. The initial…”
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Thermodynamic Interrogation of the Assembly of a Viral Genome Packaging Motor Complex by Yang, Teng-Chieh, Ortiz, David, Nosaka, Lyn’Al, Lander, Gabriel C., Catalano, Carlos Enrique

“…Viral terminase enzymes serve as genome packaging motors in many complex double-stranded DNA viruses. The functional motors are multiprotein complexes that…”
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Binding then bending: A mechanism for wrapping DNA by Khrapunov, Sergei, Brenowitz, Michael, Rice, Phoebe A, Catalano, Carlos Enrique

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Cloning, Expression, and Biochemical Characterization of Hexahistidine-tagged Terminase Proteins by Hang, Qi, Woods, Liping, Feiss, Michael, Catalano, Carlos Enrique

“…The terminase enzyme from bacteriophage λ is composed of two viral proteins (gpA, 73.2 kDa; gpNu1, 20.4 kDa) and is responsible for packaging viral DNA into…”
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Biophysical Characterization of the DNA Binding Domain of gpNu1, a Viral DNA Packaging Protein by Bain, David L., Berton, Nancy, Ortega, Marcos, Baran, Jennifer, Yang, Qin, Catalano, Carlos Enrique

“…Terminase enzymes are common to double-stranded DNA viruses. These enzymes “package” the viral genome into a pre-formed capsid. Terminase from bacteriophage λ…”
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Thermodynamic Characterization of Viral Procapsid Expansion into a Functional Capsid Shell by Medina, Elizabeth, Nakatani, Eri, Kruse, Shannon, Catalano, Carlos Enrique

“…The assembly of “complex” DNA viruses such as the herpesviruses and many tailed bacteriophages includes a DNA packaging step where the viral genome is inserted…”
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The Enzymology of a Viral Genome Packaging Motor Is Influenced by the Assembly State of the Motor Subunits by Andrews, Benjamin T, Catalano, Carlos Enrique

“…Terminase enzymes are responsible for the excision of a single genome from a concatemeric precursor (genome maturation) and concomitant packaging of DNA into…”
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Molecular Dissection of the Forces Responsible for Viral Capsid Assembly and Stabilization by Decoration Proteins by Lambert, Shannon, Yang, Qin, De Angeles, Rolando, Chang, Jenny R, Ortega, Marcos, Davis, Christal, Catalano, Carlos Enrique

“…Complex double-stranded DNA viruses utilize a terminase enzyme to package their genomes into a preassembled procapsid shell. DNA packaging triggers a major…”
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Integration Host Factor Assembly at the Cohesive End Site of the Bacteriophage Lambda Genome: Implications for Viral DNA Packaging and Bacterial Gene Regulation by Sanyal, Saurarshi J., Yang, Teng-Chieh, Catalano, Carlos Enrique

“…Integration host factor (IHF) is an Escherichia coli protein involved in (i) condensation of the bacterial nucleoid and (ii) regulation of a variety of…”
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Analytical Ultracentrifugation as a Tool to Study Nonspecific Protein-DNA Interactions by Yang, Teng-Chieh, Catalano, Carlos Enrique, Maluf, Nasib Karl

“…Analytical ultracentrifugation (AUC) is a powerful tool that can provide thermodynamic information on associating systems. Here, we discuss how to use the two…”
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Challenging Packaging Limits and Infectivity of Phage λ by Nurmemmedov, Elmar, Castelnovo, Martin, Medina, Elizabeth, Catalano, Carlos Enrique, Evilevitch, Alex

“…The terminase motors of bacteriophages have been shown to be among the strongest active machines in the biomolecular world, being able to package several tens…”
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A Pseudo-Atomic Model for the Capsid Shell of Bacteriophage Lambda Using Chemical Cross-Linking/Mass Spectrometry and Molecular Modeling by Singh, Pragya, Nakatani, Eri, Goodlett, David R., Catalano, Carlos Enrique

“…Bacteriophage lambda is one of the most exhaustively studied of the double-stranded DNA viruses. Its assembly pathway is highly conserved among the…”
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Assembly and Maturation of the Bacteriophage Lambda Procapsid: gpC Is the Viral Protease by Medina, Elizabeth, Wieczorek, Doug, Medina, Eva Margarita, Yang, Qin, Feiss, Michael, Catalano, Carlos Enrique

“…Viral capsids are robust structures designed to protect the genome from environmental insults and deliver it to the host cell. The developmental pathway for…”
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The Bacteriophage Lambda gpNu3 Scaffolding Protein Is an Intrinsically Disordered and Biologically Functional Procapsid Assembly Catalyst by Medina, Eva Margarita, Andrews, Benjamin T., Nakatani, Eri, Catalano, Carlos Enrique

“…Procapsid assembly is a process whereby hundreds of copies of a major capsid protein assemble into an icosahedral protein shell into which the viral genome is…”
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Assembly and characterization of gp160-nanodiscs: A new platform for biochemical characterization of HIV envelope spikes by Nakatani-Webster, Eri, Hu, Shiu-Lok, Atkins, William M., Catalano, Carlos Enrique

“…•Lipid nanodiscs containing full-length glycoslyated gp160 Env proteins are prepared.•The particles are soluble and homogeneous compared to…”
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Biophysics of viral infectivity: matching genome length with capsid size by Nurmemmedov, Elmar, Castelnovo, Martin, Catalano, Carlos Enrique, Evilevitch, Alex

“…In this review, we discuss recent advances in biophysical virology, presenting experimental and theoretical studies on the physical properties of viruses. We…”
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Packaging of a Unit-Length Viral Genome: The Role of Nucleotides and the gpD Decoration Protein in Stable Nucleocapsid Assembly in Bacteriophage λ by Yang, Qin, Maluf, Nasib Karl, Catalano, Carlos Enrique

“…The developmental pathways for a variety of eukaryotic and prokaryotic double-stranded DNA viruses include packaging of viral DNA into a preformed procapsid…”
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Assembly of Bacteriophage Lambda Terminase into a Viral DNA Maturation and Packaging Machine by Maluf, Nasib Karl, Gaussier, Hélène, Bogner, Elke, Feiss, Michael, Catalano, Carlos Enrique

“…Terminase enzymes are common to complex double-stranded DNA viruses and function to package viral DNA into the capsid. We recently demonstrated that the…”
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Building a Virus from Scratch: Assembly of an Infectious Virus Using Purified Components in a Rigorously Defined Biochemical Assay System by Gaussier, Hélène, Yang, Qin, Catalano, Carlos Enrique

“…The assembly of double-stranded DNA (dsDNA) viruses such as poxvirus, the herpesviruses and many bacteriophages is a complex process that requires the…”
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