Production of transgenic goat (Capra hircus) with human Granulocyte Colony Stimulating Factor (hG-CSF) gene in Brazil

Production of transgenic goat (Capra hircus) with human Granulocyte Colony Stimulating Factor (hG-CSF) gene in Brazil

In order to produce transgenic goats with hG-CSF, a total of 24 adult Saanen and 48 adult undefined breed goats were used as donors and recipients, respectively. Donors were estrus-synchronized with vaginal sponges and superovulated by a treatment with 200 mg FSH given twice daily in decreasing dose...

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Bibliographic Details
Published in:Anais da Academia Brasileira de Ciências Vol. 79; no. 4; pp. 585 - 592
Main Authors: Freitas, Vicente J F, Serova, Irina A, Andreeva, Lyudmila E, Dvoryanchikov, Guennadi A, Lopes, Jr, Edilson S, Teixeira, Dárcio I A, Dias, Luciene P B, Avelar, Suely R G, Moura, Raylene R, Melo, Luciana M, Pereira, Alexsandra F, Cajazeiras, João B, Andrade, Maria L L, Almeida, Karlliely C, Castro, Karlliely A, Sousa, Francisco C, Carvalho, Antonio C C, Serov, Oleg L
Format: Journal Article
Language:English
Published: Brazil Academia Brasileira de Ciências 01-12-2007
Subjects:
Animals
Animals, Genetically Modified - embryology
Brazil
Capra hircus
caprino
DNA microinjection
embrião
embryo
Embryo Transfer
Female
goat
Goats - embryology
Goats - genetics
Granulocyte Colony-Stimulating Factor - genetics
hG-CSF
Male
Microinjections
microinjeção de DNA
Multidisciplinarias (Ciencias Sociales y Humanidades)
MULTIDISCIPLINARY SCIENCES
Pregnancy
transgenesis
transgênese
Zygote - physiology
Brazil
microinjeção de DNA
transgenesis
caprino
embryo
goat
hG-CSF
DNA microinjection
transgênese
embrião
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Summary:In order to produce transgenic goats with hG-CSF, a total of 24 adult Saanen and 48 adult undefined breed goats were used as donors and recipients, respectively. Donors were estrus-synchronized with vaginal sponges and superovulated by a treatment with 200 mg FSH given twice daily in decreasing doses over 3 days starting 48 h before sponge removal. Ovulation was induced by injecting 100 microg GnRH 36 h after sponge removal. The recipients also received an estrus synchronization treatment. Donors were mated with fertile Saanen bucks and, approximately 72 h after sponge removal, zygotes were recovered surgically by flushing oviducts. The recovered zygotes were briefly centrifuged to a reliable visualization of the pronuclei. The DNA construct containing hG-CSF gene flanked by goat and bovine alphas1-casein sequences was injected into pronuclei of 129 zygotes. The microinjected embryos (3-6 per female) were transferred to 27 recipients. Ten recipients became pregnant and 12 kids were born. One transgenic male founder was identified in the group of kids. This is the first report of a birth of a transgenic goat in Latin America.
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ISSN:0001-3765
1678-2690
0001-3765
1678-2690
DOI:10.1590/s0001-37652007000400003