High frequency of hemolytic and cytotoxic activity in Aeromonas spp. isolated from clinical, food and environmental in Rio de Janeiro, Brazil

Molecular study of aerolysin and cytotonic enterotoxin genes by PCR and colony blot hybridization was performed in 117 strains of Aeromonas spp. isolated from different sources. Homogeneous distribution of these genes in A. hydrophila complex strains was observed. For A. caviae and A. sobria complex...

Full description

Saved in:

Bibliographic Details
Published in:	Antonie van Leeuwenhoek Vol. 96; no. 1; pp. 53 - 61
Main Authors:	Castilho, Magda C. B, Castro, Tatiana L. A, Araújo, Viviane S, Trajano, Rosemere S, Santos, Paula A, Pimenta, Paula M. C, Lucheze, Kely, Melo, Janyne T. B, Gonçalves, Aline M, Nogueira, Raquel T, de Luna, Maria Graças, Freitas-Almeida, Angela C
Format:	Journal Article
Language:	English
Published:	Dordrecht Dordrecht : Springer Netherlands 01-06-2009 Springer Netherlands Springer Springer Nature B.V
Subjects:	Aeromonas Aeromonas - isolation & purification Aeromonas - pathogenicity Aeromonas veronii Bacteria Bacterial Toxins - genetics Bacteriology Biological and medical sciences Biomedical and Life Sciences Brazil Cell Death Environmental Microbiology Food Microbiology Freshwater Fundamental and applied biological sciences. Psychology Genes Genotype Gram-Negative Bacterial Infections - microbiology Hemolysis Humans Life Sciences Medical Microbiology Microbiology Miscellaneous Nucleic Acid Hybridization Original Paper Plant Sciences Polymerase Chain Reaction Soil Science & Conservation Studies Virulence Factors - genetics South America Brazil America Rio de Janeiro Brazil Brazil, Rio de Janeiro spp Aerolysin genes Virulence factors PCR Human Aeromonas Virulence Polymerase chain reaction Toxin Gene Enterotoxin Aeromonas spp Bacteria Vibrionaceae Environment Biological contamination Clinical isolate Food
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Molecular study of aerolysin and cytotonic enterotoxin genes by PCR and colony blot hybridization was performed in 117 strains of Aeromonas spp. isolated from different sources. Homogeneous distribution of these genes in A. hydrophila complex strains was observed. For A. caviae and A. sobria complex strains, aerolysin genes were more frequent than cytotonic enterotoxins genes. Of 64 A. caviae complex strains, only one (1.5%) amplified the 451 bp product for the aer gene, however, the same primers detected a 400 bp product in 50 (78%) strains. This product was sequenced and had two short regions with homology to several hemolysin genes. The genotype aer ⁺/aerA⁺/hly ⁺/ast ⁺/alt ⁺ was detected in six A. hydrophila strains from food and environmental source. The most common genotype found in A. hydrophila strains was hly ⁺ (85%) and aerA⁺ (78.7%), while in A. caviae complex strains was aerA⁺ (32.8%). All A. veronii complex sobria strains were aer ⁺/aerA⁺. All A. caviae and A. hydrophila were positive when tested with aer probe using the colony blot test. Thirty-seven percent of A. hydrophila and 53% of A. caviae tested were positive for ast probe. Eighty-nine percent of samples were cytotoxic in Vero cells. Our data demonstrated that Aeromonas spp. can harbor and express virulence genes and reinforce the potential of Aeromonas as a human pathogen.
Bibliography:	http://dx.doi.org/10.1007/s10482-009-9335-6 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0003-6072 1572-9699
DOI:	10.1007/s10482-009-9335-6