Chromosome banding and DNA methylation patterns, chromatin organisation and nuclear DNA content in Zingeria biebersteiniana

Chromosome structure and chromatin organisation of a two-chromosome model cereal Zingeria biebersteiniana were studied: nuclear DNA content was determined by microdensitometric analysis after Feulgen staining; Feulgen absorption at different thresholds of absorbance in interphase nuclei also provide...

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Bibliographic Details
Published in:	Biologia plantarum Vol. 46; no. 4; pp. 543 - 550
Main Authors:	Cremonini, R, Castiglione, M.R. (University of Pisa (Italy). Dept. of Botanical Sciences), Grif, V.G, Kotseruba, V.V, Punina, E.O, Rodionov, A.V, Muravenko, O.V, Popov, K.V, Samatadze, T.E
Format:	Journal Article
Language:	English
Published:	Dordrecht Springer Nature B.V 01-01-2003
Subjects:	ABSORBANCE ABSORBANCIA ADN ANALYSE D'IMAGE ANÁLISIS DE IMÁGENES BANDEO DE CROMOSOMAS BANDING CHROMOSOMIQUE CEREALES CEREALS CHROMATIN CHROMATINE CHROMOSOME CHROMOSOME BANDING CHROMOSOMES CROMATINA CROMOSOMAS Cytosine CÉRÉALE Deoxyribonucleic acid DNA DNA methylation Fluorophores Heterochromatin Homology IMAGE ANALYSIS Image processing Image segmentation LARGURA LENGTH LONGUEUR Metaphase METHYLATION METILACIÓN Monoclonal antibodies MÉTHYLATION Nuclei POACEAE Staining ULTAESTRUCTURA ULTRASTRUCTURE Zingeria biebersteiniana
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Summary:	Chromosome structure and chromatin organisation of a two-chromosome model cereal Zingeria biebersteiniana were studied: nuclear DNA content was determined by microdensitometric analysis after Feulgen staining; Feulgen absorption at different thresholds of absorbance in interphase nuclei also provided evidence on the organisation of chromatin, allowing quantitative estimation of condensed chromatin within interphasic nucleus. The DNA methylation pattern of Z. biebersteiniana metaphase chromosomes was examined with a specific monoclonal antibody. 5-methyl-cytosine residues are present in several chromosome sites and differences may be present between corresponding regions of homologues. Chromosome banding pattern reveals large bands in the centromeric regions of each chromosome, showing constitutive heterochromatin; by fluorochromes staining pericentromeric blocks are evidenced. After the cold and 9-aminoacridine pre-treatments and after aceto-carmine and aceto-orceine staining, respectively, the metaphase chromosomes were analysed by image analysis system revealing a segmentation of the chromosome body that resembles Giemsa/Reverse banding in animal chromosomes.
Bibliography:	F50 F30 2004000085 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0006-3134 1573-8264
DOI:	10.1023/A:1024863511570