PHA production, from bacteria to plants

The genes encoding the polyhydroxyalkanoate (PHA) biosynthetic pathway in Ralstonia eutropha (3-ketothiolase, phaA or bktB; acetoacetyl-CoA reductase, phaB; and PHA synthase, phaC) were engineered for plant plastid targeting and expressed using leaf (e35S) or seed-specific (7s or lesquerella hydroxy...

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Bibliographic Details
Published in:	International journal of biological macromolecules Vol. 25; no. 1; pp. 303 - 306
Main Authors:	Valentin, Henry E., Broyles, Debra L., Casagrande, Laura A., Colburn, Susan M., Creely, Wendi L., DeLaquil, Pamela A., Felton, Heather M., Gonzalez, Kathleen A., Houmiel, Kathryn L., Lutke, Kevin, Mahadeo, Debbie A., Mitsky, Timothy A., Padgette, Stephen R., Reiser, Steven E., Slater, Steven, Stark, David M., Stock, Robert T., Stone, Deborah A., Taylor, Nancy B., Thorne, Gregory M., Tran, Minhtien, Gruys, Kenneth J.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-06-1999
Subjects:	Acetyl-CoA C-Acyltransferase - genetics Acetyl-CoA C-Acyltransferase - metabolism Acyltransferases - genetics Acyltransferases - metabolism Alcohol Oxidoreductases - genetics Alcohol Oxidoreductases - metabolism Arabidopsis - genetics Arabidopsis - metabolism Cupriavidus necator - enzymology Cupriavidus necator - genetics Homozygote Models, Molecular Molecular Structure Plant Leaves Plants - metabolism Plants, Genetically Modified Polyesters - metabolism Recombinant Proteins - metabolism Seeds
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Summary:	The genes encoding the polyhydroxyalkanoate (PHA) biosynthetic pathway in Ralstonia eutropha (3-ketothiolase, phaA or bktB; acetoacetyl-CoA reductase, phaB; and PHA synthase, phaC) were engineered for plant plastid targeting and expressed using leaf (e35S) or seed-specific (7s or lesquerella hydroxylase) promoters in Arabidopsis and Brassica. PHA yields in homozygous transformants were 12–13% of the dry mass in homozygous Arabidopsis plants and approximately 7% of the seed weight in seeds from heterozygous canola plants. When a threonine deaminase was expressed in addition to bktB, phaB and phaC, a copolyester of 3-hydroxybutyrate and 3-hydroxyvalerate was produced in both Arabidopsis and Brassica.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0141-8130 1879-0003
DOI:	10.1016/S0141-8130(99)00045-8