Search Results - "Carvalho, Sofia B"

Downstream processing for influenza vaccines and candidates: An update by B. Carvalho, Sofia, Peixoto, Cristina, T. Carrondo, Manuel J., S. Silva, Ricardo J.

“…Seasonal and pandemic influenza outbreaks present severe health and economic burdens. To overcome limitations on influenza vaccines' availability and…”
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Multi attribute method implementation using a High Resolution Mass Spectrometry platform: From sample preparation to batch analysis by Carvalho, Sofia B, Gomes, Ricardo A, Pfenninger, Anja, Fischer, Martina, Strotbek, Michaela, Isidro, Inês A, Tugçu, Nihal, Gomes-Alves, Patrícia

“…Quality control of biopharmaceuticals such as monoclonal antibodies (mAbs) has been evolving and becoming more challenging as the requirements of the…”
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Purification of influenza virus‐like particles using sulfated cellulose membrane adsorbers by B Carvalho, Sofia, Fortuna, A Raquel, Wolff, Michael W, Peixoto, Cristina, M Alves, Paula, Reichl, Udo, JT Carrondo, Manuel

“…ABSTRACT BACKGROUND Vaccines based on virus‐like particles (VLPs) are an alternative to inactivated viral vaccines that combine good safety profiles with…”
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A detection and quantification label-free tool to speed up downstream processing of model mucins by Carvalho, Sofia B, Moreira, Ana Sofia, Gomes, Joana, Carrondo, Manuel J T, Thornton, David J, Alves, Paula M, Costa, Julia, Peixoto, Cristina

“…Mucins are high-molecular weight glycoproteins (0.25-20 MDa) containing one or more domains that are heavily O-glycosylated. Their implications as targets for…”
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Assessing Multi-Attribute Characterization of Enveloped and Non-Enveloped Viral Particles by Capillary Electrophoresis by Fernandes, Rita P, Escandell, José M, Guerreiro, Ana C L, Moura, Filipa, Faria, Tiago Q, Carvalho, Sofia B, Silva, Ricardo J S, Gomes-Alves, Patrícia, Peixoto, Cristina

“…Virus-based biopharmaceutical products are used in clinical applications such as vaccines, gene therapy, and immunotherapy. However, their manufacturing…”
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Universal label‐free in‐process quantification of influenza virus‐like particles by Carvalho, Sofia B., Moleirinho, Mafalda G., Wheatley, David, Welsh, John, Gantier, René, Alves, Paula M., Peixoto, Cristina, Carrondo, Manuel J. T.

“…Virus‐like particles (VLPs) are becoming established as vaccines, in particular for influenza pandemics, increasing the interest in the development of VLPs…”
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Extracellular Vesicles from Ovarian Carcinoma Cells Display Specific Glycosignatures by Gomes, Joana, Gomes-Alves, Patrícia, Carvalho, Sofia B, Peixoto, Cristina, Alves, Paula M, Altevogt, Peter, Costa, Julia

“…Cells release vesicles to the extracellular environment with characteristic nucleic acid, protein, lipid, and glycan composition. Here we have isolated and…”
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Biolayer Interferometry Analysis for a Higher Throughput Quantification of In-Process Samples of a Rotavirus Vaccine by Carvalho, Sofia B, Dias, Mafalda M, Matheise, Jean-Philippe, Knott, Isabelle, Gomes-Alves, Patrícia, Alves, Paula M

“…Rotavirus A infection is a global leading cause of severe acute gastroenteritis associated with life-threatening diarrheal episodes in infants and young…”
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Intrinsically disordered and aggregation prone regions underlie β-aggregation in S100 proteins by Carvalho, Sofia B, Botelho, Hugo M, Leal, Sónia S, Cardoso, Isabel, Fritz, Günter, Gomes, Cláudio M

“…S100 proteins are small dimeric calcium-binding proteins which control cell cycle, growth and differentiation via interactions with different target proteins…”
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Bioanalytics for Influenza Virus-Like Particle Characterization and Process Monitoring by Carvalho, Sofia B, Silva, Ricardo J S, Sousa, Marcos F Q, Peixoto, Cristina, Roldão, António, Carrondo, Manuel J T, Alves, Paula M

“…Virus-like particles (VLPs) are excellent platforms for the development of influenza vaccine candidates. Nonetheless, their characterization is challenging due…”
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Leveraging rAAV bioprocess understanding and next generation bioanalytics development by Escandell, Jose M, Pais, Daniel AM, Carvalho, Sofia B, Vincent, Karen, Gomes-Alves, Patrícia, Alves, Paula M

“…[Display omitted] •Highlight CMC strategies to address the increasing rAAV vector demand.•Outline solutions in the areas of cell line development and upstream…”
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SWATH-MS as a strategy for CHO host cell protein identification and quantification supporting the characterization of mAb purification platforms by Carvalho, Sofia B., Profit, Ludivine, Krishnan, Sushmitha, Gomes, Ricardo A., Alexandre, Bruno M., Clavier, Severine, Hoffman, Michael, Brower, Kevin, Gomes-Alves, Patrícia

“…Host cell proteins (HCPs) are process-related impurities expressed by the host cells during biotherapeutics’ manufacturing, such as monoclonal antibodies…”
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Towards a scalable bioprocess for rAAV production using a HeLa stable cell line by Escandell, José, Moura, Filipa, Carvalho, Sofia B., Silva, Ricardo J. S., Correia, Ricardo, Roldão, Antonio, Gomes‐Alves, Patrícia, Alves, Paula M.

“…The majority of recombinant adeno‐associated viruses (rAAV) approved for clinical use or in clinical trials areproduced by transient transfection using the…”
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Bioprocess integration for human mesenchymal stem cells: From up to downstream processing scale-up to cell proteome characterization by Cunha, Bárbara, Aguiar, Tiago, Carvalho, Sofia B., Silva, Marta M., Gomes, Ricardo A., Carrondo, Manuel J.T., Gomes-Alves, Patrícia, Peixoto, Cristina, Serra, Margarida, Alves, Paula M.

“…•Prove scalability of an integrated, robust and closed bioprocess for BM-MSC and AT-MSC using single-use technology.•hMSC expansion scale-up ensured high…”
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Efficient filtration strategies for the clarification of influenza virus-like particles derived from insect cells by Carvalho, Sofia B., Silva, Ricardo J.S., Moreira, Ana Sofia, Cunha, Bárbara, Clemente, João J., Alves, Paula M., Carrondo, Manuel J.T., Xenopoulos, Alex, Peixoto, Cristina

“…•The best filter types for the clarification stage of influenza Virus-like particles were identified.•Primary and secondary clarification filters were…”
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Membrane‐Based Approach for the Downstream Processing of Influenza Virus‐Like Particles by Carvalho, Sofia B., Silva, Ricardo J. S., Moleirinho, Mafalda G., Cunha, Bárbara, Moreira, Ana S., Xenopoulos, Alex, Alves, Paula M., Carrondo, Manuel J. T., Peixoto, Cristina

“…Currently, marketed influenza vaccines are only efficient against homologous viruses, thus requiring a seasonal update based on circulating subtypes. This…”
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Bioorthogonal Strategy for Bioprocessing of Specific-Site-Functionalized Enveloped Influenza-Virus-Like Particles by Carvalho, Sofia B, Freire, João M, Moleirinho, Mafalda G, Monteiro, Francisca, Gaspar, Diana, Castanho, Miguel A. R. B, Carrondo, Manuel J. T, Alves, Paula M, Bernardes, Gonçalo J. L, Peixoto, Cristina

“…Virus-like particles (VLPs) constitute a promising platform in vaccine development and targeted drug delivery. To date, most applications use simple…”
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Baculovirus affinity removal in viral-based bioprocesses by Moleirinho, Mafalda G., Fernandes, Rita P., Carvalho, Sofia B., Bezemer, Sandra, Detmers, Frank, Hermans, Pim, Silva, Ricardo J.S., Alves, Paula M., Carrondo, Manuel J.T., Peixoto, Cristina

“…Schematic representation of a strategy for impurity removal of an influenza vaccine candidate. [Display omitted] •Discovery of affinity ligands for baculovirus…”
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Using TEM-Based Image Analysis to Validate the Presence of HA Spikes on Influenza VLPs by Ryner, Martin, Forsberg, Nina, Carvalho, Vanessa, Peixoto, Cristina, Carvalho, Sofia B

“…In this study, a complete membrane-based downstream process for the purification of influenza virus-like particles (VLPs) was monitored through transmission…”
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