Biosensor Characterization from Cratylia mollis Seed Lectin (Cramoll)‐MOF and Specific Carbohydrate Interactions in an Electrochemical Model

Biosensor Characterization from Cratylia mollis Seed Lectin (Cramoll)‐MOF and Specific Carbohydrate Interactions in an Electrochemical Model

Biosensors are small devices known for their selectivity, high specificity and sensitivity to the respective analyte, at low concentrations. We developed an electrochemical biosensor using the crystalline polymer MOF‐[Cu3(BTC)2(H2O)2]n to characterize Cratylia mollis seed lectin (Cramoll) and its in...

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Bibliographic Details
Published in:Chemistry & biodiversity Vol. 19; no. 12; pp. e202200515 - n/a
Main Authors: Carvalho, Maryana Hermínio, Araújo, Hallysson Douglas Andrade, Silva, Renata Pereira, Santos Correia, Maria Tereza, Freitas, Katia Cristina Silva, Souza, Sandra Rodrigues, Barroso Coelho, Luana Cassandra Breitenbach
Format: Journal Article
Language:English
Published: Switzerland Wiley Subscription Services, Inc 01-12-2022
Subjects:
Animals
Biological models (mathematics)
Biosensing Techniques
Biosensors
Carbohydrates
Carbohydrates - analysis
Carbohydrates - chemistry
Cratylia mollis
cyclic voltammograms
Electrical measurement
Electrochemistry
electrode surface
Electrodes
Erythrocytes
Fabaceae - chemistry
Glucose
immobilization
Interface stability
Lectins
Lectins - chemistry
Low concentrations
metal-organic framework (MOF)
Metal-organic frameworks
plant lectin
Polymers
Rabbits
Scanning electron microscopy
Seeds - chemistry
Selectivity
Sensitivity analysis
Surface stability
cyclic voltammograms
metal-organic framework (MOF)
immobilization
plant lectin
electrode surface
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Summary:Biosensors are small devices known for their selectivity, high specificity and sensitivity to the respective analyte, at low concentrations. We developed an electrochemical biosensor using the crystalline polymer MOF‐[Cu3(BTC)2(H2O)2]n to characterize Cratylia mollis seed lectin (Cramoll) and its interaction with free carbohydrate (glucose) and carbohydrates on the surface of rabbit erythrocytes. The electrochemical potentials presented by the exponential curves that vary from 96 to 142 mV in relation to concentrations of 10 to 20 mM of glucose are decisive for the use of the system containing gold electrode/MOF/Cramoll for the characterization of biological models due to its high sensitivity. As well as the kinetic behavior presented in the cyclic voltammograms, with a cathodic current response of 0.000 3 A for a glucose concentration of 15 mM. These results were due to the high specificity of Cramoll under these conditions, promoting stability of surface charges at the Cramoll/electrode interface. This phenomenon facilitates the monitoring of the interaction with free glucose present in the electrolyte medium by potentiometric and amperometric methods and with carbohydrates present on the surface of rabbit erythrocytes through the potentiometric method. Through scanning electron microscopy (SEM) it was possible to observe Cramoll immobilized on the MOF surface, proving the specificity of the ligand (glucose‐lectin) through the morphological lectin changes in this process. This electrochemical model, Cramoll/MOF biosensor, is effective for evaluating free lectin/carbohydrate or in the erythrocyte membrane.
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ISSN:1612-1872
1612-1880
DOI:10.1002/cbdv.202200515