Identification and genetic diversity of Fusarium sacchari associated with pokkah boeng disease of sugarcane in Northeast Brazil

Identification and genetic diversity of Fusarium sacchari associated with pokkah boeng disease of sugarcane in Northeast Brazil

Isolates from the Fusarium fujikuroi species complex, mainly F. sacchari, have been reported to be the causal agents of pokkah boeng in sugarcane in Brazil. However, inadequate information was available on the occurrence and genetic diversity of F. sacchari in Northeast Brazil, which is a limiting f...

Full description

Saved in:
Bibliographic Details
Published in:Journal of phytopathology Vol. 171; no. 1; pp. 36 - 47
Main Authors: Carvalho, Maria Raquel Veras, Rocha, Kelvim Crist Araujo, Melo, Maruzanete Pereira, Britto, Fábio Barros, Barguil, Beatriz Meireles, Alcântara Neto, Francisco, Borges, Abel Ferreira, Silva, Juliana Pereira, Beserra, José Evando Aguiar
Format: Journal Article
Language:English
Published: Berlin Wiley Subscription Services, Inc 01-01-2023
Subjects:
Ascospores
Cirri
Cultivars
Fertility
Fungicides
Fusarium fujikuroi
Fusarium fujikuroi species complex
Fusarium subglutinans
Gene flow
Gene polymorphism
Genetic diversity
Geographical locations
Hermaphrodites
ISSR
mating types
Perithecia
Polymorphism
Saccharum spp. hybrids
Signs and symptoms
Sugarcane
Brazil
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Isolates from the Fusarium fujikuroi species complex, mainly F. sacchari, have been reported to be the causal agents of pokkah boeng in sugarcane in Brazil. However, inadequate information was available on the occurrence and genetic diversity of F. sacchari in Northeast Brazil, which is a limiting factor on management. Thus, isolates of F. subglutinans sensu lato from sugarcane plants with symptoms of pokkah boeng were evaluated using the sexual cross‐fertility to determine species. All the isolates produced black perithecia when they were crossed with the test isolates of F. sacchari. Three weeks after the crossing, the formation of fertile ascospores cirri was observed. Thirty‐four isolates were self‐sterile hermaphrodites, while 21 were fertile only as males. Five isolates were homothallic. The effective size [Ne(f)] of the population as a function of the frequency of hermaphrodites and female sterile strains was 95.5%. The F. sacchari isolates were separated into four genetic groups independent of geographic location. The mean of polymorphism among all populations was 79%, and the average unbiased genetic diversity (uh) was considered moderate (0.31). This study in addition to confirming that F. sacchari as the main species associated with pokkah boeng in sugarcane in Northeast Brazil, reveals the relationship of mating type and genetic diversity of F. sacchari. The unrestricted gene flow between regions is probably the best explanation for the low geographic correlation. This knowledge will help in the adoption of management measures with fungicides or resistant cultivars.
Bibliography:Maria Raquel Veras de Carvalho and Kelvim Crist Araujo Rocha contributed equally to this work.
ISSN:0931-1785
1439-0434
DOI:10.1111/jph.13152