Elevated zinc induces siderophore biosynthesis genes and a zntA-like gene in Pseudomonas fluorescens

Zinc-regulated genes were analyzed in Pseudomonas fluorescens employing mutagenesis with a reporter gene transposon. Six mutants responded with increased gene expression to elevated concentrations of zinc. Genetic and biochemical analysis revealed that in four of the six mutants the transposon had i...

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Bibliographic Details
Published in:	FEMS microbiology letters Vol. 191; no. 1; pp. 61 - 70
Main Authors:	Rossbach, S, Wilson, T.L, Kukuk, M.L, Carty, H.A
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Publishing Ltd 01-10-2000 Blackwell Oxford University Press
Subjects:	Adenosine Triphosphatases - genetics Adenosine Triphosphatases - metabolism adenosinetriphosphatase Amino Acid Sequence Bacteriology beta-Galactosidase - genetics beta-Galactosidase - metabolism Biochemical analysis Biological and medical sciences Biosynthesis Cadmium copper DNA Transposable Elements essential genes Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Bacterial Genes Genetic analysis Genetics Ion transport Ions Metal stress Metals, Heavy - pharmacology Microbial Sensitivity Tests Microbiology Molecular Sequence Data Mutagenesis Mutants Mutation Pseudomonas fluorescens Pseudomonas fluorescens - drug effects Pseudomonas fluorescens - genetics Pseudomonas fluorescens - growth & development Pseudomonas fluorescens - metabolism Pyoverdine Pyoverdines P‐type ATPase Reporter gene reporter genes Siderophore siderophores Siderophores - biosynthesis Siderophores - genetics Similarity Transposons Zinc Zinc - metabolism Zinc - pharmacology Siderophore Pyoverdine P-type ATPase Zinc Metal stress Pseudomonadales Cadmium Enzyme Adenosinetriphosphatase Biosynthesis Pseudomonas fluorescens Metal Gene expression Heavy metal Regulation(control) Gene Bacteria Hydrolases Pseudomonadaceae
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Summary:	Zinc-regulated genes were analyzed in Pseudomonas fluorescens employing mutagenesis with a reporter gene transposon. Six mutants responded with increased gene expression to elevated concentrations of zinc. Genetic and biochemical analysis revealed that in four of the six mutants the transposon had inserted into genes essential for the biosynthesis of the siderophore pyoverdine. The growth of one of the mutants was severely impaired in the presence of elevated concentrations of cadmium and zinc ions. In this mutant, the transposon had inserted in a gene with high similarity to P-type ATPases involved in zinc and cadmium ion transport. Four mutants reacted with reduced gene expression to elevated concentrations of zinc. One of these mutants was sensitive to zinc, cadmium and copper ions. The genetic region targeted in this mutant did not show similarity to any known gene.
ISSN:	0378-1097 1574-6968
DOI:	10.1111/j.1574-6968.2000.tb09320.x