Search Results - "Carrondo, Manuel J.T"

Large-scale production and purification of VLP-based vaccines by Vicente, Tiago, Roldão, António, Peixoto, Cristina, Carrondo, Manuel J.T., Alves, Paula M.

“…Relevant strategies implemented to overcome the challenges of large-scale production (USP) and purification (DSP) of VLP-vaccines using the BEVS/IC. These…”
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Adaptive laboratory evolution of stable insect cell lines for improved HIV-Gag VLPs production by Fernandes, Bárbara, Vidigal, João, Correia, Ricardo, Carrondo, Manuel J.T., Alves, Paula M., Teixeira, Ana P., Roldão, António

“…•Adaptive laboratory evolution (ALE) of stable insect cell lines to hypothermic culture conditions.•Adapted cells express up to 26-fold more Gag-VLPs than…”
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Improving expansion of pluripotent human embryonic stem cells in perfused bioreactors through oxygen control by Serra, Margarida, Brito, Catarina, Sousa, Marcos F.Q., Jensen, Janne, Tostões, Rui, Clemente, João, Strehl, Raimund, Hyllner, Johan, Carrondo, Manuel J.T., Alves, Paula M.

“…The successful transfer of human embryonic stem cell (hESC) technology and cellular products into clinical and industrial applications needs to address issues…”
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Cell density effect in the baculovirus-insect cells system: A quantitative analysis of energetic metabolism by Bernal, Vicente, Carinhas, Nuno, Yokomizo, Adriana Y, Carrondo, Manuel J.T, Alves, Paula M

“…The cell density effect (i.e., the drop in the specific productivity in the baculovirus-insect cells expression system when cells are infected at high cell…”
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Cellular targets for improved manufacturing of virus-based biopharmaceuticals in animal cells by Rodrigues, Ana F, Carrondo, Manuel J.T, Alves, Paula M, Coroadinha, Ana S

“…Highlights • Vaccines and viral vectors produced in animal cells constitute a growing research field and market. • Virus–host interactions can unveil targets…”
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Perfusion of 3D encapsulated hepatocytes—A synergistic effect enhancing long‐term functionality in bioreactors by Tostões, Rui M, Leite, Sofia B, Miranda, Joana P, Sousa, Marcos, Wang, Daniel I.C, Carrondo, Manuel J.T, Alves, Paula M

“…Long‐term primary cultures of hepatocytes are essential for bioartificial liver (BAL) devices and to reduce and replace animal tests in lead candidate…”
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Rational design and optimization of downstream processes of virus particles for biopharmaceutical applications: Current advances by Vicente, Tiago, Mota, José P.B., Peixoto, Cristina, Alves, Paula M., Carrondo, Manuel J.T.

“…The advent of advanced therapies in the pharmaceutical industry has moved the spotlight into virus-like particles and viral vectors produced in cell culture…”
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Extending hepatocyte functionality for drug-testing applications using high-viscosity alginate-encapsulated three-dimensional cultures in bioreactors by Miranda, Joana P, Rodrigues, Armanda, Tostões, Rui M, Leite, Sofia, Zimmerman, Heiko, Carrondo, Manuel J T, Alves, Paula M

“…The maintenance of differentiated hepatocyte phenotype in vitro depends on several factors-in particular, on extracellular matrix interactions, for example,…”
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Exosome-based therapeutics: Purification using semi-continuous multi-column chromatography by Moleirinho, Mafalda G., Silva, Ricardo J.S., Carrondo, Manuel J.T., Alves, Paula M., Peixoto, Cristina

“…Exosomes are small nanovesicles released by the cells with a potential therapeutic application. A semi-continuous size exclusion chromatography process was…”
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Integrating high cell density cultures with adapted laboratory evolution for improved Gag‐HA virus‐like particles production in stable insect cell lines by Fernandes, Bárbara, Correia, Ricardo, Sousa, Marcos, Carrondo, Manuel J. T., Alves, Paula M., Roldão, António

“…Stable insect cell lines are emerging as an alternative to the insect cell‐baculovirus expression vector system (IC‐BEVS) for protein expression, benefiting…”
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Bioprocess integration for human mesenchymal stem cells: From up to downstream processing scale-up to cell proteome characterization by Cunha, Bárbara, Aguiar, Tiago, Carvalho, Sofia B., Silva, Marta M., Gomes, Ricardo A., Carrondo, Manuel J.T., Gomes-Alves, Patrícia, Peixoto, Cristina, Serra, Margarida, Alves, Paula M.

“…•Prove scalability of an integrated, robust and closed bioprocess for BM-MSC and AT-MSC using single-use technology.•hMSC expansion scale-up ensured high…”
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Improved virus purification processes for vaccines and gene therapy by Nestola, Piergiuseppe, Peixoto, Cristina, Silva, Ricardo R. J. S, Alves, Paula M., Mota, José P. B., Carrondo, Manuel J. T.

“…ABSTRACT The downstream processing of virus particles for vaccination or gene therapy is becoming a critical bottleneck as upstream titers keep improving…”
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Enabling PAT in insect cell bioprocesses: In situ monitoring of recombinant adeno‐associated virus production by fluorescence spectroscopy by Pais, Daniel A. M., Portela, Rui M. C., Carrondo, Manuel J. T., Isidro, Inês A., Alves, Paula M.

“…The process analytical technology (PAT) initiative shifted the bioprocess development mindset towards real‐time monitoring and control tools to measure…”
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Metabolic responses of CHO cells to limitation of key amino acids by Duarte, Tiago M., Carinhas, Nuno, Barreiro, Laura C., Carrondo, Manuel J. T., Alves, Paula M., Teixeira, Ana P.

“…ABSTRACT Chinese hamster ovary (CHO) cells are the predominant host for production of therapeutic glycoproteins. In particular, the glutamine‐synthetase (GS)…”
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Combining stable insect cell lines with baculovirus-mediated expression for multi-HA influenza VLP production by Sequeira, Daniela P, Correia, Ricardo, Carrondo, Manuel J.T, Roldão, António, Teixeira, Ana P, Alves, Paula M

“…Highlights • Combining stable and baculovirus-mediated expression for multi-HA influenza VLPs production. • Modular strategy as competitive as co-infection…”
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Efficient filtration strategies for the clarification of influenza virus-like particles derived from insect cells by Carvalho, Sofia B., Silva, Ricardo J.S., Moreira, Ana Sofia, Cunha, Bárbara, Clemente, João J., Alves, Paula M., Carrondo, Manuel J.T., Xenopoulos, Alex, Peixoto, Cristina

“…•The best filter types for the clarification stage of influenza Virus-like particles were identified.•Primary and secondary clarification filters were…”
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Metabolic signatures of GS-CHO cell clones associated with butyrate treatment and culture phase transition by Carinhas, Nuno, Duarte, Tiago M., Barreiro, Laura C., Carrondo, Manuel J. T., Alves, Paula M., Teixeira, Ana P.

“…ABSTRACT Chinese hamster ovary (CHO) cells are preferred hosts for the production of recombinant biopharmaceuticals. Efforts to optimize these bioprocesses…”
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Filtration methodologies for the clarification and concentration of human mesenchymal stem cells by Cunha, Bárbara, Peixoto, Cristina, Silva, Marta M., Carrondo, Manuel J.T., Serra, Margarida, Alves, Paula M.

“…Currently human mesenchymal stem cells (hMSC) are expanded using microcarrier-based stirred culture systems from one to hundreds of liters of culture volume to…”
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Production of oncolytic adenovirus and human mesenchymal stem cells in a single-use, Vertical-Wheel bioreactor system: Impact of bioreactor design on performance of microcarrier-based cell culture processes by Sousa, Marcos F. Q., Silva, Marta M., Giroux, Daniel, Hashimura, Yas, Wesselschmidt, Robin, Lee, Brian, Roldão, António, Carrondo, Manuel J. T., Alves, Paula M., Serra, Margarida

“…Anchorage‐dependent cell cultures are used for the production of viruses, viral vectors, and vaccines, as well as for various cell therapies and tissue…”
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Purification of influenza virus‐like particles using sulfated cellulose membrane adsorbers by B Carvalho, Sofia, Fortuna, A Raquel, Wolff, Michael W, Peixoto, Cristina, M Alves, Paula, Reichl, Udo, JT Carrondo, Manuel

“…ABSTRACT BACKGROUND Vaccines based on virus‐like particles (VLPs) are an alternative to inactivated viral vaccines that combine good safety profiles with…”
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