Sperm selection with density gradient centrifugation and swim up: effect on DNA fragmentation in viable spermatozoa

Subjects increasing sperm DNA fragmentation (sDF) during Density Gradient Centrifugation (DGC), a common sperm selection procedure in Assisted Reproduction Techniques (ARTs), experience a 50% lower probability of pregnancy. Hence, identification of these subjects is of clinical importance. Here, we...

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Bibliographic Details
Published in:	Scientific reports Vol. 9; no. 1; p. 7492
Main Authors:	Muratori, M., Tarozzi, N., Carpentiero, F., Danti, S., Perrone, F. M., Cambi, M., Casini, A., Azzari, C., Boni, L., Maggi, M., Borini, A., Baldi, E.
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 16-05-2019 Nature Publishing Group
Subjects:	13/31 38/71 692/308 692/4017 Adult Cell Separation - methods Centrifugation Centrifugation - methods Deoxyribonucleic acid DNA DNA Fragmentation Humanities and Social Sciences Humans Middle Aged multidisciplinary Multivariate analysis Pregnancy Science Science (multidisciplinary) Semen Analysis - methods Semen Analysis - standards Sensitivity and Specificity Sperm
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Summary:	Subjects increasing sperm DNA fragmentation (sDF) during Density Gradient Centrifugation (DGC), a common sperm selection procedure in Assisted Reproduction Techniques (ARTs), experience a 50% lower probability of pregnancy. Hence, identification of these subjects is of clinical importance. Here, we investigated whether such subjects are identified with higher accuracy detecting DNA fragmentation in viable (viable sDF) instead of total spermatozoa (total sDF) and whether swim up, an alternative procedure to DGC, does not increase sDF. With DGC, we identified 10/20 subjects increasing total sDF, and 2 more subjects using viable sDF. With swim up, we identified 8/40 subjects increasing total sDF, and 8 more subjects using viable sDF. In addition, viable sDF reveals more accurately the increase of the damage when it occurs. Finally, a multivariate analysis demonstrated that the proportional increase of sDF was higher after DGC respect to swim up. In conclusion, viable sDF is a more accurate parameter to reveal the increase of the damage by selection both with swim up and DGC. Swim up increases sDF in some samples, although at a lesser extent than DGC, suggesting that it should be used to select spermatozoa for ARTs when possible.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-019-43981-2