Adhesion and Growth of Cultured Human Gingival Fibroblasts on Periodontally Involved Root Surfaces Treated by Er:YAG Laser

Adhesion and Growth of Cultured Human Gingival Fibroblasts on Periodontally Involved Root Surfaces Treated by Er:YAG Laser

Background: The application of Er:YAG laser irradiation, approved in 1997 to be used on dental hard tissues, has been investigated for periodontal therapy. The aim of this study was to analyze the biocompatibility of root surfaces treated by Er:YAG laser. Methods: Adhesion and growth of cultured hum...

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Bibliographic Details
Published in:Journal of periodontology (1970) Vol. 74; no. 9; pp. 1368 - 1375
Main Authors: Feist, Iliria S., De Micheli, Giorgio, Carneiro, Silvia R.S., Eduardo, Carlos P., Miyagi, Sueli P.H., Marques, Márcia M.
Format: Journal Article
Language:English
Published: 737 N. Michigan Avenue, Suite 800, Chicago, IL 60611‐2690, USA American Academy of Periodontology 01-09-2003
Subjects:
Adult
Aged
Aluminum Silicates
Cell Adhesion
Cell Count
Cell culture
Cell Division
Cells, Cultured
comparison studies
Dentistry
Erbium
Fibroblasts - pathology
Gingiva - pathology
Humans
Laser Therapy
lasers/therapeutic use
Microscopy, Electron, Scanning
Middle Aged
Periodontal Diseases - radiotherapy
periodontal diseases/therapy
Root Planing - instrumentation
Statistics, Nonparametric
Subgingival Curettage - instrumentation
Time Factors
Tooth Root - radiation effects
Yttrium
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Summary:Background: The application of Er:YAG laser irradiation, approved in 1997 to be used on dental hard tissues, has been investigated for periodontal therapy. The aim of this study was to analyze the biocompatibility of root surfaces treated by Er:YAG laser. Methods: Adhesion and growth of cultured human gingival fibroblasts on root surfaces treated by either irradiation with Er:YAG laser or curet were compared. Thirty single‐rooted teeth extracted because of periodontal disease were used. Calculus deposits on all experimental surfaces were removed, and the teeth were divided into three groups according to the applied treatment: group A, root planing with Gracey curet no. 3/4; group B, two irradiations with laser (60 mJ/pulse, 10 Hz; 10″ each with 10‐second interval, 3 J/cm2); group C, two irradiations with laser (100 mJ/pulse, 10 Hz; 10″ each with 10‐second interval, 5 J/cm2). Fragments (5 mm × 6 mm) were obtained from the experimental surfaces. Then, 1 × 103 cells were seeded on the top of each fragment. One, 2, and 3 days after seeding the specimens were prepared for scanning electron microscopy analysis, and the cells on the electronmicrographs were counted. The data obtained in triplicate were statistically compared by the Kruskall‐Wallis test complemented by the Dunn test (P ≤0.05). Results: Human gingival fibroblasts adhered to and grew on all treated surfaces. Group B presented a significantly higher cell count than did the other two groups at days 1 and 2. Three days after seeding the cultured fibroblasts of groups A and B reached total confluence. The cell count of group B was significantly higher than that of group C. Conclusion: The surfaces treated with 60 mJ/pulse Er:YAG laser irradiation promoted faster adhesion and growth than surfaces treated with either root planing or 100 mJ/pulse Er:YAG laser irradiation. J Periodontol 2003;74:1368‐1375.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0022-3492
1943-3670
DOI:10.1902/jop.2003.74.9.1368