SAGE transcript profiling of the juvenile cambial region of Eucalyptus grandis

SAGE transcript profiling of the juvenile cambial region of Eucalyptus grandis

Despite the importance of Eucalyptus spp. in the pulp and paper industry, functional genomic approaches have only recently been applied to understand wood formation in this genus. We attempted to establish a global view of gene expression in the juvenile cambial region of Eucalyptus grandis Hill ex...

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Bibliographic Details
Published in:Tree physiology Vol. 28; no. 6; pp. 905 - 919
Main Authors: Carvalho, M.C. da C.G. de, Caldas, D.G.G, Carneiro, R.T, Moon, D.H, Salvatierra, G.R, Franceschini, L.M, Andrade, A. de, Celedon, P.A.F, Oda, S, Labate, C.A
Format: Journal Article
Language:English
Published: Canada 01-06-2008
Subjects:
Acclimatization
bioinformatics
cambium
cell walls
cellulose
Cloning, Molecular
DNA fingerprinting
DNA Primers
Enzymes - genetics
Eucalyptus
Eucalyptus - genetics
Eucalyptus - growth & development
Eucalyptus grandis
gene expression
Gene Expression Profiling
Gene Library
genes
Genes, Plant
genomic libraries
Glycolysis
juvenile wood
lignin
nucleotide-sugars interconvertions
Plant Proteins - genetics
quantitative analysis
quantitative genetics
Reverse Transcriptase Polymerase Chain Reaction
sequence analysis
Transcription, Genetic
transcriptome
tree growth
trees
wood
wood formation
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Summary:Despite the importance of Eucalyptus spp. in the pulp and paper industry, functional genomic approaches have only recently been applied to understand wood formation in this genus. We attempted to establish a global view of gene expression in the juvenile cambial region of Eucalyptus grandis Hill ex Maiden. The expression profile was obtained from serial analysis of gene expression (SAGE) library data produced from 3- and 6-year-old trees. Fourteen-base expressed sequence tags (ESTs) were searched against public Eucalyptus ESTs and annotated with GenBank. Altogether 43,304 tags were generated producing 3066 unigenes with three or more copies each, 445 with a putative identity, 215 with unknown function and 2406 without an EST match. The expression profile of the juvenile cambial region revealed the presence of highly frequent transcripts related to general metabolism and energy metabolism, cellular processes, transport, structural components and information pathways. We made a quantitative analysis of a large number of genes involved in the biosynthesis of cellulose, pectin, hemicellulose and lignin. Our findings provide insight into the expression of functionally related genes involved in juvenile wood formation in young fast-growing E. grandis trees.
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content type line 23
ISSN:0829-318X
1758-4469
DOI:10.1093/treephys/28.6.905