Sample preparation and determination of gabapentin in venous and capillary blood using liquid chromatography–tandem mass spectrometry

Sample preparation and determination of gabapentin in venous and capillary blood using liquid chromatography–tandem mass spectrometry

An analytical method for the determination of gabapentin in serum obtained from venous blood samples has been developed using high-performance liquid chromatography (HPLC)–tandem mass spectrometry. In addition, a comparative study between capillary plasma samples and venous serum samples was carried...

Full description

Saved in:
Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis Vol. 34; no. 2; pp. 415 - 423
Main Authors: Carlsson, K.C., Reubsaet, J.L.E.
Format: Journal Article
Language:English
Published: Amsterdam Elsevier B.V 04-02-2004
Elsevier Science
Subjects:
Acetates - blood
Acetates - chemistry
Amines
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Capillaries - metabolism
Capillary blood samples
Chromatography, High Pressure Liquid - methods
Clinical drug monitoring
Cyclohexanecarboxylic Acids
Fundamental and applied biological sciences. Psychology
Gabapentin
gamma-Aminobutyric Acid
General pharmacology
Humans
LC–MS/MS
Mass Spectrometry - methods
Medical sciences
Pharmacology. Drug treatments
Pilot Projects
Veins - metabolism
Capillary blood samples
Clinical drug monitoring
Gabapentin
LC–MS/MS
Drug
LC-MS/MS
Mass spectrometry MS/MS
Anticonvulsant
Blood capillary
Liquid chromatography
Circulatory system
Quantitative analysis
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An analytical method for the determination of gabapentin in serum obtained from venous blood samples has been developed using high-performance liquid chromatography (HPLC)–tandem mass spectrometry. In addition, a comparative study between capillary plasma samples and venous serum samples was carried out. This demonstrates the potential for the use of the described analytical system using very small amounts of blood. As internal standard ( S)-(+)-α-amino-cyclohexane-propionic acid hydrate was used. Gabapentin and the internal standard are structural isomers, but have different m/ z values for the fragments after collision induced dissolution. Gabapentin has 172→154 and 172→136 transitions and amino-cyclohexane–propionic acid hydrate has a 172→126 transition which can be detected in tandem MS. Analysis of gabapentin was carried out on a C8 HPLC column using an isocratic mobile phase consisting of ammonium acetate (pH 3.0; 5 mM)–methanol (96:4, v/v). The analytical method was validated for venous serum samples. Limit of detection was 1.6 ng/ml and lower limit of quantification was 7.5 ng/ml. R.S.D. values and bias values were within the range of acceptance for all concentration levels. The method developed for venous serum samples is being used in a gabapentin monitoring study using population pharmacokinetic modeling.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(03)00572-7