Search Results - "Carlquist, Magnus"
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Engineering Saccharomyces cerevisiae for production of the capsaicinoid nonivamide
Published in Microbial cell factories (28-05-2022)“…Capsaicinoids are produced by plants in the Capsicum genus and are the main reason for the pungency of chili pepper fruits. They are strong agonists of TRPV1…”
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Flow cytometric analysis reveals culture condition dependent variations in phenotypic heterogeneity of Limosilactobacillus reuteri
Published in Scientific reports (07-12-2021)“…Optimisation of cultivation conditions in the industrial production of probiotics is crucial to reach a high-quality product with retained probiotic…”
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Recombinant yeast for production of the pain receptor modulator nonivamide from vanillin
Published in Frontiers in chemical engineering (12-01-2023)“…We report on the development of a method based on recombinant yeast Saccharomyces cerevisiae to produce nonivamide, a capsaicinoid and potent agonist of the…”
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Muconic Acid Production Using Engineered Pseudomonas putida KT2440 and a Guaiacol-Rich Fraction Derived from Kraft Lignin
Published in ACS sustainable chemistry & engineering (21-06-2021)“…Industrial lignin such as kraft lignin is an abundant feedstock for renewable chemicals and materials. In this study, a process was developed for…”
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Bioprospecting Microbial Diversity for Lignin Valorization: Dry and Wet Screening Methods
Published in Frontiers in microbiology (09-06-2020)“…Lignin is an abundant cell wall component, and it has been used mainly for generating steam and electricity. Nevertheless, lignin valorization, i.e. the…”
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Evaluation of heterologous expression in Pichia pastoris of Pine Weevil TRPA1 by GFP and flow cytometry
Published in Microbial cell factories (12-04-2024)“…The wasabi receptor, also known as the Transient Receptor Potential Ankyrin 1 (TRPA1) ion channel, is a potential target for development of repellents for…”
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Metabolic engineering of Pseudomonas putida for production of vanillylamine from lignin‐derived substrates
Published in Microbial biotechnology (01-11-2021)“…Summary Whole‐cell bioconversion of technical lignins using Pseudomonas putida strains overexpressing amine transaminases (ATAs) has the potential to become an…”
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Non-inhibitory levels of oxygen during cultivation increase freeze-drying stress tolerance in Limosilactobacillus reuteri DSM 17938
Published in Frontiers in microbiology (2023)“…The physiological effects of oxygen on DSM 17938 during cultivation and the ensuing properties of the freeze-dried probiotic product was investigated. On-line…”
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Assessment of fluorescent protein candidates for multi-color flow cytometry analysis of Saccharomyces cerevisiae
Published in Biotechnology reports (Amsterdam, Netherlands) (01-06-2022)“…•In vivo fluorescence of candidate fluorescent proteins was assessed in Saccharomyces cerevisiae.•eEGFP, CyOFP1opt and mBeRFPopt were found to be suitable for…”
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Biocatalytic potential of vanillin aminotransferase from Capsicum chinense
Published in BMC biotechnology (09-04-2014)“…The conversion of vanillin to vanillylamine is a key step in the biosynthetic route towards capsaicinoids in pungent cultivars of Capsicum sp. The reaction has…”
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Assessment of the TRX2p-yEGFP Biosensor to Monitor the Redox Response of an Industrial Xylose-Fermenting Saccharomyces cerevisiae Strain during Propagation and Fermentation
Published in Journal of fungi (Basel) (30-05-2023)“…The commercial production of bioethanol from lignocellulosic biomass such as wheat straw requires utilizing a microorganism that can withstand all the…”
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Optimization of a hybrid bacterial/Arabidopsis thaliana fatty acid synthase system II in Saccharomyces cerevisiae
Published in Metabolic engineering communications (01-12-2023)“…Fatty acids are produced by eukaryotes like baker's yeast Saccharomyces cerevisiae mainly using a large multifunctional type I fatty acid synthase (FASI) where…”
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Evaluation of Pyrophosphate-Driven Proton Pumps in Saccharomyces cerevisiae under Stress Conditions
Published in Microorganisms (Basel) (20-03-2024)“…In , pH homeostasis is reliant on ATP due to the use of proton-translocating ATPase (H -ATPase) which constitutes a major drain within cellular ATP supply…”
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Physiological heterogeneities in microbial populations and implications for physical stress tolerance
Published in Microbial cell factories (16-07-2012)“…Traditionally average values of the whole population are considered when analysing microbial cell cultivations. However, a typical microbial population in a…”
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Quantitative Flow Cytometry to Understand Population Heterogeneity in Response to Changes in Substrate Availability in Escherichia coli and Saccharomyces cerevisiae Chemostats
Published in Frontiers in bioengineering and biotechnology (2019)“…Microbial cells in bioprocesses are usually described with averaged parameters. But in fact, single cells within populations vary greatly in characteristics…”
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NADH-dependent biosensor in Saccharomyces cerevisiae: principle and validation at the single cell level
Published in AMB Express (30-10-2014)“…A reporter system was constructed to measure perturbations in the NADH/NAD + co-factor balance in yeast, by using the green fluorescent protein gene under the…”
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Exploring the potential of the glycerol-3-phosphate dehydrogenase 2 (GPD2) promoter for recombinant gene expression in Saccharomyces cerevisiae
Published in Biotechnology reports (Amsterdam, Netherlands) (01-09-2015)“…A control point for keeping redox homeostasis in Saccharomyces cerevisiae during fermentative growth is the dynamic regulation of transcription for the…”
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Increased lignocellulosic inhibitor tolerance of Saccharomyces cerevisiae cell populations in early stationary phase
Published in Biotechnology for biofuels (04-05-2017)“…Production of second-generation bioethanol and other bulk chemicals by yeast fermentation requires cells that tolerate inhibitory lignocellulosic compounds at…”
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Expression of the Bacterial Enzyme IdeS Using a GFP Fusion in the Yeast Saccharomyces cerevisiae
Published in Methods in molecular biology (Clifton, N.J.) (2023)“…Bacterial proteases are important enzymes used in several technical applications where controlled cleavage of proteins is needed. They are challenging enzymes…”
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Boronic Acid Modified Polymer Nanoparticles for Enhanced Bacterial Deactivation
Published in Chembiochem : a European journal of chemical biology (13-12-2019)“…A new method has been developed to enhance the antibacterial efficiency of traditional antibiotics. Chloramphenicol‐imprinted polymer particles were decorated…”
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