A novel weight-drop closed head focal traumatic brain injury: A candidate to translational studies?

Traumatic brain injury (TBI) is a neurotrauma with a complex pathophysiology caused by an external mechanical force. This global public health problem is a leading cause of death and disability in young adults. In this scenario, many models were developed to try to simulate human TBI. The weight dro...

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Bibliographic Details
Published in:	MethodsX Vol. 13; p. 102806
Main Authors:	Lirio, Pedro Henrique Cassaro, Gonçalves, Jessica Vaz, Filho, Walter Nunes Pontara, Amancio, Thamiris Alves, Carlini, Juliana Tancredo, Dalpiaz, Polyana Lima Meireles, Sartório, Carmem Luíza, Rodrigues, Livia Carla de Melo, Areas, Fernando Zanela da Silva
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-12-2024
Subjects:	Projectile Rats Traumatic brain injury Vital parameters Weight-drop model Weight-drop closed-head traumatic brain injury Rats Traumatic brain injury Vital parameters Weight-drop model Projectile
Online Access:	Get full text
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Summary:	Traumatic brain injury (TBI) is a neurotrauma with a complex pathophysiology caused by an external mechanical force. This global public health problem is a leading cause of death and disability in young adults. In this scenario, many models were developed to try to simulate human TBI. The weight drop model allows the investigation of the pathophysiological cascades of TBI without surgical interference. In this protocol, a new closed-head weight-drop rat model consisting of a 48.5g weight projectile that free falls from 1.10m high onto the skull of the animals was built. We classify the present TBI model performed as moderately severe due to its mortality rate. Animals from TBI and Control (Sham) groups underwent weight for 7 days and temperature assessments within 1 hour after TBI and for 7 days. Results demonstrated that the TBI group showed less body weight gain in the days after the injury. Temperature oscillations within the first-hour post-injury and on the 3rd day after injury were observed. As the results of this study demonstrated similarity to human TBI vital parameters, this new adaptation of the Weight-drop model injury can be a suitable candidate for translational studies.•We developed a novel closed head focal traumatic brain injury using a projectile.•This TBI model does not require surgical intervention.•The validation of this method demonstrates that the vital parameters of the injured rats exhibit similarities with those of TBI patients. [Display omitted]
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2215-0161 2215-0161
DOI:	10.1016/j.mex.2024.102806