Preconditioning with mono and polyunsaturated fatty acids and low-intensity electrical stimulation. Effects on skin repair in rats

To evaluate the effects of preconditioning with oils mixes containing ω3/ω6/ω9 associated with micro-currents on skin repair in rats. One-hundred and eight Wistar rats randomized into G-1, G-2 and G-3 groups were treated with saline (0.9%), mix 1 (corn+soybean oils) and mix 2 (olive+canola+flaxseed...

Full description

Saved in:

Bibliographic Details
Published in:	Acta cirurgica brasileira Vol. 30; no. 2; pp. 107 - 114
Main Authors:	Cardoso, Maria dos Prazeres Carneiro, Albuquerque, Andréa de Oliveira, Girão, Virginia Claudia Carneiro, Pompeu, Margarida Maria de Lima, Silva, Cícero Igor Simões Moura, Azevedo, Orleâncio Gomes Ritardo de, Guimarães, Sergio Botelho, Vasconcelos, Paulo Roberto Leitão de
Format:	Journal Article
Language:	English
Published:	Brazil Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 01-02-2015
Subjects:	Animals Electric Stimulation Therapy Electric Stimulation Therapy - methods Fatty Acids, Monounsaturated - therapeutic use Fatty Acids, Unsaturated - therapeutic use Fibrosis - pathology Immunohistochemistry Male Oils Random Allocation Rats Rats, Wistar Reproducibility of Results Skin Skin - blood supply Skin - drug effects Skin - pathology Time Factors Treatment Outcome Wound Healing Wound Healing - drug effects
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	To evaluate the effects of preconditioning with oils mixes containing ω3/ω6/ω9 associated with micro-currents on skin repair in rats. One-hundred and eight Wistar rats randomized into G-1, G-2 and G-3 groups were treated with saline (0.9%), mix 1 (corn+soybean oils) and mix 2 (olive+canola+flaxseed oils), respectively, in a single dose (0.01ml/g) by gavage. Next, each group was subdivided into sham and stimulated subgroups. Pulsed-wave microcurrents (0.5 µA, 0.5 Hz) were applied to stimulated subgroups for 20 min. One hour later anesthetized rats were subjected to surgery. A dorsal incision (6 cm long) was carried out and closed with interrupted nylon sutures. Samples (1 cm2) were harvested from the mid-portion of the incision on the 7, 14, 21 post-operative (P.O.) days. Variables were analyzed using Mann-Whitney/Dunn tests Significance level was set to 5 % (p<0.05). Micro-currents promoted increase of exudate and reduction of epithelialization on day 7 in G1 rats. Mixes 1/2 reduced vascularization on 7/14th days P.O. Both 1/2 mixes reduced fibrosis on day 14. Preconditioning with mix 1 led to increased expression of NF-kB on the 7th day. Preconditioning with microcurrents has pro-inflammatory effects while oil mixes 1 and 2 decrease fibrosis and vascularization in the proliferative phase of cicatrization.
ISSN:	0102-8650 1678-2674 0102-8650
DOI:	10.1590/S0102-86502015002000004