Associations of FASN gene polymorphisms with economical traits in Nellore cattle (Bos primigenius indicus)

The aim of this study was to identify molecular markers to be applied to marker-assisted selection. Three SNPs of the FASN gene were studied. PCR–RFLP was used for genotyping. The SNPs g.17924A>G, g.17860C>T and g.15603A>G all in the FASN gene were genotyped using the enzymes MscI , DdeI an...

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Bibliographic Details
Published in:	Molecular biology reports Vol. 39; no. 12; pp. 10097 - 10104
Main Authors:	de Souza, Fabio Ricardo Pablos, Chiquitelli, Milena Gandin, da Fonseca, Larissa Fernanda Simielli, Cardoso, Diércles Franscisco, da Silva Fonseca, Patrícia Dias, de Camargo, Gregório Miguel Ferreira, Gil, Fernanda Maria Monsalves, Boligon, Arione Augusti, Tonhati, Humberto, Mercadante, Maria Eugênia Zerlotti, de Albuquerque, Lucia Galvão
Format:	Journal Article
Language:	English
Published:	Dordrecht Springer Netherlands 01-12-2012 Springer Nature B.V
Subjects:	Adiposity - genetics Animal Anatomy Animal Biochemistry Animals Biomedical and Life Sciences Biometry Body Weight - genetics Bos primigenius Breeding - economics Cattle Cattle - anatomy & histology Cattle - genetics Fatty Acid Synthases - genetics Female Gene Frequency Genetic Association Studies Genetic Markers Genotype Genotype & phenotype Histology Life Sciences Male Molecular biology Morphology Polymorphism Polymorphism, Single Nucleotide Selective breeding Sequence Analysis, DNA Beef cattle Molecular markers Marker assisted selection Carcass traits Growth traits
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Summary:	The aim of this study was to identify molecular markers to be applied to marker-assisted selection. Three SNPs of the FASN gene were studied. PCR–RFLP was used for genotyping. The SNPs g.17924A>G, g.17860C>T and g.15603A>G all in the FASN gene were genotyped using the enzymes MscI , DdeI and Hae III , respectively. The animals were raised in extensive systems and belong to three lines selected for growth as part of the Selection Program of Zebu and Caracu Breeds, São Paulo, Brazil. Allele and genotype frequencies were compared between selection lines using the Genepop 3.4. Associations between polymorphisms and the traits studied were evaluated using the PROC MIXED procedure of the SAS/STAT 9.1.3. The G and C alleles were the most frequent alleles of the g.15603A>G and g.17860C>T loci, respectively. The g.17924A>G locus showed no polymorphism in the population studied. Allele and genotype frequencies differed significantly between the NeT line and the NeC and NeS lines. The g.15603A>G polymorphism tended to exert an additive effect on rump fat thickness and male yearling height. For g.17860C>T, an additive effect on male yearling height was observed. Genotype combination analysis revealed a significant effect on loin eye area. Although this study provided evidence of an association between the FASN gene and some traits, more detailed analyses are needed to obtain more efficient molecular markers.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0301-4851 1573-4978
DOI:	10.1007/s11033-012-1883-6