Acetate cellulose fibrous scaffold is suitable for cultivated fat production

Fat is an essential component of meat which contributes to its sensory characteristics. Therefore, producing cultivated fat is essential to replicate the texture, flavor, and juiciness of conventional meat. One of the challenges in obtaining cultivated fat is that once adipocytes reach differentiati...

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Published in:	Current research in food science Vol. 9; p. 100903
Main Authors:	Moreira, Amanda Maria Siqueira, Nogueira, Júlia Meireles, Carceroni, Jade, Guadalupe, Jorge Luís, dos Santos, Ana Elisa Antunes, Fagundes, Ana Maria Alvarenga, Copola, Aline Gonçalves Lio, Silva, Gerluza Aparecida Borges, da Silva, Aline Bruna, Santos, João Paulo Ferreira, Albergaria, Juliano Douglas Silva, Oliveira Andrade, Luciana de, Jorge, Erika Cristina
Format:	Journal Article
Language:	English
Published:	Elsevier B.V 2024 Elsevier
Subjects:	Adipocytes Adipogenic differentiation Cellulose acetate Cultivated fat Electrospinning Scaffold Adipogenic differentiation Cultivated fat Electrospinning Adipocytes Scaffold Cellulose acetate
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Summary:	Fat is an essential component of meat which contributes to its sensory characteristics. Therefore, producing cultivated fat is essential to replicate the texture, flavor, and juiciness of conventional meat. One of the challenges in obtaining cultivated fat is that once adipocytes reach differentiation in culture, they tend to float. In this study, we tested whether immortalized pre-adipocytes could be viable, grow, and differentiate when cultivated onto a fibrous scaffold produced by the electrospun of cellulose acetate. Our results demonstrated that the cells attach, proliferate, colonize, and differentiate into mature adipocytes in the three-dimensional fibrous structure during the culture period. Moreover, when layers of the scaffold containing differentiated cells were stacked, it acquired a characteristic similar to conventional animal fat. Therefore, this research suggests that fibrous scaffolds produced using cellulose acetate are a promising substrate for producing cultivated fat. [Display omitted] •CAN scaffolds allow cultivated fat production for food applications.•3T3-L1 pre-adipocytes remain viable and can proliferate onto the CAN scaffold.•CAN scaffolds allow 3T3-L1 pre-adipocytes fully differentiation and support long term culture.•Mature adipocytes do not float when culitvated onto the CAN.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2665-9271 2665-9271
DOI:	10.1016/j.crfs.2024.100903